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Mutant Lines
11-1918-1

11-1918-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

3 prime edge [+] show sequence

Chromosome: 3; Position: 16817680 [Map]; Strand: w
- AT3G45780.1 - [+] show detail - phototropin 1
  Distance: 0; Region: intron1 (5UTR)
- AT3G45780.2 - [+] show detail - phototropin 1
  Distance: -763; Region: promoter

TATATGAATG TATATGCATG CAGGTAACAT TATTAAATAT GGTTCAAAGA 
GTTATGGTTT ATGGGGCCAT AGATGAATGA AGATATGAAC TGGAAAATAC 
GCCAATTTTG AGAGTGACAA TTCCAAATGA AGCNGGGGGT AAAATTGAGA 
AAACGACACA AAGGTGTAAA GTTTCAATGC TTTTTTGAGT TTTTAGTACC 
ACACAACACA TGCATCTTCT AAGGGTGGTT TAGTCAAAAA TGGACAGTTG 
TTCTACTACA AAAACAGTAG CTGAACCGAT TCTGTTCGAT CGATCACAGA 
TTTCAACTAA AAATCTTTGT CTTTTTTTCA TGTTCTTGTC ATTTTATCAA 
TTTCCACATA AATGAACTTT GTAAAATAGT TTATCACTCT ATTTAAATGA 
GAACAAATAA AAAGAGTAAA AAATTTCATG GTAAATAGGA AAATCTATTT 
TTATTTAAAA AAATTGAAAT ACATATCTTT ATGAATTATG ATTGGAGAAA 
TTTTAAATCG AATT

AT3G45780.1

Model type: Protein coding
Short Description: phototropin 1
Curator Summary: Blue-light photoreceptor. Contains a light activated serine-threonine kinase domain and LOV1 and LOV2 repeats. Mutants are defective in blue-light response. Mediates blue light-induced growth enhancements. PHOT1 and PHOT2 mediate blue light-dependent activation of the plasma membrane H+-ATPase in guard cell protoplasts. PHOT1 undergoes blue-light-dependent autophosphorylation. At least eight phosphorylation sites have been identified in PHOT1. Phosphorylation of serine851 in the activation loop of PHOT1 appears to be required for stomatal opening, chloroplast accumulation, leaf flattening, and phototropism, and phosphorylation of serine849 may also contribute to the regulation of these responses. Phosphorylation-dependent binding of 14-3-3 proteins to the Hinge1 region of PHOT1 appears to require serine350 and serine376.
Computational Description: phototropin 1 (PHOT1); FUNCTIONS IN: protein binding, protein serine/threonine kinase activity, FMN binding, kinase activity, blue light photoreceptor activity; INVOLVED IN: in 8 processes; LOCATED IN: internal side of plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: PAC motif (InterPro:IPR001610), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), PAS fold (InterPro:IPR013767), PAS (InterPro:IPR000014), PAS-associated, C-terminal (InterPro:IPR000700), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719); BEST Arabidopsis thaliana protein match is: phototropin 2 (TAIR:AT5G58140.1); Has 112705 Blast hits to 107868 proteins in 3419 species: Archae - 296; Bacteria - 19357; Metazoa - 40871; Fungi - 12339; Plants - 20501; Viruses - 442; Other Eukaryotes - 18899 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G45780.2

Model type: Protein coding
Short Description: phototropin 1
Curator Summary: Blue-light photoreceptor. Contains a light activated serine-threonine kinase domain and LOV1 and LOV2 repeats. Mutants are defective in blue-light response. Mediates blue light-induced growth enhancements. PHOT1 and PHOT2 mediate blue light-dependent activation of the plasma membrane H+-ATPase in guard cell protoplasts. PHOT1 undergoes blue-light-dependent autophosphorylation. At least eight phosphorylation sites have been identified in PHOT1. Phosphorylation of serine851 in the activation loop of PHOT1 appears to be required for stomatal opening, chloroplast accumulation, leaf flattening, and phototropism, and phosphorylation of serine849 may also contribute to the regulation of these responses. Phosphorylation-dependent binding of 14-3-3 proteins to the Hinge1 region of PHOT1 appears to require serine350 and serine376.
Computational Description: phototropin 1 (PHOT1); FUNCTIONS IN: protein binding, protein serine/threonine kinase activity, FMN binding, kinase activity, blue light photoreceptor activity; INVOLVED IN: in 8 processes; LOCATED IN: internal side of plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: PAC motif (InterPro:IPR001610), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), PAS fold (InterPro:IPR013767), PAS (InterPro:IPR000014), PAS-associated, C-terminal (InterPro:IPR000700), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719); BEST Arabidopsis thaliana protein match is: phototropin 2 (TAIR:AT5G58140.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR