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12-5195-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24200947 [Map]; Strand: c
      • AT5G60100.2 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.3 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.1 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
    Flanking Genome Sequence: 12-5195-1 - 5-prime edge
    TCTGTAGAGT TTATAAGAAA TTTGAGAAAG TAGGAGATCT TTACAATCAT 
    CTAATGAATA AAAGAAACTG TGTTCAAAGT TTGTTAACTA CTTACCAAGT
    ACTGTGATAA TTTTCCATTG GAACATTGAT AGCTTTTTGA TTTTAGAAAC
    AACAAGTTTC AGATTGTTTT CTAAAGCTGA GTGTGTGCTA CTATTGAAAT
    TTGATTTGGC TTGTTTAGTT TTTCTTTGGA CAAGTTTGAT GATTTATCCA
    AATTCAGTTT TTTATTTTTT GGTTCTGGTG TGAGACTGTG ATCTTCTTAC
    GGAATGTTGC TTTTTTTTTN GTTNCNCGTT CCCTTNTNGG GCTGAGGTTT
    NGGNGAAGGG ATTAAAATAA GTTGGGGGAA ANAAAAAATG TNCAAGNGTT
    ATGNCAGNGT AAAGGGGAAN TTTNNGCAAA NTCNTACCNG TTNCTNNAGG
    CTNCTATCTN TCCTANCTGG TCCATGAAGG CNGGNCCTNN ACCCTTTCCA
    NNNAAAATAT TATGNTTCCT AAAAANGNTG NTGTTAAAAA ACNTCGAAAN
    CGTGGNTGNT GANNNTNGGA NAACCGAGNG GGCTATNGTT CNNGCNCNTN
    NCTGANNAAC ATGGANTTCN CGANTNGGNA NNATNCNNNT NAAAAACNAC
    CGANCAGTGN NNGANATANN NANNNCNCAN CGNGNNANCN GGGAGCNNCC
    TGGTTTGGNA ACNTNGNAGA GNGTTTNNCC CGNTNATANA N
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24201063 [Map]; Strand: c
      • AT5G60100.2 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.3 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.1 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: -891; Region: promoter
    Flanking Genome Sequence: 12-5195-1 - 3-prime edge
    TCTACAGAAC CCAGATACAA AATCAAACTC TAAGGCTCTA AGCTATAATA 
    CTTAATGAAA TAAGCACAAA ATTCAAACAG CAGCAGATTG TAAAAGCGAC
    AAGACAAACA AAAGCAAACC TTTTTCACAT AATCCATTGA TGAACACCAC
    CACCACCAGA ATCTCTCACC ATATCTGACT TGAAAAACCA AATTACAACC
    AAAAAAGAAC AGATTTTTGC TTAATCTGAT TTGCTTAGCA TTTAATCACC
    TAATCATTCA AAAATGTTTT CATTCGTATC TATTTTTCTT TTACCAACAA
    CTTTACAGAA CCAAATTGCT ACTAAAATAG AATAAAAAGA AAGAAAATTT
    AAAAAACTTG ATAGAGAATC AGAGGAAGAG AAGATTGGGA TTTTTTGGGA
    GGTGGTAGAA CAAAAAGGTC AGAGAAGATA TTATTTTGGA GTTTGATGAC
    GTGGCAGAAC TAACCTACGT GTCAAGTAGG ACCAGATAAA TTTACGCATG
    CTGAGATTAG TGACATGGAC AAAATCAAAA GCT

AT5G60100.1

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60100.2

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1).
Link
InterPro Scan - TAIR

AT5G60100.3

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1).
Link
InterPro Scan - TAIR

AT5G60110.1

Model type
Protein coding
Short Description
pumilio 18
Curator Summary
Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts.
Computational Description
pumilio 18 (PUM18); FUNCTIONS IN: RNA binding, binding; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Pumilio RNA-binding repeat (InterPro:IPR001313), Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: pumilio 19 (TAIR:AT5G60180.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR