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13-0643-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3579466 [Map]; Strand: c
      • AT1G10750.1 - [+] show detail - Protein of Unknown Function (DUF239)
        Distance: -992; Region: promoter
    Flanking Genome Sequence: 13-0643-1 - 5-prime edge
    GCTGACAGAA GGAACATATA GGGAAGAGAG TAAAGAGAAA AAGCGAACTT 
    CAAGAATCAA CAATAAGAAA AAGTAAATTA TTTGCAAATT CCTCTTTTTG
    TCTCTTAAAA GATACAAAAA ATGTTTTTTT TTTTAANCCC CCAGAAANNG
    CNACCTACCC CAANATAAAN CANGAANAAA AACCCCANAN TTTTGCCNGN
    CTTTNTCNTA AAAAACNTAN CNCCCCCAAA ACTTTCCTTT NTTTTNGGCT
    NTNTCCNTTT TTTCAANCAA NCNTANTNTN TTTCAGGCTT TGTTTTGCTA
    NTNGGNAACN TTTCCCCNCC CCCNNNTTTT TTTTTTTTTT TTNCCCNCCT
    NACCTTNTTT TANTTGGATT CCCCTTTNAN CCCCNNNNGG NANNNAATTT
    TNGCNTTTTT TTTTTTNCTN NNAAAAAAAA NNTTNNNNTT TCCNAAAAAN
    CCNNAANCCC CCGGGGCCAA NNAAAGGGTT NNGGGCNNNN NTTNGGGNNG
    GGNNAANNNN CCCGGGNCGG NNAANNCCCN TGCCCNTTTT
  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3579582 [Map]; Strand: c
      • AT1G10750.1 - [+] show detail - Protein of Unknown Function (DUF239)
        Distance: -1108; Region: intergenic
      • AT1G10760.1 - [+] show detail - Pyruvate phosphate dikinase, PEP/pyruvate binding domain
        Distance: 1452; Region: intergenic
    Flanking Genome Sequence: 13-0643-1 - 3-prime edge
    CTGTCAGCTA CTCTCTCTCT TTTTTCTTTT TCTTTTACAA CATTTTTTCG 
    AGAGCATTAT ATCTTCATAA GATTTTAACT TCTACTTGAA AAATATACAT
    ATAAATGAAC AACTACGTGA AGAGTATAAC TGTGGTAGTG GAAGAGTATA
    ACAAAGTTTA GTACTAATTA ATTGTAAATA ATAGATTAGA TCATTTTAAT
    TTACTAAGTA GTTTGTGTTA GGGACATGGG AGTGTTGGCA CGCGAGGCTG
    AGTATTAGCA ATGCATGCTC GAGTGCTTAT GTTGGGGACC CATCCATGCA
    TGCCATCCTT AATTTATTCC CACCAATTAT CATTACCTAT CCCTTGATTA
    TGCATGGTAT ATTTTTCAAT ATTTAAAACC TATACAGGAT AAGAACAGAA
    ATAATGTAAT TTGAATACGT ACAGAGTTAT ATGCTAGTAT TTACAAATGG
    TATTGGTATG TATATTGTTT TCTGCTTCAT AGTTACAACC ATTTGGACAT
    GGGCATGTTA AAAATTTAAT ATGTGGCAAA CATAAATTTG ATACTTGTCG
    TCATGGCTTG TAAATAATAT GCAATGTAAA CCTTCAAAAA TTGAGATTCA
    GCATCTATAA TATATGTTGC ATAACTTGNG AGGTTCAAGA TCCTAATCAT
    ATTATAAAAA AATCCATATT CTTCCTTCCA CATTTCCACC ATTAGTGGAT
    TCTTAAGAGA GAANAAAAAA TTAAACACCC TTAACTTGTT AGTTAAGCAT
    TGNNCNCCAA ANTCGAT

AT1G10750.1

Model type
Protein coding
Short Description
Protein of Unknown Function (DUF239)
Computational Description
Protein of Unknown Function (DUF239); INVOLVED IN: N-terminal protein myristoylation; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF239, plant (InterPro:IPR004314); BEST Arabidopsis thaliana protein match is: Protein of Unknown Function (DUF239) (TAIR:AT1G23340.2); Has 789 Blast hits to 744 proteins in 31 species: Archae - 0; Bacteria - 15; Metazoa - 0; Fungi - 10; Plants - 764; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G10760.1

Model type
Protein coding
Short Description
Pyruvate phosphate dikinase, PEP/pyruvate binding domain
Curator Summary
Encodes an α-glucan, water dikinase required for starch degradation. Involved in cold-induced freezing tolerance. Mutations that eliminate the GWD protein or affect the dikinase domain of the enzyme dramatically reduce both the amount of phosphate in the amylopectin and the rate of starch degradation. Mature leaves of these mutants accumulate amounts of starch up to seven times greater than those in wild-type leaves. NMR analysis of the mutants, suggests that the gene is specifically involved in the phosphorylation of the glucosyl residues of starch at the C6 position.
Computational Description
STARCH EXCESS 1 (SEX1); FUNCTIONS IN: protein binding, alpha-glucan, water dikinase activity; INVOLVED IN: cold acclimation, response to symbiotic fungus, response to trehalose stimulus, circadian rhythm, starch catabolic process; LOCATED IN: mitochondrion, chloroplast stroma, chloroplast, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Pyruvate phosphate dikinase, PEP/pyruvate-binding (InterPro:IPR002192); BEST Arabidopsis thaliana protein match is: phosphoglucan, water dikinase (TAIR:AT4G24450.1); Has 2623 Blast hits to 2596 proteins in 1116 species: Archae - 196; Bacteria - 1989; Metazoa - 24; Fungi - 8; Plants - 221; Viruses - 0; Other Eukaryotes - 185 (source: NCBI BLink).
Link
InterPro Scan - TAIR