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13-0759-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25388805 [Map]; Strand: c
      • AT5G63380.1 - [+] show detail - AMP-dependent synthetase and ligase family protein
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 13-0759-1 - 3-prime edge
    CAACTGAGCC ATATTTTACC ATCTCTTCAG GTCCAAACGT TGAGGCAGCT 
    GGTCCCGAGC TCTCTGTCAA GCCATAGCCC TGTAAGCCAT CACCAAACCA
    GAAAGTTAAA AGTCATATAA ATGATTCAAA ATCTTGATGA AAACCAAGTA
    TCATGAAACT GGACACAGTC CTATATACAA AGTCATATTT TGACACTAAC
    TGTCTTATCA GTTATTACCT GTACAATATC TACATCTGGG AATTTCTGCT
    TAAACCTCTC TGCGATGTCT TTGCCGAGTG GAGCTCCTCC GCAGCCAAGG
    GAACGCAACG ACCGGAGATC GTACTTCTTC GTGAGCTCCG ATTTGACCAA
    CGCTACAATC AACGGAGGAG AGACTGGCAT ACCAGTAACC TTATATTTCT
    CCACCGCCTT AAACATCGCC TCGAGTTCAA ATCTCCCTAA AAGCACCAAT
    GTCTCTCCAA GCGAGATGGC TCGAATCATC ATCATGAAAC CAAACACGTG
    GAAGAGCGGA AGCGAGAACA GTCCAACGCG ATCGTAATTA ACCGGATCTT
    GGAGAGTCCG TTGGTGAGAT ACGGCGGTCG AGGCGATTAG GTTACGGTGA
    GTGAGCAAAA CGCCTTTGAC CCGCCCGGTT GTTCCANAGG AAAAGAGGAT
    AGCGGNAGGG TCCGATTNGG TTGACCTGAA CCTGAAATGG NNTAACCCGT
    GANNAATCCG ATCGATTTAA CCNG
  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25388689 [Map]; Strand: c
      • AT5G63380.1 - [+] show detail - AMP-dependent synthetase and ligase family protein
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 13-0759-1 - 5-prime edge
    CTCAGTTGGT CGTATCTCTG AGAATATGGA AGCCAAAATT GTTGATCCAT 
    CCACCGGAGA ATCCTTGCCA CCGGGAAAAA CTGGTGAACT CTGGCTCCGA
    GGACCAGTCA TCATGAAAGG TATCATAAAA CATATGCAAT AGTTGAGGAA
    CATGCAATGT GCTGAGCTGG TGTAAACTAA ATGACTTTGC ATATAAGAAT
    CTAGGACTGT CAAATTATAT CAAGACTTTG ACCATTAATG TGACTTTGCA
    TATAAGAATC TAGGACTGTG TCAAATTAAA TCGAGACTTG TGACTTTGCA
    TAGAAGAATC TAGGACTGTG TAACAAATGC AATAGTTTGA GGAACATTCA
    AGAGCTTTCA CTAAATGACT TAGCATATAA GAAGAAATCT AGGACTCAGT
    CGGATTTCAT GATCCTTGAA GTCTAATGTC TTCATCAAGA TTTTGATCAT
    TGATGTGACT ATAAACCTGT GATTAATGGG CACAGGTTAT GTGGGAAACG
    AGAAAGCGAG TGCGGAGACA GTAGACAAAG AAGGGTGGTT AAAGACTGGT
    GATCTCTGTT ATTTTGATTC GGAAGATTTT CTATATATTG TTGATCGGCT
    AAAGGAGCTA ATCAAATACA AGGCTTATCA GGTAAATTTT ACGAGTCAGA
    AAAAGAAGAT ATAGTCTTTA AAGTT

AT5G63380.1

Model type
Protein coding
Short Description
AMP-dependent synthetase and ligase family protein
Curator Summary
Encodes a peroxisomal protein involved in the activation of fatty acids through esterification with CoA. At5g63380 preferentially activates fatty acids with increased chain length (C9:0 to C8:0) and thus shares characteristics with long-chain fatty acyl-CoA synthases. Also able to catalyze the conversion of OPDA to its CoA ester and is therefore thought to be involved in the peroxisomal β-oxidation steps of jasmonic acid biosynthesis.
Computational Description
AMP-dependent synthetase and ligase family protein; CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: OPC-8:0 CoA ligase1 (TAIR:AT1G20510.1); Has 81844 Blast hits to 74712 proteins in 3773 species: Archae - 1219; Bacteria - 53606; Metazoa - 3425; Fungi - 4605; Plants - 2698; Viruses - 1; Other Eukaryotes - 16290 (source: NCBI BLink).
Link
InterPro Scan - TAIR