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13-0990-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3751185 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: intron7 (CDS)
    Flanking Genome Sequence: 13-0990-1 - 5-prime edge 
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCAGCCTTC AAACGAGCGT CAAGCCAATC TTAAACCAAG TAATTTAAAA 
    CTTACTTGTG TGGACAAGCG ATAAGATCGT TGCATTCTGT CCACGAAGAT 
    AGATCGTCGT GCCATAATCC CTAATATATG ATAACCATTA TAAAGATTCT 
    TAAAATTATA GTAAAATATG AATAAATTAG TGTCTATTAC ATTGGTGATG 
    TTCTTCTCAA GATCCTCTTG GAGAAGATCC AAGTTCTTGT CGTAGTTTTC 
    TTTTAGAGCC GTGAGAATGA TCTCTCTATC CCATACCTAC ATATTTTGTT 
    TCCTTCGTTA ATTTTTTTTT NGTTTTCANG ANNAAATTTA ANGTNGAANG 
    ANACAAAANA ANAAGCTN

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3751168 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: intron7 (CDS)
    Flanking Genome Sequence: 13-0990-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCC 
    TCTAGCTACA AATTTTGATA CTTTTTCTGG TCCAAAAATC TTACAAAGAT 
    ACTGAAAATG AAATAATAGG TTTTAAACTT TTAATTTATT TGGAGTTGGA 
    TAGGATTAAG TTTCACTAAC TTCCAATTCA AAGTC

AT1G11190.1

Model type
Protein coding
Short Description
bifunctional nuclease i
Curator Summary
Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
Computational Description
bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).
Link
InterPro Scan - TAIR