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13-3543-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4786826 [Map]; Strand: c
      • AT5G14800.1 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron4 (CDS)
      • AT5G14800.2 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: intron4 (CDS)
    Flanking Genome Sequence: 13-3543-1 - 3-prime edge 
    ATTAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCAAAGC 
    ATTTGACAGC TTCAGAGAGA AACAGAAATC ACAAACAAAA TTCAGAAATA 
    TCACAAATGC GAACACTCTG ATAAACTATA CCTGAAGCTG CCTCACCAAC 
    AGCGGCAGGT GTATTAGGCA TCACCCTTAT GAATCGATCT TGACCAGACC 
    ATTCCTAAAA ACAAAAAGGA GAGACCTTTA TGTTTTTTAA CCAAATTGAG 
    CTCTAGAAAC TCACAATGCA ACACCATGGA TAGTGACAAC TTAAATGGCA 
    AATGTATAGG AGTATAGATG ATGCAAAACC TGTAAATCAT TCAACTTGAT 
    TCCAGCTGCA ACAGAAACCA GAATCTTATT CTTTGAAAGC T

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 4786747 [Map]; Strand: c
      • AT5G14800.1 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: exon5 (CDS)
      • AT5G14800.2 - [+] show detail - pyrroline-5- carboxylate (P5C) reductase
        Distance: 0; Region: exon5 (CDS)
    Flanking Genome Sequence: 13-3543-1 - 5-prime edge 
    CATTTGCCAA TTATGTTGTG TGTATATAGT TATGAGCCTT GGCACAGGAG 
    CAACGGAAGA GGATGGAGCA ATTGTTGCTA AGTTGTTTGG CGCGGTGGGG 
    AAGATATTGA AAGCTGATGA GAAAATGTTT GATGCNTGTC ACTGGTCTCA 
    GGTACAGGAA AACACNTGTA GCTGCTGAAA TGCTTGAGTT GTTCAAGTCA 
    CTAATCAAGA AAATTTCAAA CTTTTTACAG TGGAAGTGGA CCAGCATACA 
    TATTTTTAGC AATTGAAGCT

AT5G14800.1

Model type
Protein coding
Short Description
pyrroline-5- carboxylate (P5C) reductase
Curator Summary
Delta 1-pyrroline-5-carboxylate reductase, catalyzes the final step in proline biosynthesis from glutamate and ornithine.In situ hybridization indicated that under normal growth conditions, the highest concentration of P5CR transcripts occurs in the cortical parenchyma, phloem, vascular cambium and pith parenchyma in the vicinity of the protoxylem. Single gene in Arabidopsis.
Computational Description
pyrroline-5- carboxylate (P5C) reductase (P5CR); FUNCTIONS IN: pyrroline-5-carboxylate reductase activity; INVOLVED IN: response to salt stress, response to heat, proline biosynthetic process; LOCATED IN: cell wall, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: NADP oxidoreductase, coenzyme F420-dependent (InterPro:IPR004455), NAD(P)-binding domain (InterPro:IPR016040), Delta 1-pyrroline-5-carboxylate reductase (InterPro:IPR000304); Has 7885 Blast hits to 7882 proteins in 2434 species: Archae - 115; Bacteria - 5234; Metazoa - 373; Fungi - 241; Plants - 70; Viruses - 0; Other Eukaryotes - 1852 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G14800.2

Model type
Protein coding
Short Description
pyrroline-5- carboxylate (P5C) reductase
Curator Summary
Delta 1-pyrroline-5-carboxylate reductase, catalyzes the final step in proline biosynthesis from glutamate and ornithine.In situ hybridization indicated that under normal growth conditions, the highest concentration of P5CR transcripts occurs in the cortical parenchyma, phloem, vascular cambium and pith parenchyma in the vicinity of the protoxylem. Single gene in Arabidopsis.
Computational Description
pyrroline-5- carboxylate (P5C) reductase (P5CR); CONTAINS InterPro DOMAIN/s: NADP oxidoreductase, coenzyme F420-dependent (InterPro:IPR004455), NAD(P)-binding domain (InterPro:IPR016040), Delta 1-pyrroline-5-carboxylate reductase (InterPro:IPR000304); Has 7429 Blast hits to 7426 proteins in 2378 species: Archae - 102; Bacteria - 5104; Metazoa - 368; Fungi - 229; Plants - 70; Viruses - 0; Other Eukaryotes - 1556 (source: NCBI BLink).
Link
InterPro Scan - TAIR