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13-4384-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3750479 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: intron8 (CDS)
    Flanking Genome Sequence: 13-4384-1 - 5-prime edge 
    ACTCCTCCCT GAACTATTCT CTTCATCACT ATTGGCAACC TTGTATTGAA 
    ATATTCTTCT GCAAAATTAT TTATCGGAAA TTATTAAACA AATCCTTTAG 
    CTTTCTTATA AAATTATATA TTAGACAGTC ATATATCCCA CACAAAAAAA 
    GTAGTGACTA TTAAAAATCA TTAATCCGAT TAATTAAACT TCATTTGGTC 
    GGGTTTATTT TTAAATATTT TTGTTAGCGT ACGAGAAAAA GAAGAAGCAA 
    ACAACGTACC TGATAACGTT TCACCAGACT TGACGCCTTT GTATCCCCAT 
    TTACAAGCTA ACTTTATACT CTCTGAAGCA TACCTGCGTA TACAATTAAA 
    ATTAGTTCAA TATTTTATAT AATGTTATTC GAAATTGGAT ATTTTCTCCA 
    CCCAAACAAA ACCTACTTAA GAATCTATAA TTATGATGTT AATATTTTAG 
    TTTTCAGTGG TAAACTTAAT AAGATTCTGA TCATGAATAC ACTACATATA 
    TCGAAACACA AAAGGTTCGA GTCTAATTAT GTTTCATAAG AAATCTCACA 
    TCGGAAATAA GATGTGATAT AAGGTAATAT ATATGGGTAC ATTAGGTCAA 
    CCCCACTAAT CATGGTAAAC TACTATTAAT TGACCTTTGA ATNGGAANGT 
    TAGTGAAAAC TTANTNCTAT CCANCTCCAA ATAAANTAAA NGTNNAAACC 
    CNTTTNNTNC NT

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3750410 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: exon9 (CDS)
    Flanking Genome Sequence: 13-4384-1 - 3-prime edge 
    GAAAACGGTA GAGGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCGTG 
    GGAGTTAGAC TAGCCATGAT ACTAAACCGG GTTTTTAGTG ACGATCATGC 
    TATTGCTGGT GTTGCTGCCA CTTGAACCAA ACCCGACATA CCGGGGCATC 
    AAAGCATTTG ATTAAGAGAT TATTTGATAC ATTCACAAAA TTAATTAAGG 
    CTTGATCAGA CATTCTTTTC TTTTAGTAGC TTTATCTATG TGACAGCTAA 
    TGCCTGTGGA CTGCTTTGTT TAGAAGTGTT TAGCATTAGA TCATATGCTA 
    ATTCAATGTT ATTAATTCAT CGTATGATCG TTTTAGAGAT AGTGGTTCTA 
    AGAAAATAAA CTAGATTATT TCATTCAAGT AAACCTAGTT CATCTATAAA 
    TATTTGATTC CAGTCTAAGA CAAATGAGAC CAAAAGCTAA ATATTATAGA 
    TCAAATTGAT AAAGTAAATA AGAAATCTAG CATCTAGAAA GATCACAATA 
    TATATATTTT AACAAGAGAA ACTACATAGG ATTTGTACAA AAATGAAATG 
    ACAAAATGAA ATTGAACTCA ACAAACTAGA CACTACAAGA TCACCAATCT 
    TATCTCACTC CAATAAACCA CAGAAAATTC TAAAAAGTGA ATGAAAAATA 
    TTTCTGNGGG GAAATGAGAA GATAGAANAT TAAGTTTAGG ATC

AT1G11190.1

Model type
Protein coding
Short Description
bifunctional nuclease i
Curator Summary
Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
Computational Description
bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).
Link
InterPro Scan - TAIR