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13-4521-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3752274 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 13-4521-1 - 5-prime edge 
    GTCAGGCCAC ACACACAATG CTGATAAATC TCCTTTCACG TAATCCGGTA 
    ACAGATTCTC TACTACATGT GCTGGTCCGG CTTCTAAAAG ATTCTGGTCA 
    CACCACAAAA ACACACACAT ACGAACTTTT GTTATGAATC TTATAACGGT 
    CAAATGACTC AGATCTCTAC CAAAAAAACT TGGAATTTGA TATGCCATTA 
    GACATGTTTG TATTCATGAT CATCTACATG AGATGTTAGG CATGGTAACG 
    TATAGCAATT TAAAAGTCAA TCCTAATTAA AAATTTGTAG GTATATCTCA 
    CTAATACATA TATAAGACAA GAATAACCAA AATAGTTGCT TCAAACAATA 
    GATCATTAAC TTAATTACCT GAGCAATTCT ACAAGTAAGA ATATGACCTT 
    CTTTGCTCCA GCTTCGGACA GAAGAAACCG AACACAAAAT CAGTATACCT 
    AATACAAGAA TCAACCTCGT GGATGATCTA AAAGCCGATG CCATATCTTC 
    AAAGTTTGAA ACTTATATAA TGATAAACTC TTTTGTATAA AAATGTCTTC 
    TCTTGTCTAT AACAACCTCA TCCGAATTTG AAAAAGAAAA CACACGGAGA 
    GATTATAAAT TGGAGAGACA AGAATGGGAC TTACAAGTTA GGTTAACCGG 
    AAGCACCATA TATGATGGTN GAGTTGGTGT GGCANCCAGA ANATTGNNAG 
    TCTCATANGT TC

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3752158 [Map]; Strand: w
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: 13-4521-1 - 3-prime edge 
    GGCCTGACCA GATCCGACAT TGGTACAAGT ATCGTTGGAC CAGCCATCTC 
    CATTACATCG ACACTCCCGA CCAAGCCTGC TCTTACGAAT ACTCTAGTAA 
    GTCACAACCG AGACATTTTC AGATAACCTT AATCCGTTTT CTAATTATCT 
    TGAACCGGAG TTAACCAAAA AATCAATTAC AAATACCAAA CCGGATTAAA 
    AACAGGGGAT TGTCATGATC AACATGGATT GAAGGATATG TGTGTGGATG 
    GAGCAATCCA GAATTTCACG TCTCAGCTTC AGCATTACGG TGAAGGAACA 
    TCTGATCGTA GATGTATGTC ATCATTTTCA TTTATTTCAT ATAATGATGA 
    TATCCAAAGT GTAACTGCGT ATTTTGTATT TTGATGCATA ACTTAAGTTT 
    TTAAAATTAT AATATATCCT TGTTCAATCA CATAGATAAC ATGACCGAAG 
    CCCTTTTGTT CTTGTCTCAT TTCATGGGAG ATATTCATCA GGTTTATTAC 
    TCATCATCGA TTCATTTCAC ACCTCCACAC ATATAGCTCT ATTTCCATGT 
    TAAATATTTA ATTAACATGG TTTTTTTTTT TTTTCTTTAA AANCCCGNNC 
    CGNGGGGATT CCNNGNGNNN ANGGGGGAAA CCCNTTNTTT TTNNNGGGGN 
    NAAAACCAAA NNCCNTTTTN NTTTNNGNAN CNNNNNNNCG GGGGNCNNNA 
    ANNCCGNNNN CCCNTTAN

AT1G11190.1

Model type
Protein coding
Short Description
bifunctional nuclease i
Curator Summary
Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
Computational Description
bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).
Link
InterPro Scan - TAIR