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Mutant Lines
15-4225-1

15-4225-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 1; Position: 7604302 [Map]; Strand: w
  - AT1G21650.3 - [+] show detail - Preprotein translocase SecA family protein
    Distance: -73; Region: promoter
  - AT1G21651.1 - [+] show detail - zinc ion binding
    Distance: -87; Region: promoter
Flanking Genome Sequence: 15-4225-1 - 5-prime edge
```
CCCGAGGGTA GTTCTGTATA CCTAGTGTGT ATATACATGA AAACTATTTG 
TGTTATAAGT CATCAATTTT CTAAAATGTC TCTTTAGTCT GGTGGTAAAG 
TCTTCCATAT GAGCTCATAA TTTATGGGCC GGCTCTGATT TCTGATGAAG 
TGAAGTGCAT TTGCATGGTA TATTGTCATC TTACCTCAAT GCCAATATAT 
TGCGGATC
```

3 prime edge [+] show sequence

Chromosome: 1; Position: 7604237 [Map]; Strand: w
- AT1G21650.3 - [+] show detail - Preprotein translocase SecA family protein
  Distance: -8; Region: promoter
- AT1G21651.1 - [+] show detail - zinc ion binding
  Distance: -22; Region: promoter

ATGAAAACGG TAGAGGTATT TTACCGACCG TTCCGACCGT TTTCATCCGT 
GCCCTCGGGA CTAAATGTTG TTTTAGTTAA AGTCTAAAAC GTCGTCGGAT 
TAACTCAGTT GTTCGTCGCA TCGAATCATC GGAGGTGATG GAGGAACCGG 
AGTGTCCGGT GTGTCTACAG TCTTACGACG GCGAGTCTAC TGTCCCGCGC 
GTACTCGCCT GCGGACACAC GGCGTGCGAA GAATGCTTAA CAAATCTACC 
CAAGAAGTTT CCGGACACAA TCCGATGTCC TGCTTGCACC GTTCTCGTCA 
AGTTTCCACC TCAAGGACCA TCAGCTCTCC CCAAAAACAT CGATCTTCTC 
AGATTGTTCC CTTCGATTTC GAAGCT

AT1G21650.3

Model type: Protein coding
Short Description: Preprotein translocase SecA family protein
Curator Summary: Encodes a component of the thylakoid-localized Sec system involved in the translocation of cytoplasmic proteins into plastid. Loss-of-function mutations result in arrest at the globular stage and embryo lethality.
Computational Description: Preprotein translocase SecA family protein; FUNCTIONS IN: ATPase activity, zinc ion binding, P-P-bond-hydrolysis-driven protein transmembrane transporter activity, ATP binding; INVOLVED IN: intracellular protein transport, protein targeting, protein transport by the Sec complex, protein import; LOCATED IN: internal side of plasma membrane, membrane; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), Zinc finger, RING-type (InterPro:IPR001841), SecA, preprotein translocase (InterPro:IPR022491), WD40 repeat (InterPro:IPR001680), SecA motor DEAD (InterPro:IPR014018), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), SecA Wing/Scaffold (InterPro:IPR011116), WD40-repeat-containing domain (InterPro:IPR017986), SecA preprotein, cross-linking domain (InterPro:IPR011130), SecA DEAD-like (InterPro:IPR011115), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), SecA conserved site (InterPro:IPR020937), WD40 repeat, subgroup (InterPro:IPR019781), SecA protein (InterPro:IPR000185); BEST Arabidopsis thaliana protein match is: zinc ion binding (TAIR:AT1G21651.1).
Link: InterPro Scan - TAIR

AT1G21651.1

Model type: Protein coding
Short Description: zinc ion binding
Computational Description: zinc ion binding; FUNCTIONS IN: zinc ion binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), Zinc finger, RING-type (InterPro:IPR001841), WD40 repeat (InterPro:IPR001680), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: Preprotein translocase SecA family protein (TAIR:AT1G21650.3); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR