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52-0665-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 12854653 [Map]; Strand: w
      • AT4G25000.1 - [+] show detail - alpha-amylase-like
        Distance: -808; Region: promoter
      • AT4G25010.1 - [+] show detail - Nodulin MtN3 family protein
        Distance: 0; Region: exon6 (CDS)
    Flanking Genome Sequence: 52-0665-1 - 3-prime edge 
    TCCTGACGTG AGTCATCCTA TTAAAACTCA TGGTGGTGAC TTAGAGGACC 
    AGATGGACAA GAAAATGCCA AACTAAGTCA AGATTTAGTT TTCAATTATG 
    AATTAGTGAT TGACTTTAAT GTTGAACTGT TGAGTCTGGT TTCCTAATTT 
    GTCTTTTATT TTCTCATCAT CTTCTTTCGA AATATCTCGA AATTGTACTA 
    TCCTTCAAAA ATTTATGTGC GTCGTTTAGA ATTTTGTAAA ATAGTTTTTG 
    ATGCCTCTGT TCTCAGTAAT ACTTAAAAGT TTATTAGTCC GGCTAATTAA 
    AAAGGAAATG AAAATTCAAA GTAAGTTAGA TAAACATGAT CATTCACAGG 
    TCAGATGTTT TTAAAAAAAT CATTATGGTG TACATCACAT GTAGACAATA 
    CTTCAGAATT

AT4G25000.1

Model type
Protein coding
Short Description
alpha-amylase-like
Curator Summary
Predicted to be secreted protein based on signalP prediction. Involved in starch mobilization. Mutants are defective in alpha-amylase activity. (Note: AMY1 has been found in the literature to be referred to as AMY3, which is not to be confused with AMY3/At1g69830).
Computational Description
alpha-amylase-like (AMY1); FUNCTIONS IN: alpha-amylase activity; INVOLVED IN: response to gibberellin stimulus, response to abscisic acid stimulus; LOCATED IN: extracellular region, apoplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 13 (InterPro:IPR006046), Alpha-amylase, plant (InterPro:IPR013775), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047), Alpha-amylase, C-terminal beta-sheet (InterPro:IPR012850); BEST Arabidopsis thaliana protein match is: alpha-amylase-like 2 (TAIR:AT1G76130.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G25010.1

Model type
Protein coding
Short Description
Nodulin MtN3 family protein
Computational Description
Nodulin MtN3 family protein; LOCATED IN: endomembrane system, integral to membrane, membrane; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: MtN3/saliva-related transmembrane protein, conserved region (InterPro:IPR018169), RAG1-activating protein 1 homologue (InterPro:IPR018179), RAG1-activating protein-1-related (InterPro:IPR004316); BEST Arabidopsis thaliana protein match is: Nodulin MtN3 family protein (TAIR:AT5G50800.1); Has 994 Blast hits to 947 proteins in 115 species: Archae - 0; Bacteria - 1; Metazoa - 233; Fungi - 0; Plants - 619; Viruses - 0; Other Eukaryotes - 141 (source: NCBI BLink).
Link
InterPro Scan - TAIR