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Mutant Lines
52-0780-1

52-0780-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 5; Position: 5484784 [Map]; Strand: w
  - AT5G16710.1 - [+] show detail - dehydroascorbate reductase 1
    Distance: 0; Region: intron5 (CDS)
  - AT5G16715.1 - [+] show detail - ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP...
    Distance: -569; Region: promoter
Flanking Genome Sequence: 52-0780-1 - 5-prime edge
```
ATAAACTAAC AAAATCGGTT ATACGATAAC GGTCGGTACG GGATTTTCCC 
ATCCTACTTT CATCCCGACA TTCTTTTTTG GATTTATTTT CATTCGGTTA 
AACCAACTGC ACCATAATGA TTTACAGAAT GTTTTCTCGA GGGAATCATT 
CACGAACACA CGGGCGGAAA CAGAGGATGT AATTGCTGGT TGGAGACCAA 
AGGTTATGGG TTAAGAGATT TTGATGTTGA CCTCAAGCT
```

3 prime edge [+] show sequence

Chromosome: 5; Position: 5484707 [Map]; Strand: w
- AT5G16710.1 - [+] show detail - dehydroascorbate reductase 1
  Distance: 0; Region: exon5 (CDS)
- AT5G16715.1 - [+] show detail - ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP...
  Distance: -646; Region: promoter

AAAGGACTAG CTCGGTTTTT CTGACATTTA TTTACCTCCA TGTAGGATTT 
GACGAAAGGA AGTGAATCTG GAACAGACCA GTTCTTGTAA TGTCCCAATG 
CAATCTTCAT GTGGTATAAC TTTGGTGCCA AGGACAAATC TGCTGCCGAG 
ATCTTCTCTC CATTTATGAA AGGGCCCTTC ACATTATGGA AGATAGAGAA 
GTAAATATAT GTTTCAATCA ACAGCCAGAA TAATCTACTC TAAGATCATC 
ACAACTATTA CTTAAGGATA TACAAGAGAT CAAAACCAAA AGAGCTTACA 
TTGTCCTTGA TATAATCGTT GAATGTAGTA AGCTCATCCA ACAAAACTTG 
CTCAGTTCCA TCTCCTGAAT CTCTGCTACT CCCCTAAAAA

AT5G16710.1

Model type: Protein coding
Short Description: dehydroascorbate reductase 1
Curator Summary: The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
Computational Description: dehydroascorbate reductase 1 (DHAR3); FUNCTIONS IN: glutathione dehydrogenase (ascorbate) activity; INVOLVED IN: protein amino acid glutathionylation; LOCATED IN: chloroplast stroma, chloroplast, chloroplast envelope; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Glutathione S-transferase, C-terminal-like (InterPro:IPR010987), Glutathione S-transferase/chloride channel, C-terminal (InterPro:IPR017933), Glutathione S-transferase, N-terminal (InterPro:IPR004045), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: dehydroascorbate reductase 2 (TAIR:AT1G75270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G16715.1

Model type: Protein coding
Short Description: ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases
Computational Description: embryo defective 2247 (EMB2247); FUNCTIONS IN: valine-tRNA ligase activity, nucleotide binding, aminoacyl-tRNA ligase activity, ATP binding; INVOLVED IN: tRNA aminoacylation for protein translation, embryo development ending in seed dormancy; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA-binding arm (InterPro:IPR010978), Valyl-tRNA synthetase, class Ia (InterPro:IPR002303), Aminoacyl-tRNA synthetase, class I, conserved site (InterPro:IPR001412), Valyl-tRNA synthetase, class Ia, tRNA-binding arm (InterPro:IPR019499), Aminoacyl-tRNA synthetase, class 1a, anticodon-binding (InterPro:IPR009080), Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Valyl/Leucyl/Isoleucyl-tRNA synthetase, class I, anticodon-binding (InterPro:IPR013155), Valyl/Leucyl/Isoleucyl-tRNA synthetase, class Ia, editing (InterPro:IPR009008), Aminoacyl-tRNA synthetase, class Ia (InterPro:IPR002300), Valyl-tRNA synthetase, class Ia, N-terminal (InterPro:IPR019754); BEST Arabidopsis thaliana protein match is: valyl-tRNA synthetase / valine--tRNA ligase (VALRS) (TAIR:AT1G14610.1); Has 40467 Blast hits to 37894 proteins in 3157 species: Archae - 953; Bacteria - 21019; Metazoa - 839; Fungi - 772; Plants - 400; Viruses - 0; Other Eukaryotes - 16484 (source: NCBI BLink).
Link: InterPro Scan - TAIR