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Mutant Lines
52-0894-1

52-0894-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 5; Position: 7533600 [Map]; Strand: c
  - AT5G22640.1 - [+] show detail - MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
    Distance: 0; Region: exon11 (CDS)
  - AT5G22640.2 - [+] show detail - MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
    Distance: 0; Region: exon12 (CDS)
  - AT5G22650.1 - [+] show detail - histone deacetylase 2B
    Distance: -417; Region: promoter
  - AT5G22650.2 - [+] show detail - histone deacetylase 2B
    Distance: -417; Region: promoter
Flanking Genome Sequence: 52-0894-1 - 5-prime edge
```
GACGAGTTAA TGCTACAGAA GAAGAGAGAC TCAAGACACA GCAGGAGTCT 
CAGCAAACAA ATCAGAACAT TTTGTCTGAA TTTGCAGCGA CAAAACCACG 
CTCATGTCCC CAACCGGTGT TTCCCAGAGG CCGGAGTCTT TGAGGTATTC 
ACTGGAGCTG CTATCNGGAG GAGAAGAAGA TGATGATACG TTGCAAGAAA 
GTAGTCGTGA TAGAGACATT AACTGAGACT GAGATCTTGA GCTTCCATAG 
CTTCTCTGGA CACAACCTCG GTTTGCTACC TTTCCCATCT TCAATCTAGC 
ATTGTTACTC GACAATGGAG GAAAATGGAT TGAAGCTGAT GCGGTATCAG 
CACCCTTTGA TGATTCCNGT GTTCACTT
```
3 prime edge [+] show sequence
1. Chromosome: 5; Position: 7533726 [Map]; Strand: c
  - AT5G22640.1 - [+] show detail - MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
    Distance: 0; Region: exon11 (3UTR)
  - AT5G22640.2 - [+] show detail - MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
    Distance: 0; Region: exon12 (3UTR)
  - AT5G22650.1 - [+] show detail - histone deacetylase 2B
    Distance: -291; Region: promoter
  - AT5G22650.2 - [+] show detail - histone deacetylase 2B
    Distance: -291; Region: promoter
Flanking Genome Sequence: 52-0894-1 - 3-prime edge
```
TCTTATGCAC TTCGAGGCTT CTTTTTTTGG TGGACAAGAT CATTTGAATT 
TTTGTTGTTT TATCTAATAA ATNTTTTNCN GAAACTATTT AAACGAAAAA 
ACNGANNATN TATTTCNGTT TTATTTTTCA TNATCATATG ATTTANAGAA 
TGAGAATCGA ATAATCTCAG AATATTTAAG TTAATATTTC ATTTATCAAT 
TTAGAATCTT TACCAAATAT GCAAATAATT TTACATTAAC GCAAATATTC 
CAAAATAAAG GAAGCGTTAC GAAATTAAGG GCAAAACGAC GCCGTGTAGA 
CTCTCGAAGA ACCCTAAAGA GACTTCTTAA ACCTCATTCG ATTATAAAAT 
AGCCCCAAAC CCACTGCCTC TTTCACTGCA ATTTCTCTCT TTCGCTTCTA 
A
```

AT5G22640.1

Model type: Protein coding
Short Description: MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
Curator Summary: EMB1211 is a MORN (multiple membrane occupation and recognition nexus) motif containing protein involved in embryo development and chloroplast biogenesis.
Computational Description: embryo defective 1211 (emb1211); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: chloroplast organization, embryo development, embryo development ending in seed dormancy; LOCATED IN: chloroplast thylakoid membrane, chloroplast, chloroplast envelope; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: MORN motif (InterPro:IPR003409); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G22640.2

Model type: Protein coding
Short Description: MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein
Curator Summary: EMB1211 is a MORN (multiple membrane occupation and recognition nexus) motif containing protein involved in embryo development and chloroplast biogenesis.
Computational Description: embryo defective 1211 (emb1211); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: chloroplast organization, embryo development, embryo development ending in seed dormancy; LOCATED IN: chloroplast thylakoid membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: MORN motif (InterPro:IPR003409).
Link: InterPro Scan - TAIR

AT5G22650.1

Model type: Protein coding
Short Description: histone deacetylase 2B
Curator Summary: Encodes a member of a plant-specific class of histone deacetylases. Controls the development of adaxial/abaxial leaf polarity. Its mRNA is widely expressed in stems, leaves, flowers and young siliques. Plant lines expressing RNAi constructs directed against this gene showed a marked reduction in agrobacterium-mediated root transformation.
Computational Description: histone deacetylase 2B (HD2B); FUNCTIONS IN: histone deacetylase activity; INVOLVED IN: DNA mediated transformation, negative regulation of transcription, polarity specification of adaxial/abaxial axis; LOCATED IN: cytosol, nucleolus, cell wall, plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: histone deacetylase 3 (TAIR:AT3G44750.1); Has 67941 Blast hits to 34482 proteins in 1815 species: Archae - 206; Bacteria - 15660; Metazoa - 22478; Fungi - 9360; Plants - 3234; Viruses - 784; Other Eukaryotes - 16219 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT5G22650.2

Model type: Protein coding
Short Description: histone deacetylase 2B
Curator Summary: Encodes a member of a plant-specific class of histone deacetylases. Controls the development of adaxial/abaxial leaf polarity. Its mRNA is widely expressed in stems, leaves, flowers and young siliques. Plant lines expressing RNAi constructs directed against this gene showed a marked reduction in agrobacterium-mediated root transformation.
Computational Description: histone deacetylase 2B (HD2B); FUNCTIONS IN: histone deacetylase activity; INVOLVED IN: DNA mediated transformation, negative regulation of transcription, polarity specification of adaxial/abaxial axis; LOCATED IN: cytosol, nucleolus, cell wall, plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: histone deacetylase 3 (TAIR:AT3G44750.2); Has 64884 Blast hits to 33590 proteins in 1781 species: Archae - 205; Bacteria - 13636; Metazoa - 21760; Fungi - 9071; Plants - 3171; Viruses - 807; Other Eukaryotes - 16234 (source: NCBI BLink).
Link: InterPro Scan - TAIR