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53-2354-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3752635 [Map]; Strand: c
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: exon1 (CDS)
      • AT1G11200.1 - [+] show detail - Protein of unknown function (DUF300)
        Distance: -730; Region: promoter
    Flanking Genome Sequence: 53-2354-1 - 5-prime edge 
    CTTTGAAGAT ATGGCATCGG CTTTTAGATC ATCCACGAGG TTGATTCTTG 
    TATTAGGTAT ACTGATTTTG TGTTCGGTTT CTTCTGTCCG AAGCTGGAGC 
    AAAGAAGGTC ATATTCTTAC TTGTAGAATT GCTCAGGTNA TTAAGTTAAT 
    GATCTATTGT TTGAAGCAAC TATTTTGGTT ATTCTTGTCT TATATATGTA 
    TTAGTATAAC TA

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3752751 [Map]; Strand: c
      • AT1G11190.1 - [+] show detail - bifunctional nuclease i
        Distance: 0; Region: exon1 (5UTR)
      • AT1G11200.1 - [+] show detail - Protein of unknown function (DUF300)
        Distance: -614; Region: promoter
    Flanking Genome Sequence: 53-2354-1 - 3-prime edge 
    CTTCAAAGTT TGANACTTNT NTAATGNTAA ACTCTTTTGT NTAAAAATGT 
    CTTCTCTTGT CTNTAACAAC CTCATCGAAT TTGAAAAGAN AACACACAGA 
    GAGNTTATAA ATAGAGAGAC AAGAATGGAC TTACAAGTTA GGTTAACCGG 
    AAGCAACATA TATGATGTTG AGTTTGTGTG CAAGCAAGAC ATTGCGAGTC 
    TCATACGTTC CTCATAAAAG TTGACAAAAA GAACACCGAG TATAAACATA 
    AGATGAAAGA GTCGTCAATT TATTAGTTAA AGCACATTCC TTATACTCTT 
    ATAGTATGTA ACAGCATCAT CCATGTGTCA TGTGTGGCAT ATCTTACAAT 
    AATTTAATTA ACGCCTAATC TTGTCAAACA TTTGCATCTT CTTAACTTGT 
    TAT

AT1G11190.1

Model type
Protein coding
Short Description
bifunctional nuclease i
Curator Summary
Encodes a bifunctional nuclease that acts on both RNA and DNA involved in nucleic acid degradation to facilitate nucleotide and phosphate recovery during senescence. It has mismatch-specific endonuclease activity with wide recognition of single base mismatches as well as the ability to cleave indel types of mismatches (heteroduplexes with loops).
Computational Description
bifunctional nuclease i (BFN1); FUNCTIONS IN: T/G mismatch-specific endonuclease activity, endoribonuclease activity, producing 5'-phosphomonoesters, single-stranded DNA specific endodeoxyribonuclease activity, nucleic acid binding; INVOLVED IN: DNA catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Phospholipase C/P1 nuclease, core (InterPro:IPR008947), S1/P1 nuclease (InterPro:IPR003154); BEST Arabidopsis thaliana protein match is: endonuclease 4 (TAIR:AT4G21585.1); Has 701 Blast hits to 698 proteins in 200 species: Archae - 0; Bacteria - 187; Metazoa - 0; Fungi - 98; Plants - 121; Viruses - 4; Other Eukaryotes - 291 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G11200.1

Model type
Protein coding
Short Description
Protein of unknown function (DUF300)
Computational Description
Protein of unknown function (DUF300); INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF300 (InterPro:IPR005178); BEST Arabidopsis thaliana protein match is: Protein of unknown function (DUF300) (TAIR:AT4G21570.1); Has 839 Blast hits to 837 proteins in 192 species: Archae - 0; Bacteria - 0; Metazoa - 285; Fungi - 195; Plants - 241; Viruses - 0; Other Eukaryotes - 118 (source: NCBI BLink).
Link
InterPro Scan - TAIR