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53-2381-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24338361 [Map]; Strand: c
      • AT5G60540.1 - [+] show detail - pyridoxine biosynthesis 2
        Distance: 0; Region: intron1 (CDS)
      • AT5G60548.1 - [+] show detail - conserved peptide upstream open reading frame 62
        Distance: -868; Region: promoter
      • AT5G60550.1 - [+] show detail - geminivirus rep interacting kinase 2
        Distance: -868; Region: promoter
    Flanking Genome Sequence: 53-2381-1 - 5-prime edge 
    CGCCGAGGAC CCCACTTGAA GAAACTAGTT AAAGGGTTCC TTTTTTATGT 
    GTGTTTTGAC ACGAATCTGG CGTTATTCAC CAAAATTCTC CAGAATTTCA 
    GTCAAAACGT CTCTTATACC TGAATAATTC AGTTAGTACT GTTGAAAGTA 
    ATTAGCTTTG AGATTGAAAA TGAAGGAACT TTGGGATTAG TTAGCCTTCA 
    AGTACATGAA GTAATGTTTT GTATCTGAAA CCTTTAAACA TTGTGGACCA 
    AACTACTCGT TGATCTTATG GATGATATTT TCTGTCTTTA ATGGAAGCTG 
    TGATAAGTGT AGGAAAGGGA GAGCTATCAT TTACTTTTAT CTTTACCAAA 
    CTCCTTGTTT AGATTTGGTT GTTTTTACAG TTTTAGTGGT GAGGACGAAG 
    AA

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24338473 [Map]; Strand: c
      • AT5G60540.1 - [+] show detail - pyridoxine biosynthesis 2
        Distance: 0; Region: exon1 (CDS)
      • AT5G60548.1 - [+] show detail - conserved peptide upstream open reading frame 62
        Distance: -756; Region: promoter
      • AT5G60550.1 - [+] show detail - geminivirus rep interacting kinase 2
        Distance: -756; Region: promoter
    Flanking Genome Sequence: 53-2381-1 - 3-prime edge 
    ACTCCTCGCA AACAATAACT TTAGATTCTA GAGAGAGAGG AGCGGACCAA 
    GTTATGATAC TCGGCGAGTT TGGCCATGGT GGTGCTCTCG CCGCCAGGAA 
    TGATAAGAGA AGAAACGGTG AGAAGCTGGT CAGCCTTCCT AATCTCGACG 
    CCTTGGACAC CGAGCCGCCG CAGAGCCGCG ATGTGCTCAT TGAAAGAACC 
    TTGCAAAGCT AAAACTCCGA CGGTCATTGC GGCGGCGACG GTCAAAGAAA 
    GAGAGTGTCA GATATTATTA AACACTAAAA CTTTGTGAAT GGAATTGGGA 
    CACTCACAAA AGCCTTTTTG TAACAACCAT CGACGATTTT GTTTTGTGTT 
    TCTTTCTTTG CCCACGATTT GGATTTCTAT TCAGATATTT TCTTATAATA 
    ACATTTTTAA ATATAAAGAT ATCACGGTAT TAATAACTAT TGTGAACAAA 
    TTCAATTAAA AAGCCATAAA CAATTATGCT AACAAAACAA AAATATACAC 
    TTATTAAGCT TTTATGTGAT TATAATTCAA ATTCTCTAGG AAAAATTGTA 
    ATCTATTTTA CAAAATTTTA AAGATGATGA TATAATATTA TATA

AT5G60540.1

Model type
Protein coding
Short Description
pyridoxine biosynthesis 2
Curator Summary
Encodes a protein predicted to function in tandem with PDX1 to form glutamine amidotransferase complex with involved in vitamin B6 biosynthesis. PDX2 is predicted to function as glutaminase within the complex.
Computational Description
pyridoxine biosynthesis 2 (PDX2); CONTAINS InterPro DOMAIN/s: PdxT/SNO family, conserved site (InterPro:IPR021196), SNO glutamine amidotransferase (InterPro:IPR002161); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60548.1

Model type
Protein coding
Short Description
conserved peptide upstream open reading frame 62
Curator Summary
Upstream open reading frames (uORFs) are small open reading frames found in the 5' UTR of a mature mRNA, and can potentially mediate translational regulation of the largest, or major, ORF (mORF). CPuORF62 represents a conserved upstream opening reading frame relative to major ORF AT5G60550.1
Computational Description
conserved peptide upstream open reading frame 62 (CPuORF62); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60550.1

Model type
Protein coding
Short Description
geminivirus rep interacting kinase 2
Curator Summary
Encodes a geminivirus Rep interacting kinase (GRIK; GRIK1/AT3G45240, GRIK2/AT5G60550). GRIKs are SnRK1 (SNF1-related kinases) activating kinases. Both GRIKs specifically bind to the SnRK1 catalytic subunit and phosphorylate the equivalent threonine residue in its activation loop in vitro.
Computational Description
geminivirus rep interacting kinase 2 (GRIK2); CONTAINS InterPro DOMAIN/s: Calcium/calmodulin-dependent protein kinase kinase (InterPro:IPR020657), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: geminivirus rep interacting kinase 1 (TAIR:AT3G45240.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR