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53-4279-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24201159 [Map]; Strand: w
      • AT5G60100.2 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: exon1 (5UTR)
      • AT5G60100.3 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: exon1 (5UTR)
      • AT5G60100.1 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: exon1 (5UTR)
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: -795; Region: promoter
    Flanking Genome Sequence: 53-4279-1 - 5-prime edge
    GGGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCTG ACCACCACCA GAATCTCTCA CCATATCTGA
    CTTGAAAAAC CAAATTACAA CCAAAAAAGA ACAGATTTTT GCTTAATCTG
    ATTTGCTTAG CATTTAATCA CCTAATCATT CAAAAATGTT TTCATTCGTA
    TCTATTTTTC TTTTACCAAC AACTTTACAG AACCAAATTG CTACTAAAAT
    AGAATAAAAA GAAAGAAAAT TTAAAAAACT TGATAGAGAA TCAGAGGAAG
    AGAAGATTGG GATTTTTTGG GAGGTGGTAG AACAAAAAGG TCAGAGAAGA
    TATTATTTTG GAGTTTGATG ACGTGGCAGA ACTAACCTAC GTGTCAAGTA
    GGACCAGATA AATTTACGCA TGCTGAGATT AGTGACATGG ACAAAATCAA
    AAGCT
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 24201113 [Map]; Strand: w
      • AT5G60100.2 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.3 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60100.1 - [+] show detail - pseudo-response regulator 3
        Distance: 0; Region: intron1 (5UTR)
      • AT5G60110.1 - [+] show detail - pumilio 18
        Distance: -841; Region: promoter
    Flanking Genome Sequence: 53-4279-1 - 3-prime edge
    GTGGTGGTGG TGTTCATCAA TGGATTATGT GAAAAAGGTT TGCTTTTGTT 
    TGTCTTGTCG CTTTTACAAT CTGCTGCTGT TTGAATTTTG TGCTTATTTC
    ATTAAGTATT ATAGCTTAGA GCCTTAGAGT TTGATTTTGT ATCTGGGTTC
    TGTAGAGTTT ATAAGAAATT TGAGAAAGTA GGAGATCTTT ACAATCATCT
    AATGAATAAA AGAAACTGTG TTCAAAGTTT GTTAACTACT TACCAAGTAC
    TGTGATAATT TTCCATTGGA ACATTGATAG CTTTTGATTT TAGAAACAAC
    AAGTTTCAGA TTGTTTTCTA AAGCTGAGTG TGTGCTACTA TTGAAATTTG
    ATTTGGCTTG TTTAGTTTTT CTTTGGACAA GTTTGATGAT TTATCCAAAT
    TCAGTTTTTT ATTTTTTGGT TCTGGTGTGA GACTGTGATC TTCTTACGGA
    ATGTTGCTTT TTTTTTNGTT NCTCGTTCCT NATNGGGCTG AGGTTTTGGT
    GAAGGGATTA AAATAANTTT GGGGAAANAA AAAATGTGCA AGTGTTNTGT
    CANNGTAAAG GGGAAAGTTT NTGCAAATCT TANCTGTNCC TTGANGCTGN
    TTNTATTNTT TCTTGTNATG GGGGCNGGNC TGACCCTTTN TNAAAATTAN
    NTTTAAAAAN GNGTTTAATA CNTTGAACCG GNGANAANNG NAACCNAAGG
    NNNGNGTTNG GTCNTTTNAA AAAAAAATTN NANTG

AT5G60100.1

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G60100.2

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1).
Link
InterPro Scan - TAIR

AT5G60100.3

Model type
Protein coding
Short Description
pseudo-response regulator 3
Curator Summary
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
Computational Description
pseudo-response regulator 3 (PRR3); CONTAINS InterPro DOMAIN/s: CheY-like (InterPro:IPR011006), Signal transduction response regulator, receiver domain (InterPro:IPR001789), CCT domain (InterPro:IPR010402); BEST Arabidopsis thaliana protein match is: pseudo-response regulator 7 (TAIR:AT5G02810.1).
Link
InterPro Scan - TAIR

AT5G60110.1

Model type
Protein coding
Short Description
pumilio 18
Curator Summary
Encodes a member of the Arabidopsis Pumilio (APUM) proteins containing PUF domain (eight repeats of approximately 36 amino acids each). PUF proteins regulate both mRNA stability and translation through sequence-specific binding to the 3' UTR of target mRNA transcripts.
Computational Description
pumilio 18 (PUM18); FUNCTIONS IN: RNA binding, binding; LOCATED IN: cytoplasm; CONTAINS InterPro DOMAIN/s: Pumilio RNA-binding repeat (InterPro:IPR001313), Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: pumilio 19 (TAIR:AT5G60180.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR