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Mutant Lines
54-2266-1

54-2266-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 3; Position: 2871307 [Map]; Strand: c
- AT3G09340.1 - [+] show detail - Transmembrane amino acid transporter family protein
  Distance: -781; Region: promoter
- AT3G09350.1 - [+] show detail - Fes1A
  Distance: 0; Region: intron1 (CDS)
- AT3G09350.2 - [+] show detail - Fes1A
  Distance: 0; Region: intron1 (5UTR)
- AT3G09350.3 - [+] show detail - Fes1A
  Distance: 0; Region: intron1 (5UTR)

CCACCAAGCA TCCCATCAGT TTCAGACAAA TCAAAAGACA GAACCTAAAA 
AACCAACTAC AAGATGAATG TCCGTACCTC AACTGGCGAG TGGGTCGAGT 
ACCGTCGGCG TGAGAGAGAC TCCACTTAAG CAAACCATCC CAATTAGGTC 
CGTCTTTCGC CATTGAAGAA TTACTTTCAC AGTGAAAAGA ACTTGTAACA 
GAAGAAGAAA TTACAAAACT CGAAGCCTTT TCGTCTTTGT AATAATAAAG 
AGGAAGAGTC GACTAATCGC TAGTTTGGTT TTATAGAAGA CCTGAGATTA 
TTCGCGAAGC TACTACAACA TTCTTAAAAA TCTTAAACCT CGTCGACGAC 
CTCTCAAGGG AAAAGGAAAA GCGAAGACGA TGACGCCTCC TCCACAAATT 
GGGCCTTTAA GACTCAAGCC CAAATAGTTC AGTATTATTG GGCTTAAATT 
AGCTCGATAA GCCTTTCTTG AATTTACCTT TTTTCTTTAA TTTATTGGCA 
AATCTATGCC ACGCATGTAC CTTTTGACTT TGGACCTTTT AAACATGTGA 
ACGTTACCTT CCACTCTAAA TTTAACGACC AGTCGACCAG AATTTAATTC 
GTTACATATG ATCAAGTAGT ATCAATGGAC TAATACAAAG TGTTTTTTTT 
CCTAAGACTA ATACAAAGTT GACAGACGTC AGACGTGACA CCN

3 prime edge [+] show sequence
1. Chromosome: 3; Position: 2871423 [Map]; Strand: c
  - AT3G09340.1 - [+] show detail - Transmembrane amino acid transporter family protein
    Distance: -897; Region: promoter
  - AT3G09350.1 - [+] show detail - Fes1A
    Distance: 0; Region: intron1 (CDS)
  - AT3G09350.2 - [+] show detail - Fes1A
    Distance: 0; Region: intron1 (5UTR)
  - AT3G09350.3 - [+] show detail - Fes1A
    Distance: 0; Region: exon2 (5UTR)
Flanking Genome Sequence: 54-2266-1 - 3-prime edge
```
CTTGGTGGGT AGTTTAGGTA TCTGTGTGGC TATGATTGAG CTTTCACTTG 
AGGAGATGAA TCTTATGAGT GAATT
```

AT3G09340.1

Model type: Protein coding
Short Description: Transmembrane amino acid transporter family protein
Computational Description: Transmembrane amino acid transporter family protein; CONTAINS InterPro DOMAIN/s: Amino acid transporter, transmembrane (InterPro:IPR013057); BEST Arabidopsis thaliana protein match is: Transmembrane amino acid transporter family protein (TAIR:AT3G09330.1); Has 5037 Blast hits to 4989 proteins in 303 species: Archae - 8; Bacteria - 84; Metazoa - 1819; Fungi - 980; Plants - 1422; Viruses - 9; Other Eukaryotes - 715 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G09350.1

Model type: Protein coding
Short Description: Fes1A
Curator Summary: Encodes one of the Arabidopsis orthologs of the human Hsp70-binding protein 1 (HspBP-1) and yeast Fes1p: Fes1A (AT3G09350), Fes1B (AT3G53800), Fes1C (AT5G02150). Fes1A is cytosolic and associates with cytosolic Hsp70. Mutants showed increased heat-sensitive phenotype suggestion the involvement of Fes1A in acquired thermotolerance. Does not have nucleotide exchange factor activity in vitro.
Computational Description: Fes1A (Fes1A); CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: Fes1C (TAIR:AT5G02150.1); Has 622 Blast hits to 617 proteins in 204 species: Archae - 0; Bacteria - 0; Metazoa - 202; Fungi - 158; Plants - 182; Viruses - 0; Other Eukaryotes - 80 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G09350.2

Model type: Protein coding
Short Description: Fes1A
Curator Summary: Encodes one of the Arabidopsis orthologs of the human Hsp70-binding protein 1 (HspBP-1) and yeast Fes1p: Fes1A (AT3G09350), Fes1B (AT3G53800), Fes1C (AT5G02150). Fes1A is cytosolic and associates with cytosolic Hsp70. Mutants showed increased heat-sensitive phenotype suggestion the involvement of Fes1A in acquired thermotolerance. Does not have nucleotide exchange factor activity in vitro.
Computational Description: Fes1A (Fes1A); CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: Fes1B (TAIR:AT3G53800.1); Has 594 Blast hits to 590 proteins in 194 species: Archae - 0; Bacteria - 0; Metazoa - 194; Fungi - 149; Plants - 182; Viruses - 0; Other Eukaryotes - 69 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G09350.3

Model type: Protein coding
Short Description: Fes1A
Curator Summary: Encodes one of the Arabidopsis orthologs of the human Hsp70-binding protein 1 (HspBP-1) and yeast Fes1p: Fes1A (AT3G09350), Fes1B (AT3G53800), Fes1C (AT5G02150). Fes1A is cytosolic and associates with cytosolic Hsp70. Mutants showed increased heat-sensitive phenotype suggestion the involvement of Fes1A in acquired thermotolerance. Does not have nucleotide exchange factor activity in vitro.
Computational Description: Fes1A (Fes1A); CONTAINS InterPro DOMAIN/s: Armadillo-like helical (InterPro:IPR011989), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: Fes1B (TAIR:AT3G53800.1); Has 594 Blast hits to 590 proteins in 194 species: Archae - 0; Bacteria - 0; Metazoa - 194; Fungi - 149; Plants - 182; Viruses - 0; Other Eukaryotes - 69 (source: NCBI BLink).
Link: InterPro Scan - TAIR