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Mutant Lines
54-2670-1

54-2670-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 3; Position: 23385285 [Map]; Strand: c
- AT3G63300.1 - [+] show detail - FORKED 1
  Distance: 0; Region: exon1 (CDS)
- AT3G63300.2 - [+] show detail - FORKED 1
  Distance: 0; Region: exon1 (5UTR)

CTGTGAAGTG GCCGAGGCAG CGAACGAGAA CTGGCTACTA ACACTAGTAC 
TGTTCTCCGG CGGACATTCT TCTTTCTCAG GGTTTAGCAG TTTCTTCCGG 
TATCGGAGTG TTAATGGATG AGGGAGGCCT GTTGGTGGCT GAAGCTGATT 
TGGCGGTGTG GAGAGCTCTG GAGACTTCCA AGGCAGAGAC ACTCCAGGAT 
CTAGACAAGA ACTCCATTGG GCCTCGTGGG CTTTCCGGTA GTTGAGTACT 
ATTACCGCCT GAAGAAAATA TCTCCGGCCG GCGAGAGCTG TTGATTGGGT 
TCGGTCGTCT GAGTTCTCTC TCCATCCTTT CTCTCTCTCT CTAATTAACT 
TTGCTTCGAC TTCAGAGCTT GACTAGGAAA CTTATGTGTG TGTGTATATA 
TAGATAGACA AAGTAACGTT TAGGTATTTC ACCTTTTCTT TTTCTTTTAA 
AGAAAAATGT GATTATATTT CGATGAGAGT AGGAGTGAGT AGTACTACTC 
TTGACATAAT ATATAGTCGT TGCATAAATA ATGGATTACT ACTATAAAAA 
AAAAANACGT CNCTTTTNAA TAATAAGGCC GCNTNTTTGC CAATCCCNTT 
GTTTNTTACA CATAAATNTT ACTTTAAATG TTGTNTCANC NANTCTTGAA 
AAAAGGAGGG AATNAAGNCN TCCTGNNTTT GCCTTTTNTT ATTTAATCAA 
AACT

3 prime edge [+] show sequence
1. Chromosome: 3; Position: 23385401 [Map]; Strand: c
  - AT3G63300.1 - [+] show detail - FORKED 1
    Distance: 0; Region: intron1 (CDS)
  - AT3G63300.2 - [+] show detail - FORKED 1
    Distance: 0; Region: exon1 (5UTR)
Flanking Genome Sequence: 54-2670-1 - 3-prime edge
```
CTTCACAGCT TGTTCTTGAG CGCATAATGT CTCAATCGGT ACGTTTACAT 
AGCTATGGAC CGACCGACAC ACACTGTTTT TGTATCTTGA TGATGAGTTT 
ATTCTGTTTT GAAAAATGAA GGAGGTGTCA CCGTTAACGT CGGGGAGGCT 
ATCACACAGC AGCGGCCCAC TCAACGGCGG CGGTTCTTTT ACGGAGACAG 
ACAGTCCTCC CATCTCCCCT TCCGATGAAT T
```

AT3G63300.1

Model type: Protein coding
Short Description: FORKED 1
Curator Summary: Encodes a pleckstrin homology domain- and DUF828-containing protein. Mutants have defects in leaf vascular pattering, with vascular bundles that fail to meet distally in both the cotyledons and leaves. Necessary to the formation of the closed leaf vascular pattern characteristic of dicot leaves in response to auxin. Redundant with FKD2. FKD1 may influence PIN1 localization in an auxin dependent manner. proposed to be a key component of the auxin canalization pathway.
Computational Description: FORKED 1 (FKD1); CONTAINS InterPro DOMAIN/s: Pleckstrin-like, plant (InterPro:IPR013666), Protein of unknown function DUF828 (InterPro:IPR008546); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region (TAIR:AT3G22810.1); Has 742 Blast hits to 348 proteins in 78 species: Archae - 0; Bacteria - 118; Metazoa - 127; Fungi - 51; Plants - 226; Viruses - 0; Other Eukaryotes - 220 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G63300.2

Model type: Protein coding
Short Description: FORKED 1
Curator Summary: Encodes a pleckstrin homology domain- and DUF828-containing protein. Mutants have defects in leaf vascular pattering, with vascular bundles that fail to meet distally in both the cotyledons and leaves. Necessary to the formation of the closed leaf vascular pattern characteristic of dicot leaves in response to auxin. Redundant with FKD2. FKD1 may influence PIN1 localization in an auxin dependent manner. proposed to be a key component of the auxin canalization pathway.
Computational Description: FORKED 1 (FKD1); CONTAINS InterPro DOMAIN/s: Pleckstrin-like, plant (InterPro:IPR013666), Protein of unknown function DUF828 (InterPro:IPR008546); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF828) with plant pleckstrin homology-like region (TAIR:AT3G22810.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR