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54-4725-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 26628012 [Map]; Strand: c
      • AT5G66690.1 - [+] show detail - UDP-Glycosyltransferase superfamily protein
        Distance: 1219; Region: intergenic
      • AT5G66700.1 - [+] show detail - homeobox 53
        Distance: -6308; Region: intergenic
    Flanking Genome Sequence: 54-4725-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCC 
    TCCGTAAAGG AACGTATGTG TTTTCTACAT CCTTACAAAT ATGTTTACAT 
    GATTTCTTTC CCAATTACAA TTAGGCTTTG AGAGCCGGTA ATAATTCCAT 
    ATTTCAAGTT TTTTTCTTAT TCAAGTCATT TTTCCGTGGG CNTTAACCAA 
    ACTGTCCATA TTTGGGCCCA ATTGCTGTTA TCATACGAAG ATCTGATCAT 
    ACATTCATAC TGTACAAAAA TTAACCTCTA TTAGAGATTG ATACGCCGAC 
    TCTGTATTAA GAAAAGCACT TATCGATCGT TACATAATCT ACAGCGCGAT 
    ATAAATTTTG ACAGCATGTG CTAGACCTAC TTTTTTTTGT TGTTGTTGTG 
    TGGGCTATAC CTAAAAATAT TAGCAGTATC GGACCGACAT TATCAAATAA 
    AGATATACAC GTAGAACTCA AATAAAGCCC ACTCCAGTGT TGTTGGATCT 
    ACAACAGCTT CATTTTATCC GAAAGATCTA AAACAGCTTT ATGTGTGCTC 
    TAAAGATAAT AGAGACAAAT TTCGCCGCCA TAGCACGACA ATTGGGTTTA 
    CATTATCGCT AAGTAAAGGA ATCTGTTATA AATAAGAACT AATTAAAATC 
    GTTTTGTGAG TTTATGATTA ACACCAGTTG GTTTCATGTG CTTAAAGTTG 
    GTCCATGTAC CATATTTTTG TAATTTTGTA CGAGAATACA GCTCG

AT5G66690.1

Model type
Protein coding
Short Description
UDP-Glycosyltransferase superfamily protein
Curator Summary
UGT72E2 is an UDPG:coniferyl alcohol glucosyltransferase which glucosylates sinapyl- and coniferyl aldehydes as well as sinapyl- and coniferyl alcohol. The enzyme is thought to be involved in lignin metabolism. A knockdown mutant line (72E2KD) was obtained using RNAi silencing. A twofold reduction in coniferyl alcohol 4-O-glucoside and sinapyl alcohol 4-O-glucoside was detected in this line compared to wildtype. In comparison, both knockout and knockdown lines of UGT72E1 and UGT72E3, respectively, failed to display the same reduction in phenylpropanoid 4-O-glucosides.
Computational Description
UGT72E2; CONTAINS InterPro DOMAIN/s: UDP-glucuronosyl/UDP-glucosyltransferase (InterPro:IPR002213); BEST Arabidopsis thaliana protein match is: UDP-Glycosyltransferase superfamily protein (TAIR:AT5G26310.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G66700.1

Model type
Protein coding
Short Description
homeobox 53
Curator Summary
Encodes a homeodomain protein. Member of HD-ZIP 1 family, most closely related to HB5. AtHB53 is auxin-inducible and its induction is inhibited by cytokinin, especially in roots therefore may be involved in root development.
Computational Description
homeobox 53 (HB53); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: response to auxin stimulus, regulation of transcription, DNA-dependent, root development; LOCATED IN: nucleus; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Homeobox, conserved site (InterPro:IPR017970), Homeobox (InterPro:IPR001356), Homeodomain-like (InterPro:IPR009057), Helix-turn-helix motif, lambda-like repressor (InterPro:IPR000047), Homeodomain-related (InterPro:IPR012287); BEST Arabidopsis thaliana protein match is: homeobox protein 40 (TAIR:AT4G36740.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR