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ET13216

CSHL Arabidopsis genetrap mutant lines

Original: ET13216

Phenotypes

Morphological phenotypes

Original description

  • semi-sterile (unconfirmed)
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: ET13216

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 2688789 [Map]; Strand: c
      • AT1G08490.1 - [+] show detail - chloroplastic NIFS-like cysteine desulfurase
        Distance: -194; Region: promoter
      • AT1G08500.1 - [+] show detail - early nodulin-like protein 18
        Distance: -227; Region: promoter
    Flanking Genome Sequence: ET13216 - 3-prime edge 
    GTAGAGGTAT TTTACCGACC GTTACCGACC GTTTTCATCC CTAAGTTGAC 
    AGATATTTAT GAAAGAAGTA ATGATACGTA GGGAAGCGAA GACAGCAAAA 
    GTAAGGTGTG AGAGTGAGAG ATCACATGGG GAAGAGCAAA GAGGCGTTGT 
    CATTTGATTG GACCGACCAC TTCGTATCGT CTCATGTTCC TTCGCATCCC 
    CAAGAATTGT CCACATGTCA CACTCACACA TGTCTTGGTT TTATATGCAG 
    TCAATGACGA GCTCATTATT ACTTTATGTA CAGTTTCTTA TGCCTTTCTT 
    TATTACAAAG AAAGATCAAT CAACGAATCA CAATTTATAA TCAAGAAAAA 
    CGGACAAGAG AGTAAGAAGA GTTATGTCGC CTTCTTGTTC ATCTTGTGTC 
    AATGTGTTAC TCATCATGTG CCTTATGCTT CTGAGTTTAT CAGCTGATGC 
    GTACAAGAAC TACACTGTGG GAGAGTCCAC GGGATGGTTC GATATTCAAG 
    AGAGACCCTC TGCTAATTAT CAGAAATGGG CCGATTCCAA ATCCTTTTCT 
    TTGGGTGATT TCCTCAGTAA GATTTTTTTC TTCAACCCCA TTTTCGTTAC 
    TACAATAACT TAGCAAAATG TTATATCGGT TGTTTTGTTT TCAATATG

  • 5 prime edge [+] show sequence

    Not mapped

    Flanking Genome Sequence: ET13216 - 5-prime edge 
    TGGTCCCCGC CTTTTTTGGT TAAAACCTTT AAACCGCCCC AGATTATAAA 
    CCATAGTCGT TGGAGTAAAT TTATCCCATG GCTGCACAAA CACATTTTTG 
    CAGGTTTAAA ATTGTTGAAT TGTCCACTAG ACCACACCAT TTAACCAATA 
    ATTGGACCAG AAGAGCATGT TGGGATACTT TTTTCAAAAT TTATGTGGGT 
    ATTAACTATT ATGTCCTTTA ATCCCCCGCA TATCCAGGAG TAACGTATTA 
    TTAATATTTA TATGTTGGCT AAATTGTCCC ATTTATGCTT AAAAGTCCTA 
    CACAACCTTC AAGGGCCTAA TATTTCATAA CAACCCTGTA ACTGACGTTG 
    TCCATATTTA TAATTTTTTA TTCGCCCCGA ATTGAAGCCT TGACAGGAAG 
    TTGCACCTTA GGTAAACGCT GCACTTATTG GAGAATTGTA GGAGACCCAA 
    ATTCTACCTA CGAAGAAAAT TCAGACGCTA TACCCTACAA TAATATAAAT 
    TAGCAGGAAA AAAATAGATG CCTTATGTTT TATATTATTT ACCGGAAGTT 
    TTGACCACGC AAATGCCAAA AAATAAAACG A

AT1G08490.1

Model type
Protein coding
Short Description
chloroplastic NIFS-like cysteine desulfurase
Curator Summary
Chloroplastic NifS-like protein that can catalyze the conversion of cysteine into alanine and elemental sulfur (S(0)) and of selenocysteine into alanine and elemental Se (Se(0)). Overexpression enhances selenium tolerance and accumulation.
Computational Description
chloroplastic NIFS-like cysteine desulfurase (CPNIFS); FUNCTIONS IN: transaminase activity, selenocysteine lyase activity, cysteine desulfurase activity; INVOLVED IN: iron incorporation into metallo-sulfur cluster, response to selenium ion, sulfur metabolic process, selenium metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Cysteine desulfurase, SufS (InterPro:IPR010970), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: nitrogen fixation S (NIFS)-like 1 (TAIR:AT5G65720.1); Has 22660 Blast hits to 22653 proteins in 2897 species: Archae - 350; Bacteria - 15408; Metazoa - 379; Fungi - 404; Plants - 268; Viruses - 1; Other Eukaryotes - 5850 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G08500.1

Model type
Protein coding
Short Description
early nodulin-like protein 18
Computational Description
early nodulin-like protein 18 (ENODL18); FUNCTIONS IN: electron carrier activity, copper ion binding; LOCATED IN: anchored to membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: LP.06 six leaves visible, 4 anthesis, petal differentiation and expansion stage, LP.08 eight leaves visible, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Plastocyanin-like (InterPro:IPR003245), Cupredoxin (InterPro:IPR008972); BEST Arabidopsis thaliana protein match is: Cupredoxin superfamily protein (TAIR:AT2G31050.1); Has 799 Blast hits to 788 proteins in 52 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 799; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
Link
InterPro Scan - TAIR