ET6517

CSHL Arabidopsis genetrap mutant lines

Phenotypes

Morphological phenotypes

Original description

small siliques, very few seed, semi-sterile

Anatomical entity /structure development stage	Phenotypes
fruit (PO:0009001)	decreased size (PATO:0000587)
seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997)

Original: ET6517

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

3 prime edge [+] show sequence

Chromosome: 1; Position: 28453948 [Map]; Strand: c
- AT1G75780.1 - [+] show detail - tubulin beta-1 chain
  Distance: -308; Region: promoter

GTAGAGGTAT TTTACCGACC GTTACCGACC GTTTTCATCC CTAATTATAT 
CGTCCATTTA ACTATTTAAG TGTGATAGCT TTCAAGTATC GATCATTCAT 
TCTAGTTTCA AATCTTGATC CATTTGATGA ATCTTTTACT ATTCGAATTT 
CATCACGACT AGCTACAACC ACCCTTGATT ATATGTTATG ACTTCTGAAA 
ACATCGAGAA ACAAAAATTC AATAATAGTG CGTAGAAGAA GATGATCAAT 
GCGTAATGAA TACAGTAACT TTGCATGTAG TATCTTAGTT GTTTTAGTGA 
CATAGCACAT GTAGCAAGAC GTAATAAACG TTCTGGAGAT CTTGAATGCA 
TATGTACCTA TTTCTATGTA AACGATAAAA CACAATAATT GAAATCGGTT 
GAATGATCAA AAGTATAGAA AAATAGCATC ACACTGATTG TGTTTGGATC 
TATTATACAA TATTGATGAT AACATGAAAA ACAAATAATG CCATAGCACA 
TGTAGCTAGG TAGATGTAGA CTGTAGTAAG ATCTGAGATT TGATCCAATT 
ATTTTTTCAT TTTA

5 prime edge [+] show sequence
1. Chromosome: 4; Position: 12839245 [Map]; Strand: c
  - AT4G24972.1 - [+] show detail - tapetum determinant 1
    Distance: 0; Region: intron3 (CDS)
Flanking Genome Sequence: ET6517 - 5-prime edge
```
CTTTTCTCCA ATTCTCTCCG GGTCTACACT CCTTTCTGGT TTTCCTTTAT 
ATAAACAATC ACACACACAA TCAAAAC
```
5 prime edge [+] show sequence
1. Chromosome: 4; Position: 17181257 [Map]; Strand: c
  - AT4G36360.1 - [+] show detail - beta-galactosidase 3
    Distance: 0; Region: exon1 (5UTR)
  - AT4G36360.2 - [+] show detail - beta-galactosidase 3
    Distance: 0; Region: exon1 (5UTR)
Flanking Genome Sequence: ET6517 - 5-prime edge
```
TCCTACTTTC TCCCTGGTGA TGATGCGAGT AAGAGACACT TGTGAAGTGC 
AATAAAACCC CCTTCTTCTT CCTCTCTTTC TCCTTCGTCT TCTTCTTCTT 
CTTCCTTCCT TCCTTCTCTC TCCTTATTAA GAAAGACCAA CACAGACACG 
GGAAGAAGTA GCTTGG
```
5 prime edge [+] show sequence
1. Chromosome: 1; Position: 28453642 [Map]; Strand: c
  - AT1G75780.1 - [+] show detail - tubulin beta-1 chain
    Distance: -2; Region: promoter
Flanking Genome Sequence: ET6517 - 5-prime edge
```
ACTTCCCAAA AAAAACTCTC CCGCCTCTCT CTCTCCCTCT CCCTTCTTAT 
TCTCCTCCT
```

5 prime edge [+] show sequence

Chromosome: 1; Position: 28453871 [Map]; Strand: c
- AT1G75780.1 - [+] show detail - tubulin beta-1 chain
  Distance: -231; Region: promoter

TAATTCAACA ATATTCTTAA ATAACATTAA TCATTCACTT TGTAGAAATT 
TCAATTTGAC GGTGCTAACA ACAATATGTG ACATTTTTGT TTGCAGCAAA 
AAAGTCAAAT GGTAAAAAAA AACATTTTTT TAAAAAGAGA GTATAGTAAA 
AAAACAGAGG ATAAAAAACG AACAAACGGT TAAAGCTGGC AAAGATCCCA 
TCTTCCCCAC GCTGTCAAAT TTTGTCACTA CTTCCCAAAA AAAACTCTCC 
CGCCTCTCTC TCTCCCTCTC CCTT

AT1G75780.1

Model type: Protein coding
Short Description: tubulin beta-1 chain
Curator Summary: beta tubulin gene downregulated by phytochrome A (phyA)-mediated far-red light high-irradiance and the phytochrome B (phyB)-mediated red light high-irradiance responses
Computational Description: tubulin beta-1 chain (TUB1); FUNCTIONS IN: structural molecule activity, GTP binding, GTPase activity; INVOLVED IN: response to light stimulus, unidimensional cell growth; LOCATED IN: vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Beta tubulin (InterPro:IPR002453), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Beta tubulin, autoregulation binding site (InterPro:IPR013838), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin beta-5 chain (TAIR:AT1G20010.1); Has 23671 Blast hits to 23575 proteins in 4891 species: Archae - 36; Bacteria - 42; Metazoa - 4470; Fungi - 14216; Plants - 1549; Viruses - 0; Other Eukaryotes - 3358 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G24972.1

Model type: Protein coding
Short Description: tapetum determinant 1
Curator Summary: Encodes a novel small protein which is similar to proteins of unknown function from other plant species. TPD1 is involved in cell specification during anther and pollen development. Identified in a screen for male steriles. Mutants lack tapetal cells and have an increased number of microsporocytes. Expressed in flower buds, leaves and young seedlings. In anthers, TPD1 is expressed throughout pollen development in parietal cells and sporocytes. Physically interacts with the LRR kinase EMS1 and that interaction results in phosphorylation of TPD1.
Computational Description: TAPETUM DETERMINANT 1 (TPD1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: cell fate determination, anther development; LOCATED IN: endomembrane system; EXPRESSED IN: microsporocyte, floral meristem, flower, anther, leaf; EXPRESSED DURING: seedling growth; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G32583.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G36360.1

Model type: Protein coding
Short Description: beta-galactosidase 3
Curator Summary: putative beta-galactosidase (BGAL3 gene)
Computational Description: beta-galactosidase 3 (BGAL3); FUNCTIONS IN: beta-galactosidase activity; INVOLVED IN: lactose catabolic process, using glucoside 3-dehydrogenase, carbohydrate metabolic process, lactose catabolic process via UDP-galactose, lactose catabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 35, conserved site (InterPro:IPR019801), Glycoside hydrolase, family 35 (InterPro:IPR001944), D-galactoside/L-rhamnose binding SUEL lectin (InterPro:IPR000922), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Galactose-binding domain-like (InterPro:IPR008979), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta galactosidase 1 (TAIR:AT3G13750.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G36360.2

Model type: Protein coding
Short Description: beta-galactosidase 3
Curator Summary: putative beta-galactosidase (BGAL3 gene)
Computational Description: beta-galactosidase 3 (BGAL3); FUNCTIONS IN: beta-galactosidase activity; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 35, conserved site (InterPro:IPR019801), Glycoside hydrolase, family 35 (InterPro:IPR001944), D-galactoside/L-rhamnose binding SUEL lectin (InterPro:IPR000922), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Galactose-binding domain-like (InterPro:IPR008979); BEST Arabidopsis thaliana protein match is: beta galactosidase 1 (TAIR:AT3G13750.1); Has 2405 Blast hits to 2192 proteins in 479 species: Archae - 15; Bacteria - 1021; Metazoa - 436; Fungi - 211; Plants - 628; Viruses - 0; Other Eukaryotes - 94 (source: NCBI BLink).
Link: InterPro Scan - TAIR