F03024

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F03024

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F03024

Determination of introduced cDNA(s)

  • RAFL04-17-P20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-P20 - Resource: pda00613

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-P20 - full reading 
    CCTGAAAACA TGAACACTCT TCCCGTCGTC TCTCTCACAG CTTCTTCTTC 
    CTTCAAATTC TTCCATTTTC CTTCTCTGCA CCGTTCTCTC TCTCATTCCC 
    CAAACTTCTC CTTCACCAAA TCCCTTATCC TCAATCCAAA CCACCTCTCC 
    TTCAAATCCA CCTTAAATTC TCTTTCACCG TCTCAGTCTC AGCTCTACGA 
    GGAAGAAGAC GACGAAGAAG AAGAAGAAGA AGACGAAGAT GATGATGACG 
    AAGCTGCTGA CGAATACGAC AATATCTCCG ATGAAATTCG GAATAGCGAT 
    GACGACGACG ACGATGAAGA AACCGAATTC TCCGTGGATT TGCCGACGGA 
    ATCAGCTCGG GAACGAGTGG AGTTCCGGTG GCAGAGAGTG GAGAAGCTTC 
    GTAGTCTTGT TAGAGATTTT GGTGTTGAGA TGATTGATAT CGATGAACTC 
    ATTTCAATCT ACGATTTTCG AATCGACAAG TTTCAGAGAT TGGCTATTGA 
    AGCTTTTCTT AGAGGTTCTT CTGTTGTGGT TTCTGCTCCG ACTAGTAGTG 
    GCAAGACTTT GATTGCTGAA GCTGCTGCTG TTTCCACGGT GGCTAAAGGT 
    CGCCGGTTGT TCTACACTAC ACCTCTTAAA GCACTCTCTA ATCAGAAGTT 
    TCGTGAGTTT AGGGAGACAT TTGGGGATGA TAATGTTGGT TTACTCACAG 
    GTGACTCTGA TATTAACAAA GATGCTCAGA TTGTGATTAT GACTACAGAG 
    ATTCTCCGTA ACATGTTGTA TCAAAGTGTT GGAATGGCTT CTTCAGGAAC 
    TGGACTTTTT CATGTTGATG CCATTGTTTT GGATGAGGTT CACTATTTAA 
    GTGATATATC TAGGGGAACT GTGTGGGAAG AGATAGTCAT TTATTGTCCA 
    AAGGAGGTTC AACTTATATG CCTATCAGCT ACAGTTGCAA ATCCGGATGA 
    ATTAGCTGGT TGGATTGGTG AGATACATGG TAAAACTGAG TTGGTAACAT 
    CGACAAGACG CCCAGTTCCA TTAACTTGGT ACTTTTCAAC AAAACATTCT 
    TTATTACCTC TGCTTGATGA GAAAGGGATA AACGTGAATA GGAAGCTTTC 
    TCTCAATTAT TTGCAACTGT CTGCCTCAGA AGCTAGATTC CGTGATGATG 
    ATGACGGGTA TAGGAAAAGG AGGTCAAAGA AACGAGGAGG TGATACCAGC 
    TACAACAACC TTGTGAACGT AACTGATTAT CCTCTTTCAA AGAATGAGAT 
    AAACAAGATT CGTCGATCAC AGGTACCTCA AATTAGCGAC ACATTATGGC 
    ATCTCCAGGG GAAAAATATG TTACCAGCGA TATGGTTCAT TTTTAACCGT 
    AGAGGATGTG ATGCAGCTGT ACAGTATGTT GAAAATTTTC AGCTTTTAGA 
    TGATTGCGAG AAGAGTGAGG TTGAGTTGGC CTTGAAGAAG TTTCGCGTTC 
    TTTATCCAGA TGCAGTGAGA GAGAGTGCAG AGAAAGGACT GCTTAGGGGA 
    ATTGCTGCTC ATCATGCAGG TTGTCTACCT CTTTGGAAAT CTTTCATTGA 
    GGAACTTTTT CAGCGAGGAC TTGTTAAGGT TGTCTTTGCT ACTGAGACAC 
    TTGCTGCTGG AATAAACATG CCGGCTAGAA CGGCTGTTAT CTCGTCTCTT 
    TCAAAAAAGG CTGGTAATGA ACGAATAGAG TTGGGTCCTA ACGAATTGTA 
    CCAGATGGCT GGGCGTGCTG GACGCAGAGG CATCGATGAA AAGGGCTACA 
    CTGTGCTGGT TCAGACTGCT TTTGAAGGCG CTGAAGAGTG CTGCAAACTT 
    GTCTTTGCTG GAGTTAAACC TCTCGTGTCA CAATTTACTG CTTCATATGG 
    CATGGTACTG AATCTGGTAG CTGGTTCAAA AGTTACTCGT AAATCAAGTG 
    GAACTGAGGC CGGGAAAGTC TTACAAGCAG GGAGAAGTCT GGAAGAAGCC 
    AAGAAATTAG TTGAGAAGAG TTTTGGAAAC TATGTGAGCA GCAATGTGAC 
    GGTTGCTGCG AAGCAGGAAC TTGCTGAAAT CGACAATAAG ATCGAGATTC 
    TGTCATCTGA AATAAGTGAT GAAGCAATAG ATAAGAAGAG TAGGAAACTT 
    CTTTCAGCGA GGGATTACAA GGAGATCACT GTACTTAAAG AAGAGCTACG 
    GGAAGAGAAA CGCAAGCGTG CTGAACAACG AAGAAGAATG GAACTCGAAA 
    GGTTCTTAGC TCTAAAACCG CTCTTAAAAG GGATGGAAGA GGGGAACTTA 
    CCATTTATTT GTTTGGAGTT CAAGGATTCT GAAGGGAGGG AGCAGTCAGT 
    CCCTGCTGTT TACTTGGGGC ATATTGATTC ATTTCAGGGT TCCAAACTTC 
    AGAAGATGAT GTCTTTGGAT GAGTCTTTTG CGTTAAACTT AATCGAGGAT 
    GAGCTGGCGG CTGATGAACC TGGAAAGCCT AATGTTAAAC CGTCTTACTA 
    TGTGGCCCTT GGCTCTGATA ACTCATGGTA TCTCTTTACT GAGAAGTGGG 
    TTAGGACTGT CTACAGAACT GGTTTCCCTA ACATTGCTCT AGCGCTTGGA 
    GATGCTTTGC CTCGAGAAAT CATGAAGAAT CTCCTGGATA AAGCAGATAT 
    GCAGTGGGAT AAACTAGCTG AATCTGAACT TGGAAGTTTG TGGAGATTGG 
    AAGGATCTCT AGAGACATGG TCCTGGAGTT TAAACGTACC TGTCCTAAGC 
    AGCCTTTCTG ACGAAGATGA GGTGTTACAT ATGTCCGAAG AGTATGATAA 
    CGCGGCG

    Gene models with high sequence identity

    1. AT1G70070.1 [+] show detail - DEAD/DEAH box helicase, putative
      E-value: 0; Score: 99.93

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart DEAD-like helicase, N-terminal 3.5e-32
    HMMSmart DNA/RNA helicase, C-terminal 1.2e-12
    Gene3D no description 8.4e-18
    HMMPfam DNA/RNA helicase, DEAD/DEAH box type, N-terminal 1.5e-22
    HMMPfam DNA/RNA helicase, C-terminal 2.4e-05
    superfamily P-loop containing nucleoside triphosphate hyd 4.6e-45
    superfamily P-loop containing nucleoside triphosphate hyd 6e-28
    Coil coiled-coil 0
    Coil coiled-coil 0
    ProfileScan Helicase, superfamily 1/2, ATP-binding domain 24.888
    ProfileScan DNA/RNA helicase, C-terminal 8.891
    HMMPanther HELICASE SKI2W 1.2e-211
    HMMPanther HELICASE SKI2W 1.2e-211

    See the detailed result >

    AT1G70070.1

    Model type
    Protein coding
    Short Description
    DEAD/DEAH box helicase, putative
    Curator Summary
    Allelic to ISE2(increased size exclusion limit of plasmodesmata 2). Mutants maintain dilated plasmodesmata at the embryonic torpedo stage.
    Computational Description
    EMBRYO DEFECTIVE 25 (EMB25); FUNCTIONS IN: RNA helicase activity, ATP-dependent helicase activity; INVOLVED IN: posttranscriptional gene silencing, plasmodesmata-mediated intercellular transport, embryo development ending in seed dormancy; LOCATED IN: stress granule, chloroplast stroma, chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), DSH, C-terminal (InterPro:IPR012961), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: RNA helicase, ATP-dependent, SK12/DOB1 protein (TAIR:AT3G46960.1); Has 21396 Blast hits to 12786 proteins in 1473 species: Archae - 1027; Bacteria - 4600; Metazoa - 5457; Fungi - 2724; Plants - 1158; Viruses - 307; Other Eukaryotes - 6123 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR