F08405

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Seed::deep brown

Original: F08405

Determination of introduced cDNA(s)

RAFL02-05-J03 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL02-05-J03 - Resource: pda00237

full reading [+] show sequence

ACCGGACCAA CAGACGGAAT CGGTAAAGCT TTCGCCTTTC AGTTAGCCCA 
GAAAGGTCTT AACCTTATAC TCGTTGCTCG TAACCCAGAC AAGCTCAAAG 
ATGTCTCTGA TTCCATCAGA TCTAAGTATA GTCAAACTCA GATCTTGACC 
GTTGTGATGG ATTTCTCTGG AGATATTGAT GAAGGTGTGA AACGGACTAA 
GGAGAGTATT GAAGGATTAG ATGTTGGGAT TTTGATTAAT AATGCTGGCA 
TGTCTTATCC TTATGCTAAG TATTTTCATG AGGTTGATGA AGAGTTGATC 
AATAACTTGA TTAAGATCAA TGTTGAAGGA ACTACTAAAG TTACTCAAGC 
TGTGTTGCCT AATATGCTTA AGAGTAAGAA AGGTGCTATT ATTAATATGG 
GTTCTGGTGC TGCTGCTCTT ATTCCTTCTT ATCCTTTTTA CTCTGTTTAT 
GCTGGTGCTA AAACGTACGT GGATCAGTTC ACAAAGTGTC TACATGTTGA 
GTATAAGAAG AGTGGGATTG ATGTTCAATG CCAGGTTCCC TTGTATGTTG 
CAACAAAGAT GACAAAAATA AGAAGAGCAT CCTTCTTAGT TGCATCACCA 
GAGGGTTACG CAAAGGCAGC ACTGCGTTTT GTAGGCTATG AAGCACAATG 
CACACCGTAC TGGCCTCACG CTCTCATGGG TGCAGTTGTC TCTGCATTGC 
CCGAAAGCGT TTTTGAATCA TTTAACATCA AGAGATGCCT CCAGATCCGG 
AAGAAGGGTC TCCAAAAAGA CTCCATGAAG AAAGAATGAA TCTTCCAGGT 
TTAAGTTACT ACCAAGAATT TCCTTCTTCT GAAGTTGTTG GTTTCTTGAA 
AAGCTTCTGT TCTGAATCTT TTGTAAGACT TGTACTCTTT AGTTTTCTAA 
GTTTTTTATA TTTTAAGATT AAAAACCCTA ACGTACACGA ATAATTTACT 
TCCTCTGTTT CTATTTATTA ATTTAAGCAG TGTTGTATTG

Gene models with high sequence identity

AT1G67730.1 [+] show detail - beta-ketoacyl reductase 1
E-value: 0; Score: 99.80

InterPro Scan Digest

Program	Description	E-value
FPrintScan	Glucose/ribitol dehydrogenase	2.1e-10
FPrintScan	Glucose/ribitol dehydrogenase	2.1e-10
FPrintScan	Glucose/ribitol dehydrogenase	2.1e-10
FPrintScan	Glucose/ribitol dehydrogenase	2.1e-10
FPrintScan	Short-chain dehydrogenase/reductase SDR	1.2e-05
FPrintScan	Short-chain dehydrogenase/reductase SDR	1.2e-05
FPrintScan	Short-chain dehydrogenase/reductase SDR	1.2e-05
HMMPfam	Short-chain dehydrogenase/reductase SDR	3.1e-18
Gene3D	NAD(P)-binding domain	1.4e-42
HMMPanther	17-BETA HYDROXYSTEROID DEHYDROGENASE	3.3e-86
HMMPanther	Short-chain dehydrogenase/reductase SDR	3.3e-86
superfamily	NAD(P)-binding domain	1.4e-45
PatternScan	Short-chain dehydrogenase/reductase, conserved site	0

See the detailed result >

AT1G67730.1

Model type: Protein coding
Short Description: beta-ketoacyl reductase 1
Curator Summary: Encodes one of the two Arabidopsis homologues to YBR159w encoding a S. cerevisiae beta-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA (very long chain fatty acids, >18 carbon) elongation: KCR1 (At1g67730), KCR2 (At1g24470). Complementation of the yeast ybr159Delta mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene.
Computational Description: beta-ketoacyl reductase 1 (KCR1); FUNCTIONS IN: oxidoreductase activity, ketoreductase activity, acetoacetyl-CoA reductase activity; INVOLVED IN: very long-chain fatty acid biosynthetic process, embryo development, cuticle development; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: beta-ketoacyl reductase 2 (TAIR:AT1G24470.1); Has 86831 Blast hits to 86679 proteins in 3373 species: Archae - 739; Bacteria - 59178; Metazoa - 5127; Fungi - 3946; Plants - 2216; Viruses - 0; Other Eukaryotes - 15625 (source: NCBI BLink).
Link: InterPro Scan - TAIR