F09709

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F09709

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09709

Determination of introduced cDNA(s)

  • RAFL06-14-H08 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-14-H08 - Resource: pda03194

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-14-H08 - full reading 
    GAGGCCGCGA GGACACTTAG AGACAAAGAG CAGCTCGTCA GCTCACCCGT 
    CTGAAGTATC TCGCCAGCCG GCCCGTCTCA AGCAGCTCGT CAGATCACCC 
    GTCTGAAGCA TCTCGTCAGC CGGTCCGTCT CAAGTAGCTC ATCAGCTCGC 
    CCGTCTCAAG CAGCTCGTCA GCTCACCCGT CCGAAGTATC TCGCCAGCCG 
    GCCCGTTAAA TTTTCCCGAT GGAGATTCTT CCGATTCCGG CGGAGAGTTT 
    CAAGGTAGGA TTCATCGGAG CTGGTAAAAT GGCGGAGAGT ATAGCTAGAG 
    GTGTGGTTGC TTCCGGTGTG CTTCCTCCTA ATCGTATATG CACCGCCGTT 
    CACTCAAATC TCAATCGCCG TGATGTCTTC GAATCCTTTG GCGTCAATGT 
    CTTCTCCACT AGCGAAGAAG TTGTTAAAGA AAGCGATGTT GTCATATTCT 
    CTGTGAAACC TCAAGTTGTT AAGAAGGCTG TCACAGAATT AAAGTCGAAG 
    CTTTCAAAGA ATAAGATTCT GGTTTCTGTT GCAGCTGGAA TCAAGTTGAA 
    TGATTTACAG GAATGGTCTG GTCAAGATCG ATTCATAAGG GTGATGCCTA 
    ATACACCTGC CGCTGTTGGT GAGGCAGCTT CAGTTATGAG CCTTGGCACA 
    GGAGCAACGG AAGAGGATGG AGCAATTGTT GCTATGTTGT TTGGCGCGGT 
    GGGGAAGATA TTGAAAGCTG ATGAGAAAAT GTTTGATGCT GTCACTGGTC 
    TCAGTGGAAG TGGACCAGCA TACATATTTT TAGCAATTGA AGCTTTAGCC 
    GATGGAGGAG TAGCTGCTGG TTTACCCCGA GAGCTTGCAT TGAGTTTAGC 
    TTCACAGACC GTTCTTGGAG CTGCAACGAT GGTTAGCAAA ACTGGGAAGC 
    ATCCAGGTGT GTTGAAAGAT GATGTTACCT CACCTGGCGG CACTACAATA 
    GCCGGAGTTC ATGAACTAGA GAAAGGCTCT TTCCGGGCAA CACTTATGAA 
    TGCAGTTGTT GCTGCAGCTA AACGAAGCCG CGAGCTCTCA CAGAGCTAAA 
    TGATACATAT GTAGTTGCTG CTATTGTTTC ACCTCACAGA TTAATCAAAA 
    TAAGGGTTAT GGGCCTTATG GCATTGCTTG TTTTTAGGCG AGAGTTTTAT 
    CCCACTTGTC TTCGATGGTA GAGGTGAAGA TTATTTATCT AGACTATGAT 
    GTATTAGTTC AGACAGAACT CAGATACTTT TCTATAATTC TTAATCTAAT 
    AAAAATCACT TTCAGT

    Gene models with high sequence identity

    1. AT5G14800.1 [+] show detail - pyrroline-5- carboxylate (P5C) reductase
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPIR Delta 1-pyrroline-5-carboxylate reductase 1.8e-127
    Gene3D NAD(P)-binding domain 3.1e-05
    HMMPanther Delta 1-pyrroline-5-carboxylate reductase 3.6e-111
    HMMTigr Delta 1-pyrroline-5-carboxylate reductase 2.2e-97
    HMMPfam NADP oxidoreductase, coenzyme F420-dependent 1.9e-22
    superfamily NAD(P)-binding domain 4.7e-24
    PatternScan Delta 1-pyrroline-5-carboxylate reductase 0

    See the detailed result >

    AT5G14800.1

    Model type
    Protein coding
    Short Description
    pyrroline-5- carboxylate (P5C) reductase
    Curator Summary
    Delta 1-pyrroline-5-carboxylate reductase, catalyzes the final step in proline biosynthesis from glutamate and ornithine.In situ hybridization indicated that under normal growth conditions, the highest concentration of P5CR transcripts occurs in the cortical parenchyma, phloem, vascular cambium and pith parenchyma in the vicinity of the protoxylem. Single gene in Arabidopsis.
    Computational Description
    pyrroline-5- carboxylate (P5C) reductase (P5CR); FUNCTIONS IN: pyrroline-5-carboxylate reductase activity; INVOLVED IN: response to salt stress, response to heat, proline biosynthetic process; LOCATED IN: cell wall, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: NADP oxidoreductase, coenzyme F420-dependent (InterPro:IPR004455), NAD(P)-binding domain (InterPro:IPR016040), Delta 1-pyrroline-5-carboxylate reductase (InterPro:IPR000304); Has 7885 Blast hits to 7882 proteins in 2434 species: Archae - 115; Bacteria - 5234; Metazoa - 373; Fungi - 241; Plants - 70; Viruses - 0; Other Eukaryotes - 1852 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR