F12934

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12934

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12934

Determination of introduced cDNA(s)

  • RAFL09-10-I20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-10-I20 - Resource: pda17635

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-10-I20 - full reading 
    CCCCCGACTG CTCCCAAGTC CTCTCACTTC TTCTTCTTCC TCCGACACCG 
    TTGCTTAACG GTTTTCCATT TTTGTCCCTT CAAGATGATC TCATGGCACG 
    ACCTCTACAC GGTCCTCACC GCCGTGATTC CTCTCTACGT GGCCATGATC 
    CTCGCTTACG GCTCTGTCCG GTGGTGGAAA ATCTTCTCAC CCGACCAATG 
    CTCCGGAATC AACCGTTTTG TCGCCATCTT CGCCGTCCCT CTCCTCTCTT 
    TTCACTTCAT CTCCACCAAC AATCCTTACG CAATGAATCT CCGGTTCATC 
    GCCGCCGACA CTCTCCAAAA AATCATCATG TTGTCACTTT TAGTCCTATG 
    GGCTAATTTC ACTCGCTCCG GTAGCCTCGA GTGGAGCATC ACAATCTTTT 
    CCCTCTCCAC ACTTCCCAAC ACTCTTGTTA TGGGGATTCC TCTCTTGATC 
    GCCATGTATG GCGAATACTC TGGTTCCCTC ATGGTCCAAA TCGTCGTCCT 
    CCAGTGTATC ATCTGGTACA CGCTTCTCCT TTTTCTCTTC GAGTTTCGTG 
    GCGCCAAGAT GCTCATCATG GAGCAGTTCC CTGAGACGGC TGCTTCCATT 
    GTTTCTTTCA AAGTCGAATC CGACGTCGTT TCGCTCGACG GCCATGATTT 
    TCTTGAGACC GATGCAGAGA TAGGTGACGA CGGGAAGCTT CACGTCACCG 
    TGAGAAAATC CAACGCTTCA CGTCGTTCGT TCTGCGGCCC GAACATGACT 
    CCACGGCCGT CAAATCTCAC CGGAGCTGAG ATTTATAGTC TCAGCACCAC 
    TCCTAGAGGC TCTAATTTCA ACCACTCTGA TTTTTACAAC ATGATGGGTT 
    TCCCCGGTGG TCGTCTCTCC AATTTCGGTC CGGCGGATAT GTACTCCGTT 
    CAATCATCTA GAGGTCCAAC TCCTCGACCT TCAAACTTCG AGGAGAATTG 
    CGCCATGGCA TCCTCCCCGA GATTCGGGTA TTACCCTGGA GGAGGAGCCG 
    GGTCTTATCC GGCTCCGAAT CCAGAGTTCT CTTCAACCAC CACATCTACC 
    GCCAATAAAA GCGTCAATAA AAACCCGAAA GACGTTAATA CGAATCAGCA 
    GACGACTCTT CCAACGGGCG GCAAGTCAAA CAGCCATGAC GCCAAGGAGC 
    TTCACATGTT CGTCTGGAGC TCAAACGGGT CACCCGTTTC AGACCGGGCG 
    GGTCTTAACG TTTTCGGCGG AGCACCTGAC AACGATCAAG GCGGAAGATC 
    TGACCAAGGT GCTAAAGAGA TCCGTATGTT AGTCCCAGAT CAATCTCACA 
    ACGGCGAGAC CAAAGCTGTA GCTCATCCAG CAAGTGGAGA TTTCGGAGGA 
    GAACAACAAT TTAGTTTCGC CGGAAAAGAA GAAGAAGCAG AGAGACCAAA 
    AGACGCCGAG AATGGTCTAA ACAAACTTGC TCCAAATTCC ACGGCGGCGC 
    TACAATCCAA GACAGGTCTA GGAGGAGCCG AAGCAAGTCA ACGAAAAAAT 
    ATGCCTCCGG CGAGTGTGAT GACAAGGCTG ATACTGATAA TGGTTTGGAG 
    GAAACTCATC AGAAACCCAA ACACTTACTC TAGTCTCATT GGACTTATTT 
    GGGCTCTCGT CGCTTTCCGG TGGCACGTGG CAATGCCCAA AATCATTCAA 
    CAATCTATCT CCATTCTATC TGATGCTGGT CTTGGAATGG CAATGTTTAG 
    TTTGGGGTTG TTCATGGCGT TGCAACCCAA ATTAATCGCT TGTGGGAATT 
    CAGTGGCAAC GTTTGCCATG GCGGTTAGGT TCCTTACGGG TCCGGCCGTG 
    ATGGCGGTTG CTGCTATAGC CATCGGATTA CGTGGTGATT TACTGCGTGT 
    CGCTATAGTT CAGGCCGCAT TACCTCAAGG AATTGTGCCC TTTGTGTTTG 
    CGAAGGAGTA CAATGTTCAT CCTGCTATTT TAAGTACAGG GGTAATATTT 
    GGAATGCTTA TAGCGCTTCC GATCACGCTG GTTTACTACA TTCTACTCGG 
    GTTATAAGCT TTCGGTTATG AAAGCCCCGA GAAAGAGTCA ATTGTAGAGG 
    GAGAAGGAAG AAAGGGAAAC TAGAGAGTGT CTCATGGAAC AAACTAAAAA 
    AAGCTTAGAG TATGTAAGTA CAACTAGGGA GTAGCATGAT TTAAGGAATG 
    CTGATGCCAA CATTACAAGC CAAGAAACCC TTTCTTCTCC CATATTTTTT 
    ATATATAGAA TATTGGGAGA TTATTTGAGT GAGACAATGG GACGCAGATA 
    AGGAGGAGCA AAACATGGGG CAACGAGACA AAGAAAAGAT AAATAAAAGA 
    TTGGAAGATG AAGACAACTT ATTATTTTTG TAACTTTTAT TTTTCTTACA 
    CAGTCTTTTT TTTTTTTTTT TGTGTTCTTT GATTAGGTTC GGGTAACTCT 
    TAGTTGATTA TTGTGTAGAT TTTTTTTAGC GAAGAAAAGT GTGGTTTTGA 
    TTTTGAACAA AAAAA

    Gene models with high sequence identity

    1. AT1G70940.1 [+] show detail - Auxin efflux carrier family protein
      E-value: 0; Score: 99.96

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Auxin efflux carrier, subgroup 0
    Coil coiled-coil 0
    HMMPfam Auxin efflux carrier 6.7e-187

    See the detailed result >

    AT1G70940.1

    Model type
    Protein coding
    Short Description
    Auxin efflux carrier family protein
    Curator Summary
    A regulator of auxin efflux and involved in differential growth. PIN3 is expressed in gravity-sensing tissues, with PIN3 protein accumulating predominantly at the lateral cell surface. PIN3 localizes to the plasma membrane and to vesicles. In roots, PIN3 is expressed without pronounced polarity in tiers two and three of the columella cells, at the basal side of vascular cells, and to the lateral side of pericycle cells of the elongation zone. PIN3 overexpression inhibits root cell growth. Protein phosphorylation plays a role in PIN3 trafficking to the plasma membrane.
    Computational Description
    PIN-FORMED 3 (PIN3); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: in 8 processes; LOCATED IN: lateral plasma membrane, plasma membrane, vesicle membrane, cell surface; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G23080.1); Has 1983 Blast hits to 1757 proteins in 550 species: Archae - 37; Bacteria - 1198; Metazoa - 14; Fungi - 0; Plants - 487; Viruses - 0; Other Eukaryotes - 247 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR