F13333

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Seed::deep red
Seed::light blue

Original: F13333

Determination of introduced cDNA(s)

RAFL09-90-L22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-90-L22 - Resource: pda17902

full reading [+] show sequence

GGACTATACA CAGAATACAA AAGAGATATT ATATGTAATT ATCAATAGTA 
TATGACAAAT TTATAGCCTC ACCTTTCTTC TTCTTCTTCT TCTTCTTCTG 
TGTGTTTTTG AATTTGCTTT CTTCAAGAGG TTTCTTCTCC ACACCGCTTC 
GGTGTATGAT CTGATTGTGA TCTCTCTCGA TATCTGGGAT CTTCGGGGTA 
TGGAGGCTGT AGATTATTAT TATTATTACG TGTGGTGCTG ATGCAATGAG 
TGTTGATGCC TGTTATGGAA AGAGTAGCTG AAGAAACTGT GGCGACTAAC 
AACATACAAT CAAAAGCACG AGTGGATGAT GTTCCTTGTA ATAAGCTTGA 
TGCTCGTCAT AACGATATGG TGATTCAATC AGAAACTGCA AACAGTGATT 
GTCCTGGAAG CTCAGCTCAC AGGAATGTTG ATTTGACTAA GCCGCCGCCT 
CCTGAGGAAG CCGCAGGAGC TAAGCTTTCT GTTGAAGAGT TGACATTGGG 
GAACTACAGG ATTGTTCAAG GGAGTAATAA TACTAATGTG GATAGTCCTA 
GGGCTGGCAA GTTTGAGCAT CTTTATCGTT TGGCTAGAGG GTCTGCTTTT 
AGGGCAGGAG ATGGGGATCT TGATTCTCAA CCTCGTGATA TGGATCAAAT 
GTTGTCCAGA ATCAGACAAC AGCTTGCTGG AGCTCCTTCT GAGAGGCAAA 
ACTTGAAACC TTTTATGTCT AGGAGGAGCG ATCAGAATCT TGAAGCTTTT 
TCTGAACGTC TAAGAGCAGC TGGTGAGAAT AGTATTATGA ATGCTCCTGC 
ATTGATCAGT GAAGGTGTCC AAATGAAAAC GCCGGTGAGT TCTTCGAATT 
TTTCTCAGTT GTTACTTAAA AGAGCTATGA AAGGGAAAGG GGTAGTAGGA 
AAGAACCAGG AAACTCCTCC TGAATTTGTC TCTGATCAAG ATTTAGGAAG 
CAAGGAAAAA AAGTTGGATA TTAGCAAGTC GCCTACTCCT CACGATGTGT 
TACCTCTAAA ATCAAGTCCC AAAGGAAATG GTATGGTATC ACATGGTGAT 
GGGAATCATA GTAAATCTTC TATTGGAATC AGTTTGAGGG AGTTTCTTAG 
ATCAAGTTAT GCTAAAAGAG AAAAAAGACA TGGCCTTTGT CTATTTAGGC 
AGCTGGTGGA ACTGGTTGAC TCAGCCCATT CAAAAAGATT ATTCTTGCTA 
GACTTGAGAC CATCCCTTTT CACTTTAGTT CCATCAAAGA AACTTAGATA 
TATTGGTAAT TTTGGAAAGA ACGATCTAGA ATCAGATGTT GACGAAGATT 
TGAATAGGAG GAGGCCTGTT GTTGAGGAAT CATCTAGTGG AGGTAGGGAT 
TCGAAGAAAA GAAAAATGGA CTTGCATTTG AATTCTCCAG GGAATCAACT 
TCAGGCCACT TCAACCGGGC GACCTTTCAA AAGGAAAAGC CCTGTGATTG 
ATTTAAACAT GGTTGATGCA CGTAATCCAG ACAGTTGCGA GCTTCAACAG 
CAAGACTACA TAAAAAATTT AAGCGTGTCT TCAGTGTCGA GAAAACAGTC 
TATGTCCACA TGGCTAGAAG AGCAATGGTA TACTTGTCCA GAGGAGATAA 
ATGGAGAAGA TATTGGAGAA AAATCAAATA TTTATGCCCT TGGTGTTCTT 
CTGTTTGAGT TGCTATGCCA TTGCGAGTCA GGCGAGATGC ATGCTGCAAT 
GATGGCAGAT TTACGCCATC GGATTCTCCC ACCAACATTT CTCTCGAAAT 
ACCCGAAGGA AGCTGGATTT TGTCTTTGGC TTCTACATCC TGAACCCTCC 
TCCCGACCAT CAGCTAGAGA TATACTAAAG TCAGAGTTGA TATGCGAGGA 
TGATTCAGTT AAATCGACTG CTGCTGCTGA GGAGATATCT GAGCTGTTAC 
TTCATTTTTT GTCTTCATTA GAAGTGCAGA AGAAGAAAAA AGCATCTAAA 
CTTTTGCAAG ATATCCAGAC CTTAGAGGAT GATATTAAGG AGGCCGAGCG 
AAGATATTCT TCAAATGTAT CTCTGGTGAG ATCTCATGGA GCTATTGAAA 
AAAGGGTGCA ATCATCTCCC CTAGACGAGC ACTGTACAAC TTCCAGTGCC 
TTGTTTGTAC CAACTGCTAA TACAGACAGG CTGATGAGTA ATATCCGTCA 
ACTTGAAGAT GCATATTTTT TCATGCGATC ACAAATCAAC TTATCGAGTT 
CTGCTGCTAC TGCCCGTTCT GACAAAACAC TAAAGGACAG GGACAGATGC 
TCTGAAAACC AAAATGAAAA TCAGGATATG AGTACCAAAG GAAAATCTTC 
AGATCAACTC GAAGTGTTTT TCGAGGGGTT GTGCAAATTT GCTCGGTATA 
GCAAGTTCGA AACCTGTGGG ACAATAAGAA GTGGGGACCT TTTAAACTCT 
GCAAGTGTGG TCTGCTCGTT GAGTTTTGAC CCTGATGAAG AACACATAGC 
AGCAGCTGGG ATATCAAAGA AGATCAAGAT TTTTGACTTC AATGCATTTA 
TGAATGAATC TGTCGGTGTT CATTATCCAC TAGTAGAGAT GGTCAACAAA 
TCTAAATTAA GCTGTGTTTG CTGGAATAGC TACATCAAAA ACTACTTGGC 
CTCGACTGAC TACGATGGTG TAGTTCAGAT ATGGGATGCC GGCACTGGAC 
AAGGATTTTC GCAGTACACA GAACACCAGA AGAGAGCCTG GTCAGTTGAT 
TTTTCTCCAT CTGACCCGAC AAAATTTGTT AGTGGAAGTG ATGACTGCTC 
GGTGAAGCTT TGGAGCATCA ATGAGAAACG TTCATTAGGC ACAATCTGGA 
GTCCAGCAAA TGTGTGTTGC GTGCAATTCT CTTCTTATTC CAATCACTTA 
TTGGCATTTG GGTCAGCTGA TTACAAGGTC TATTGCTATG ACCTTCGGTA 
TGTCAAAACA CCTTGGTGCA CATTGGCTGG TCATGAGAAA GCGGTGAGTT 
ATGTTAAATT TATGGATTCA GAAACCATTG TTTCTGCTTC CACTGATAAC 
TCTCTCAAGC TTTGGAATCT TAACAAGACA AATTCCTCTG GTTTATCTCC 
TGGTGCTTGT TCATTGACAT ATAAAGGACA TACAAATCAG AAGAATTTCG 
TTGGATTGTC GGTTTTGGAC GGATACATAG CTTGTGGTTC AGAGACCAAT 
GAGGTATATA GTTACTATAA ATCTTTACCG ATGCCAATGA CTTCATACAA 
GTTTGGATCC GTCGATCCCA TTTCTGGAAA CGAATACTTT GATGACAATG 
GACAGTTTGT GTCGAGCGTC TGTTGGAGGA AAAAATCGAA TATGCTTGTA 
GCTGCAAACT CTACTGGAAA CATGAAGCTA CTAAAACTTG TTTGACAACT 
TGGCCTTTTC GCTACTACTG CGGGCATTGG ACTTTTTGAG TCTGAAACTA 
GGACAAAGCA GGTCTGACTT TGAAGCAAAC GTTTTTGTCC TGGAAACTAT 
GATCCCATAT GACCAGAAGA GAAAAAAAGT TCGAAATCTT GGAGAAGTTG 
AAGAAGCAAA ACTGTCTGTG ATAATATATT ATTTGATTCT TCTTCCTGTA 
ATTCTATATA TATGAGATAT AATTTGAGAT AAACTTCTCA GATTCTTCAG 
AAATGATTCA TATTTGGTCG GCATAACGTG TAATGATGAG AGGTATATAA 
CTTGTACAAG TGTGTTATAT CGTTAGCTCG GTTATGTAAT TGTTGTTGGA 
GATTCTCTAT AAGAGAGAAA ATATATATAT CATCCATTCA TTGCAAAAAA 
AAAA

Gene models with high sequence identity

AT2G46340.1 [+] show detail - SPA (suppressor of phyA-105) protein family
E-value: 0; Score: 99.95

InterPro Scan Digest

Program	Description	E-value
PatternScan	WD40 repeat, conserved site	0
PatternScan	WD40 repeat, conserved site	0
HMMPfam	WD40 repeat, subgroup	0.043
HMMPfam	WD40 repeat, subgroup	0.0091
HMMPfam	WD40 repeat, subgroup	1.6e-07
HMMPfam	WD40 repeat, subgroup	7.4e-06
HMMPfam	Serine/threonine-protein kinase-like domain	1.5e-07
Gene3D	no description	7.7e-11
Gene3D	WD40/YVTN repeat-like-containing domain	1.5e-51
HMMSmart	WD40 repeat	0.12
HMMSmart	WD40 repeat	0.18
HMMSmart	WD40 repeat	2.4e-07
HMMSmart	WD40 repeat	0.52
HMMSmart	WD40 repeat	5.2e-05
HMMSmart	WD40 repeat	96
HMMSmart	WD40 repeat	180
superfamily	WD40 repeat-like-containing domain	2.6e-53
superfamily	Protein kinase-like domain	2.6e-13
Coil	coiled-coil	0
ProfileScan	Protein kinase, catalytic domain	9.263
ProfileScan	WD40 repeat 2	12.079
ProfileScan	WD40 repeat 2	12.413
ProfileScan	WD40-repeat-containing domain	25.338
HMMPanther	UBIQUITIN LIGASE PROTEIN COP1 (CONSTITUTIVE P	2.9e-69
HMMPanther	WD40 REPEAT PROTEIN	2.9e-69
FPrintScan	G-protein beta WD-40 repeat, region	5.9e-05
FPrintScan	G-protein beta WD-40 repeat, region	5.9e-05
FPrintScan	G-protein beta WD-40 repeat, region	5.9e-05

See the detailed result >

AT2G46340.1

Model type: Protein coding
Short Description: SPA (suppressor of phyA-105) protein family
Curator Summary: Encodes a member of the SPA (suppressor of phyA-105) protein family (SPA1-SPA4). SPA proteins contain an N-terminal serine/threonine kinase-like motif followed by a coiled-coil structure and a C-terminal WD-repeat domain. SPA1 is a PHYA signaling intermediate, putative regulator of PHYA signaling pathway. Light responsive repressor of photomorphogenesis. Involved in regulating circadian rhythms and flowering time in plants. Under constant light, the abundance of SPA1 protein exhibited circadian regulation, whereas under constant darkness, SPA1 protein levels remained unchanged. In addition, the spa1-3 mutation slightly shortened circadian period of CCA1, TOC1/PRR1 and SPA1 transcript accumulation under constant light.
Computational Description: SUPPRESSOR OF PHYA-105 1 (SPA1); CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), Protein kinase, catalytic domain (InterPro:IPR000719), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: SPA1-related 2 (TAIR:AT4G11110.1); Has 31438 Blast hits to 19748 proteins in 684 species: Archae - 32; Bacteria - 4770; Metazoa - 12431; Fungi - 6743; Plants - 3442; Viruses - 2; Other Eukaryotes - 4018 (source: NCBI BLink).
Link: InterPro Scan - TAIR