F13638

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13638

Determination of introduced cDNA(s)

RAFL07-11-A11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-11-A11 - Resource: pda04115

full reading [+] show sequence

AAAATTCACT CAACTTTCTC TCACTCCTTT TTTCTCTCTC TCTCTCTATC 
CTCTACAGAG CTCGCAGCAA CTAGCGCAGG TACTCTCTCT TTCTCACTCC 
TTAATCTAGA TACTAAAGCT GATCATTTTC ATATCTCTCA GTAATCCGTT 
TCGTCTGATC GGGATGTTGG AGAAGGTTGA AACTTTCGAC ATGAACAGAG 
TCATTGATGA ATTCGATGAA ATGACTAGAA ACGCTCATCA AGTCCAGAAA 
CAAACTTTGA AAGAGATTCT TCTCAAGAAC CAATCCGCCA TTTACTTACA 
AAACTGTGGT CTCAATGGAA ACGCTACTGA CCCTGAAGAA GCTTTCAAAT 
CTATGGTTCC TTTAGTCACT GATGTTGAAT TAGAGCCTTA CATCAAAAGA 
ATGGTTGATG GTGACACTTC ACCTATTCTC ACTGGTCACC CTGTTCCTGC 
CATTTCCTTA AGCTCTGGAA CTAGTCAAGG CCGTCCAAAG TTTATTCCTT 
TCACTGATGA ATTAATGGAG AACACATTGC AACTGTTTCG CACTGCTTTT 
GCCTTCAGAA ACAGAGATTT TCCCATTGAT GACAATGGCA AAGCTTTGCA 
ATTTATCTTC AGCAGCAAGC AGTACATATC AACGGGAGGT GTCCCGGTTG 
GAACCGCAAC CACAAACGTG TACCGCAACC CTAACTTCAA AGCTGGGATG 
AAATCTATAA CGTCTCCTTC CTGTAGTCCC GACGAAGTTA TCTTTAGTCC 
TGATGTCCAT CAAGCCTTGT ATTGCCATCT CTTATCCGGA ATTCTCTTCA 
GAGATCAAGT TCAATACGTC TTTGCCGTCT TTGCTCACGG TCTAGTTCAC 
GCTTTTAGAA CCTTTGAACA GGTTTGGGAA GAGATTGTTA CCGATATCAA 
AGATGGTGTC TTAAGCAACC GTATTACTGT CCCATCGGTC CGTACTGCAA 
TGTCGAAGCT GCTCACACCT AACCCTGAGC TAGCTGAGAC GATCCGCACC 
AAATGTATGA GTTTGAGCAA CTGGTACGGA TTGATTCCCG CGCTCTTCCC 
CAACGCAAAG TATGTTTACG GGATCATGAC TGGCTCCATG GAGCCGTATG 
TGCCAAAGCT GAGACATTAC GCGGGTGATC TACCTCTTGT GAGCCATGAC 
TACGGTAGCT CGGAAGGATG GATTGCTGCT AATGTTACCC CAAGGTTATC 
TCCAGAGGAA GCTACATTTG CTGTGATTCC GAATCTCGGT TATTTCGAGT 
TTCTCCCTGT ATCTGAAACA GGGGAAGGAG AGGAGAAACC GGTTGGTTTA 
ACTCAAGTCA AGATTGGAGA AGAGTATGAA GTTGTCATCA CAAATTACGC 
AGGGTTGTAT CGATACCGGC TTGGAGATGT GGTAAAGGTC ATTGGTTTCT 
ACAACAACAC TCCCCAACTA AAATTCATAT GCAGGAGAAA CCTGATTCTC 
TCGATCAACA TCGACAAGAA CACCGAAAGA GACCTTCAGC TTTCAGTAGA 
ATCGGCTGCA AAGAGACTCT CAGAAGAAAA GATCGAGGTC ATAGACTTCT 
CTAGCTACAT CGATGTCTCG ACAGATCCAG GACATAACGC TATCTTCTGG 
GAGATTTCAG GAGAAACAAA CGAAGACGTT CTTCAAGATT GCTGCAACTG 
TTTAGACCGA GCATTCATCG ATGCGGGTTA CGTTAGTTCC CGGAAATGCA 
AGACTATTGG AGCCTTGGAG CTGAGAGTTG TCGCAAAAGG AACTTTCCGG 
AAGATTCAAG AACATTTTCT CGGGCTTGGT TCATCGGCTG GACAGTTTAA 
GATGCCAAGA TGTGTGAAGC CAAGTAATGC TAAGGTTCTC CAAATTCTGT 
GTGAGAATGT GGTGAGTAGC TACTTCAGCA CAGCGTTTTG ACTCACTAAA 
ATTGTGTGAA ATCAAAGAAA GTGGTTTTTA GTTGTTTATT TCCGTCGTTG 
TTGTCTTTAT TTGTCAATAT AGTTTTCTTT ACGTTATTTA ATTGAGCATA 
AACTTTTTTT ATCTCAAAAA AAAAAAAAAA A

Gene models with high sequence identity

AT2G46370.1 [+] show detail - Auxin-responsive GH3 family protein
E-value: 0; Score: 99.95

InterPro Scan Digest

Program	Description	E-value
Coil	coiled-coil	0
HMMPfam	GH3 auxin-responsive promoter	1.6e-178

See the detailed result >

AT2G46370.1

Model type: Protein coding
Short Description: Auxin-responsive GH3 family protein
Curator Summary: Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.
Computational Description: JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).
Link: InterPro Scan - TAIR