F14517

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F14517

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F14517

Determination of introduced cDNA(s)

  • RAFL05-12-H19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-12-H19 - Resource: pda12227

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-12-H19 - full reading 
    AGGTTACTTG TTAGATCTCA ACAACCAGGT CGCACACACT AAAATGGCGT 
    TCCGCGTTTT GCTCCTCTTC TCACTCACCG CACTTCTCAT CTTCTCCGCC 
    GTTTCTCCTT CTTTCGCCGC TTCTTCCTCC GACGACGTTG ACGATGAGGA 
    TCTCAGCTTC CTCGAAGATC TCAAAGAAGA CGACGTTCCC GGCGCTGACT 
    CACTCTCTTC CTCAACTGGA TTTGACGAAT TCGAAGGAGG AGAAGAAGAA 
    GATCCCGATA TGTACAACGA CGATGACGAT GAAGAAGGAG ATTTTTCCGA 
    TCTAGGTAAT CCGGATTCCG ATCCGTTACC GACGCCGGAG ATTGATGAGA 
    AAGACGTTGT GGTTATCAAG GAGCGTAACT TCACTGATGT GATTGAGAAT 
    AATCAATACG TTTTGGTTGA GTTTTATGCT CCGTGGTGTG GTCATTGTCA 
    GTCTCTTGCT CCTGAGTATG CTGCTGCTGC CACGGAGCTT AAGGAAGATG 
    GTGTTGTTTT GGCTAAGATT GATGCGACGG AGGAGAATGA GTTAGCTCAG 
    GAGTATCGTG TTCAGGGCTT TCCGACGCTT CTTTTCTTCG TTGATGGTGA 
    GCACAAGCCT TACACTGGAG GAAGGACTAA AGAAACAATT GTGACATGGG 
    TGAAGAAGAA GATTGGTCCT GGTGTGTATA ATCTAACTAC ATTAGATGAT 
    GCTGAGAAAG TGTTGACTTC TGGGAACAAA GTTGTTTTGG GATACTTGAA 
    CTCCTTGGTG GGTGTTGAGC ACGATCAGCT TAATGCTGCT TCCAAAGCTG 
    AAGACGATGT GAACTTTTAT CAAACAGTGA ATCCTGATGT TGCAAAGATG 
    TTTCACCTTG ATCCGGAGTC TAAAAGGCCT GCTCTTGTCC TAGTTAAGAA 
    GGAAGAGGAG AAAATTAGCC ATTTTGATGG AGAATTTGTT AAGTCTGCTC 
    TAGTTAGTTT TGTGTCTGCC AACAAGCTTG CTTTGGTCTC TGTTTTCACC 
    AGAGAGACTG CTCCGGAAAT TTTTGAGAGT GCAATCAAGA AACAGTTGTT 
    GTTGTTTGTA ACCAAAAATG AATCTGAAAA GGTTCTTACG GAATTTCAAG 
    AAGCAGCGAA ATCATTCAAA GGAAAGCTCA TCTTTGTATC TGTGGATCTG 
    GATAATGAGG ATTATGGGAA GCCAGTCGCT GAATACTTTG GTGTGTCTGG 
    AAATGGTCCT AAACTTATTG GCTACACAGG GAATGAAGAC CCTAAAAAAT 
    ACTTTTTCGA TGGCGAGATC CAGTCAGATA AAATTAAGAT ATTTGGGGAG 
    GATTTCCTGA ACGACAAGTT AAAGCCTTTC TATAAGTCAG ACCCCATTCC 
    TGAAAAGAAC GATGAAGATG TGAAAATAGT GGTTGGAGAT AACTTTGATG 
    AAATTGTTCT GGACGATTCT AAAGATGTGC TTCTCGAGGT CTACGCACCA 
    TGGTGTGGCC ATTGCCAAGC CCTTGAGCCA ATGTATAACA AACTTGCCAA 
    GCATTTACGA AGTATTGATT CTCTCGTCAT AACCAAGATG GATGGAACAA 
    CCAATGAACA TCCCAAGGCA AAGGCTGAGG GGTTCCCTAC CATTCTCTTC 
    TTCCCTGCGG GCAACAAGAC TTCAGAGCCG ATTACTGTAG ATACAGACCG 
    CACTGTGGTT GCATTTTACA AGTTCTTAAG GAAACACGCA ACGATCCCAT 
    TCAAACTGGA GAAACCTGCA TCAACCGAAT CACCTAAAAC TGCAGAGTCC 
    ACACCAAAAG TAGAAACTAC CGAGACCAAA GAATCACCCG ATAGCACGAC 
    AAAGAGTAGC CAAAGTGACT CGAAGGACGA ATTGTGATCA GAAAAGGGTA 
    TAATATATAT TACATGTCTC CTTAAGTTGT TATATGTATG ATCAGAATTG 
    TCACATCATG AGTTTGGACT AGATAAAGAT GGGAATTAAA GTAATTTTAG 
    TTTTGAGAT

    Gene models with high sequence identity

    1. AT5G60640.1 [+] show detail - PDI-like 1-4
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Thioredoxin fold 2e-35
    Gene3D Thioredoxin fold 7.4e-24
    Gene3D Thioredoxin fold 1.6e-21
    HMMPfam Thioredoxin domain 5.4e-32
    HMMPfam Thioredoxin domain 1.2e-21
    superfamily Thioredoxin-like fold 6.9e-36
    superfamily Thioredoxin-like fold 1.4e-27
    superfamily Thioredoxin-like fold 2.8e-18
    superfamily Thioredoxin-like fold 2.1e-17
    HMMPanther PROTEIN DISULFIDE ISOMERASE 3.4e-113
    ProfileScan Thioredoxin-like 17.347
    ProfileScan Thioredoxin-like 12.482
    HMMTigr Protein disulphide isomerase 3.8e-267
    HMMTigr Disulphide isomerase 9.8e-48
    HMMTigr Disulphide isomerase 1.1e-30
    FPrintScan Thioredoxin-like subdomain 1.4e-06
    FPrintScan Thioredoxin-like subdomain 1.4e-06
    FPrintScan Thioredoxin-like subdomain 1.4e-06
    PatternScan Thioredoxin, conserved site 0
    PatternScan Thioredoxin, conserved site 0

    See the detailed result >

    AT5G60640.1

    Model type
    Protein coding
    Short Description
    PDI-like 1-4
    Curator Summary
    Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily. Unlike several other PDI family members, transcript levels for this gene are not up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin). However, the level of transcripts for this gene is slightly elevated in atbzip60 mutants.
    Computational Description
    PDI-like 1-4 (PDIL1-4); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: response to oxidative stress; LOCATED IN: mitochondrion, endoplasmic reticulum, plasma membrane, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Disulphide isomerase (InterPro:IPR005788), Thioredoxin domain (InterPro:IPR013766), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Protein disulphide isomerase (InterPro:IPR005792), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 1-3 (TAIR:AT3G54960.1); Has 27268 Blast hits to 18617 proteins in 2977 species: Archae - 300; Bacteria - 12440; Metazoa - 5251; Fungi - 1704; Plants - 2409; Viruses - 19; Other Eukaryotes - 5145 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR