F15123

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F15123

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F15123

Determination of introduced cDNA(s)

  • RAFL05-07-D07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-07-D07 - Resource: pda02466

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-07-D07 - full reading
    GTTCATCAGA TCTACTACAA TCTCTCTTTT TCTTCGGGTA TGGAGGGCCA 
    ACGAGGCAGT AATTCTTCGT TGAGCTCTGG CAATGGTACC GAAGTCGCCA
    CCGACGTTTC TTCTTGCTTC TATGTTCCCA ATCCCTCTGG AACCGATTTC
    GATGCCGAGT CGTCTTCTCT TCCTCCTCTC TCCCCAGCTC CTCAAGTGGC
    ATTGTCAATT CCTGCGGAGC TTGCCGCCGC CATTCCCCTC ATCGATCGCT
    TCCAGGTTGA AGCTTTTCTG CGGCTAATGC AGAAACAAAT CCAGTCTGCT
    GGGAAGCGTG GCTTCTTCTA TTCCAAAAAG TCCTCTGGCT CCAATGTCCG
    CGAGCGCTTC ACATTTGAGG ATATGCTTTG CTTTCAAAAG GATCCAATCC
    CCACATCTCT CCTCAAGATT AACAGCGATC TCGTCAGCCG TGCTACCAAG
    TTGTTTCATC TCATCTTAAA ATATATGGGT GTTGATTCAT CTGATCGATC
    TACGCCTCCC AGTTTAGATG AACGCATTGA CCTCGTTGGA AAGCTCTTCA
    AAAAAACTTT GAAGCGTGTT GAACTCAGGG ACGAACTTTT TGCCCAAATC
    TCCAAACAGA CTAGACATAA TCCTGACAGG CAATACTTGA TCAAAGCTTG
    GGAATTGATG TACTTATGTG CCTCCTCTAT GCCTCCTAGC AAAGATATCG
    GTGGATATCT ATCTGAGTAT ATTCATAATG TCGCACACGA TGCAACTATT
    GAACCGGATG CTCAGGTTCT TGCTGTTAAC ACTTTGAAAG CTTTAAAGCG
    CTCTATCAAA GCTGGTCCTA GGCACACCAC ACCTGGTCGT GAAGAAATTG
    AAGCCCTTTT GACCGGTAGA AAGCTCACAA CCATTGTCTT CTTTCTCGAT
    GAAACTTTTG AAGAAATTTC ATATGACATG GCTACCACAG TGTCTGATGC
    TGTTGAGGAG CTAGCTGGAA CAATTAAACT ATCAGCTTTC TCTAGCTTTA
    GTTTGTTTGA ATGTCGTAAA GTTGTTTCAA GTTCTAAATC ATCTGATCCC
    GGAAATGAGG AATATATAGG ATTGGATGAT AACAAGTATA TTGGAGATCT
    CCTCGCAGAA TTTAAAGCTA TTAAAGATCG AAATAAAGGA GAGATACTAC
    ACTGCAAACT GGTATTTAAA AAAAAATTAT TCCGAGAGTC TGATGAAGCT
    GTAACAGATC TGATGTTTGT GCAACTTTCG TATGTTCAAC TGCAACATGA
    CTATTTGCTA GGAAACTATC CTGTTGGGAG GGACGATGCT GCACAGCTTT
    GTGCCTTGCA AATTCTTGTT GGGATTGGGT TTGTCAATAG TCCAGAGTCA
    TGCATTGATT GGACATCACT TCTTGAGCGG TTTTTGCCAA GACAAATAGC
    AATAACCCGA GCAAAGCGTG AATGGGAATT GGATATCCTT GCTCGCTACC
    GTTCAATGGA GAACGTGACC AAAGATGATG CAAGACAACA ATTTCTACGG
    ATACTGAAGG CACTGCCATA CGGGAATTCA GTTTTTTTTA GCGTACGCAA
    GATAGATGAT CCGATCGGTC TTTTACCTGG GCGAATCATT TTGGGTATCA
    ACAAACGTGG GGTTCACTTT TTTCGACCGG TTCCTAAAGA ATATCTGCAC
    TCTGCTGAAC TACGTGACAT CATGCAATTT GGCAGCAGTA ACACTGCTGT
    CTTTTTCAAA ATGAGAGTCG CTGGTGTTCT TCACATATTT CAGTTCGAGA
    CAAAACAGGG AGAAGAAATT TGTGTTGCTT TGCAAACACA TATAAATGAT
    GACATGTTGC GTCGTTACTC CAAAGCTCGA TCTGCTGCCA ATAGCTTGGT
    TAATGGAGAT ATATCTTGTA GTTCTAAGCC GCAAAATTTT GAAGTGTATG
    AAAAACGTTT GCAAGATTTG TCTAAGGCTT ATGAAGAGTC CCAAAAGAAG
    ATTGAGAAGT TGATGGATGA ACAACAAGAG AAAAATCAGC AAGAAGTTAC
    TCTGCGTGAA GAGTTAGAAG CTATACACAA TGGTTTGGAG CTTGAAAGGA
    GAAAATTGTT GGAGGTTACT TTAGACCGAG ATAAACTTAG GTCATTGTGT
    GACGAGAAGG GAACAACTAT TCAATCCTTG ATGTCTGAAC TGCGAGGAAT
    GGAAGCAAGG TTGGCAAAGT CGGGCAACAC AAAATCAAGT AAAGAGACAA
    AATCAGAATT AGCCGAAATG AATAATCAGA TATTATACAA GATCCAAAAG
    GAGTTAGAAG TTCGAAATAA GGAATTGCAT GTCGCAGTTG ATAATTCAAA
    GAGGTTGTTG AGTGAAAACA AGATATTGGA GCAAAATCTC AATATTGAAA
    AGAAGAAAAA AGAGGAGGTT GAAATTCATC AAAAGAGATA TGAACAAGAA
    AAAAAGGTGT TAAAGCTTCG AGTTTCTGAA CTTGAAAATA AGCTTGAAGT
    ACTTGCTCAA GACTTGGATA GTGCTGAGTC TACGATTGAA AGTAAGAATT
    CTGACATGCT GCTGTTGCAA AATAACTTGA AAGAACTTGA GGAGTTAAGA
    GAAATGAAAG AGGACATTGA CAGAAAAAAT GAGCAAACAG CTGCCATTTT
    GAAAATGCAA GGAGCCCAAC TTGCTGAGCT AGAAATACTT TATAAGGAAG
    AACAAGTTTT AAGGAAAAGA TACTATAATA CCATAGAAGA TATGAAAGGG
    AAGATTAGAG TTTATTGTCG AATAAGACCT CTAAATGAAA AAGAGAGTTC
    AGAGAGGGAA AAACAAATGC TGACAACTGT GGATGAGTTT ACTGTTGAAC
    ATCCATGGAA AGACGACAAA AGAAAGCAAC ACATATATGA TCGCGTATTT
    GACATGCGTG CTAGTCAAGA TGATATCTTT GAAGACACAA AGTATTTGGT
    ACAGTCGGCT GTAGATGGGT ATAACGTTTG CATCTTTGCA TATGGTCAAA
    CTGGTTCTGG AAAAACTTTC ACTATATATG GGCATGAGAG CAATCCTGGA
    CTCACACCTC GAGCTACAAA GGAACTGTTC AACATATTAA AGCGAGATAG
    CAAGAGATTT TCATTTTCTC TAAAGGCATA TATGGTGGAA CTTTATCAAG
    ACACACTTGT AGACCTTTTG TTACCAAAAA GTGCAAGACG CTTGAAACTA
    GAGATTAAAA AAGATTCAAA GGGAATGGTC TTTGTAGAGA ATGTGACAAC
    TATTCCTATA TCAACTTTGG AGGAACTGCG AATGATTCTT GAACGGGGAT
    CGGAACGACG ACATGTTTCT GGAACAAATA TGAATGAAGA AAACTCAAGA
    TCTCACCTCA TATTATCAGT TGTTATTGAA AGTATTGATC TTCAAACCCA
    GTCTGCTGCG AGGGGCAAGC TGAGTTTTGT GGATCTTGCT GGTTCTGAGA
    GAGTTAAAAA GTCGGGCTCA GCTGGTTGTC AACTCAAAGA AGCTCAAAGT
    ATCAACAAAT CACTTTCTGC ATTAGGTGAT GTTATTGGTG CTTTATCTTC
    TGGCAACCAG CATATTCCTT ATAGGAATCA CAAGCTAACG ATGTTGATGA
    GCGATTCATT GGGCGGCAAT GCCAAGACGT TAATGTTTGT TAATGTGTCT
    CCAGCCGAAT CAGATTTGGA CGAGACGTAC AATTCTCTTC TATATGCATC
    GAGAGTGAGA ACGATCGTGA ATGATCCCAG CAAACATATA TCATCCAAAG
    AGATGGTGCG ATTGAAGAAG TTGGTAGCAT ACTGGAAAGA GCAAGCCGGT
    AAAAAAGGTG AGGAAGAAGA CTTGGTGGAT ATTGAGGAAG ATCGTACACG
    AAAAGATGAG GCAGATAGTT GAAGAAAGCT GACATGAAAT TCGTCTAGGT
    GAAATTGAGA CTATAGTATC ACTGGTTTTC ACCATTCTTT TTGTAGGAAG
    TTCCTGCTTT ATAGTAGAGT TTCTATCGAA GAGTAGTCCT ATATGTCTCA
    AACTTTCTCT CTTTTGATGA GATCTTCATT ACAAGAAATG AAAAAAAAAA
    AAAAAATTCG TAAAAAAAAA AAAAAAA

    Gene models with high sequence identity

    1. AT5G65930.2 [+] show detail - kinesin-like calmodulin-binding protein (ZWICHEL)
      E-value: 0; Score: 99.90

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan FERM domain 34.923
    ProfileScan Kinesin, motor domain 51.459
    ProfileScan MyTH4 domain 51.607
    Gene3D FERM/acyl-CoA-binding protein, 3-helical bundle 1.2e-20
    Gene3D Kinesin, motor domain 4.4e-119
    HMMPfam Kinesin, motor domain 2.8e-103
    HMMPfam MyTH4 domain 3.7e-39
    HMMPfam FERM central domain 3.1e-11
    HMMPfam FERM, N-terminal 1.2e-08
    Coil coiled-coil 0
    Coil coiled-coil 0
    superfamily P-loop containing nucleoside triphosphate hyd 3.8e-117
    superfamily FERM central domain 2.5e-21
    superfamily Prismane-like 9e-09
    superfamily PH domain-like 1.9e-06
    PatternScan Kinesin, motor region, conserved site 0
    HMMPanther CALMODULIN BINDING PROTEIN 3.1e-284
    HMMPanther KINESIN HEAVY CHAIN 3.1e-284
    FPrintScan Kinesin, motor domain 2.1e-38
    FPrintScan Kinesin, motor domain 2.1e-38
    FPrintScan Kinesin, motor domain 2.1e-38
    FPrintScan Kinesin, motor domain 2.1e-38
    HMMSmart MyTH4 domain 1.1e-51
    HMMSmart Band 4.1 domain 7.6e-38
    HMMSmart Kinesin, motor domain 3e-162

    See the detailed result >

    AT5G65930.2

    Model type
    Protein coding
    Short Description
    kinesin-like calmodulin-binding protein (ZWICHEL)
    Curator Summary
    encodes a novel member of the kinesin superfamily of motor proteins. recessive mutations have reduced number of trichome branches.
    Computational Description
    ZWICHEL (ZWI); FUNCTIONS IN: microtubule motor activity, calmodulin binding; INVOLVED IN: trichome branching, pollen germination; LOCATED IN: plasma membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: FERM central domain (InterPro:IPR019748), Prismane-like (InterPro:IPR011254), MyTH4 domain (InterPro:IPR000857), Kinesin, motor domain (InterPro:IPR001752), FERM, N-terminal (InterPro:IPR018979), Band 4.1 domain (InterPro:IPR019749), Kinesin, motor region, conserved site (InterPro:IPR019821), FERM/acyl-CoA-binding protein, 3-helical bundle (InterPro:IPR014352), FERM domain (InterPro:IPR000299); BEST Arabidopsis thaliana protein match is: Di-glucose binding protein with Kinesin motor domain (TAIR:AT2G22610.1); Has 92267 Blast hits to 53660 proteins in 2386 species: Archae - 1115; Bacteria - 10663; Metazoa - 44616; Fungi - 7060; Plants - 5081; Viruses - 192; Other Eukaryotes - 23540 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR