F18334

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F18334

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Original: F18334

Determination of introduced cDNA(s)

  • RAFL07-11-A11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL07-11-A11 - Resource: pda04115

    full reading [+] show sequence

    Nucleotide sequence: RAFL07-11-A11 - full reading
    AAAATTCACT CAACTTTCTC TCACTCCTTT TTTCTCTCTC TCTCTCTATC 
    CTCTACAGAG CTCGCAGCAA CTAGCGCAGG TACTCTCTCT TTCTCACTCC
    TTAATCTAGA TACTAAAGCT GATCATTTTC ATATCTCTCA GTAATCCGTT
    TCGTCTGATC GGGATGTTGG AGAAGGTTGA AACTTTCGAC ATGAACAGAG
    TCATTGATGA ATTCGATGAA ATGACTAGAA ACGCTCATCA AGTCCAGAAA
    CAAACTTTGA AAGAGATTCT TCTCAAGAAC CAATCCGCCA TTTACTTACA
    AAACTGTGGT CTCAATGGAA ACGCTACTGA CCCTGAAGAA GCTTTCAAAT
    CTATGGTTCC TTTAGTCACT GATGTTGAAT TAGAGCCTTA CATCAAAAGA
    ATGGTTGATG GTGACACTTC ACCTATTCTC ACTGGTCACC CTGTTCCTGC
    CATTTCCTTA AGCTCTGGAA CTAGTCAAGG CCGTCCAAAG TTTATTCCTT
    TCACTGATGA ATTAATGGAG AACACATTGC AACTGTTTCG CACTGCTTTT
    GCCTTCAGAA ACAGAGATTT TCCCATTGAT GACAATGGCA AAGCTTTGCA
    ATTTATCTTC AGCAGCAAGC AGTACATATC AACGGGAGGT GTCCCGGTTG
    GAACCGCAAC CACAAACGTG TACCGCAACC CTAACTTCAA AGCTGGGATG
    AAATCTATAA CGTCTCCTTC CTGTAGTCCC GACGAAGTTA TCTTTAGTCC
    TGATGTCCAT CAAGCCTTGT ATTGCCATCT CTTATCCGGA ATTCTCTTCA
    GAGATCAAGT TCAATACGTC TTTGCCGTCT TTGCTCACGG TCTAGTTCAC
    GCTTTTAGAA CCTTTGAACA GGTTTGGGAA GAGATTGTTA CCGATATCAA
    AGATGGTGTC TTAAGCAACC GTATTACTGT CCCATCGGTC CGTACTGCAA
    TGTCGAAGCT GCTCACACCT AACCCTGAGC TAGCTGAGAC GATCCGCACC
    AAATGTATGA GTTTGAGCAA CTGGTACGGA TTGATTCCCG CGCTCTTCCC
    CAACGCAAAG TATGTTTACG GGATCATGAC TGGCTCCATG GAGCCGTATG
    TGCCAAAGCT GAGACATTAC GCGGGTGATC TACCTCTTGT GAGCCATGAC
    TACGGTAGCT CGGAAGGATG GATTGCTGCT AATGTTACCC CAAGGTTATC
    TCCAGAGGAA GCTACATTTG CTGTGATTCC GAATCTCGGT TATTTCGAGT
    TTCTCCCTGT ATCTGAAACA GGGGAAGGAG AGGAGAAACC GGTTGGTTTA
    ACTCAAGTCA AGATTGGAGA AGAGTATGAA GTTGTCATCA CAAATTACGC
    AGGGTTGTAT CGATACCGGC TTGGAGATGT GGTAAAGGTC ATTGGTTTCT
    ACAACAACAC TCCCCAACTA AAATTCATAT GCAGGAGAAA CCTGATTCTC
    TCGATCAACA TCGACAAGAA CACCGAAAGA GACCTTCAGC TTTCAGTAGA
    ATCGGCTGCA AAGAGACTCT CAGAAGAAAA GATCGAGGTC ATAGACTTCT
    CTAGCTACAT CGATGTCTCG ACAGATCCAG GACATAACGC TATCTTCTGG
    GAGATTTCAG GAGAAACAAA CGAAGACGTT CTTCAAGATT GCTGCAACTG
    TTTAGACCGA GCATTCATCG ATGCGGGTTA CGTTAGTTCC CGGAAATGCA
    AGACTATTGG AGCCTTGGAG CTGAGAGTTG TCGCAAAAGG AACTTTCCGG
    AAGATTCAAG AACATTTTCT CGGGCTTGGT TCATCGGCTG GACAGTTTAA
    GATGCCAAGA TGTGTGAAGC CAAGTAATGC TAAGGTTCTC CAAATTCTGT
    GTGAGAATGT GGTGAGTAGC TACTTCAGCA CAGCGTTTTG ACTCACTAAA
    ATTGTGTGAA ATCAAAGAAA GTGGTTTTTA GTTGTTTATT TCCGTCGTTG
    TTGTCTTTAT TTGTCAATAT AGTTTTCTTT ACGTTATTTA ATTGAGCATA
    AACTTTTTTT ATCTCAAAAA AAAAAAAAAA A

    Gene models with high sequence identity

    1. AT2G46370.1 [+] show detail - Auxin-responsive GH3 family protein
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    Coil coiled-coil 0
    HMMPfam GH3 auxin-responsive promoter 1.6e-178

    See the detailed result >

    AT2G46370.1

    Model type
    Protein coding
    Short Description
    Auxin-responsive GH3 family protein
    Curator Summary
    Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.
    Computational Description
    JASMONATE RESISTANT 1 (JAR1); FUNCTIONS IN: catalytic activity, adenylyltransferase activity, ATP binding, jasmonate-amino synthetase activity; INVOLVED IN: in 13 processes; LOCATED IN: vacuole, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: GH3 auxin-responsive promoter (InterPro:IPR004993); BEST Arabidopsis thaliana protein match is: Auxin-responsive GH3 family protein (TAIR:AT4G03400.1); Has 1444 Blast hits to 1399 proteins in 231 species: Archae - 0; Bacteria - 474; Metazoa - 52; Fungi - 2; Plants - 663; Viruses - 0; Other Eukaryotes - 253 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR