F19129

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19129

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19129

Determination of introduced cDNA(s)

  • RAFL11-01-E14 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL11-01-E14 - Resource: pda10048

    full reading [+] show sequence

    Nucleotide sequence: RAFL11-01-E14 - full reading 
    GGGAGGCACA CATTACACAC CAAAGCAGTG TTTTCTTTCT CCTGAAAAGA 
    TTTTTCAAGC TCTCTCTTTC TCTCAAAGGT AAATGCTTCT CTGATTTCGC 
    CATTTTTGCT TACTCGATTC AAAAGACATG TCGCCGGCAG CTACGGAGAC 
    GGAGCGATGG TGCGTTGTTA CCGGCGGTAG AGGATTCGCG GCGAGGCATC 
    TTGTTGAAAT GCTCGTACGC TACGAAATGT TTTGCGTCCG TATCGCCGAT 
    TTAGCTCCGG CGATAATGCT TGATCCTCAG GAAGGGAACG GAGTTCTCGA 
    CGAAGGATTA AGATCTGGTC GTGTTCAATA TATCTCCGCT GATCTTCGAG 
    ATAAATCTCA AGTCGTTAAA GCTTTTCAGG GAGCGGAGGT GGTGTTTCAT 
    ATGGCAGCTC CAGATTCTTC TATTAACAAT CATCAGCTTC AGTATTCGGT 
    GAATGTTCAA GGGACACAAA ATGTGATTGA TGCTTGTGTT GATGTTGGAG 
    TAAAGAGGCT TATTTACACT AGCTCTCCTA GTGTTGTGTT TGATGGTGTT 
    CATGGTATTT TGAACGGTAC TGAATCAATG GCTTACCCAA TTAAGCACAA 
    TGACTCATAT TCAGCAACTA AAGCTGAAGG AGAAGAGTTG ATCATGAAGG 
    CTAATGGTAG AAATGGACTA CTCACTTGTT GCATACGTCC AAGCAGTATT 
    TTTGGTCCCG GTGATAGATT ATTGGTTCCG TCGCTTGTTG CTGCTGCTAG 
    GGCTGGGAAA TCTAAGCTTC AGGAGGAGAT GTTTCTACAA AAGCTGCAGG 
    ACAGTGGATT TACAGGCATA TTTCATTACC AACATGGAGC CAATTAAATT 
    TTGGGAATTT ATGTCACAGC TCCTTGATGG ACTTGGTTAC GAGAGGCCAA 
    GCATAAAGAT CCCTGCGTTT ATCATGATGC CGATTGCGCA TCTGGTTGAA 
    CTGACATATA AAGTGTTGGG ACCATATGGG ATGACAGTAC CACAACTAAC 
    ACCTTCAAGA GTTAGGCTTC TCTCATGCAG CAGAACTTTC GATTCCACGA 
    AAGCAAAGGA TCGACTAGGC TATGCTCCTG TGGTCCCACT TCAGGAAGGT 
    ATACGGAGGA CGATAGACTC ATTCTCTCAC CTAACAGCTG GAAGTCAATC 
    CAAAAGAGAG GGCCCATCCA AGGCTTCTAG GATTCTTGGA GGCGGAAAGG 
    TTGCTGATAC ACTTCTCTGG AAGGATTTAA AGCAGACTCT TATTGCCATA 
    TTCATTCTAA TTAGTATCTA TTATAACTTT GTTGCAACTG GGTCTACCGT 
    CGTAACTGCT CTTTCGAAGG CTCTATTGGT AGCATCTGTA TTTTTGTTCC 
    TCCATGGCAT TCTACCAGAG AAAATATTTG GTTATACAGT TGAAAAGATT 
    CCTGCATCGC AATTTCACCT CTCAAAAGAC TCATCACATG ACCTCTCATT 
    GTCAGTGATC TCTTCATGGA ACACCACTGT AAAAGCTTTA AGATCTCTTT 
    GCCAGGGGAA TGATTGGAGC TTCTTTTTCA AGGTGGTCTT CGTTTTGTTA 
    GCATTGAGTT TGGCTGGAGC CATATCTCTT CACAGCATAT TTGTTATAGG 
    ACTGCCCATT GCCTTCCTTG CTTTTCTCGT GTACGAAAAG AAAGAGCAAG 
    AGATCGACTC AATAGTGGTG AGTTTTAAAT CTTTCGCATG CAAGCACAAA 
    TCCGATGTGT ATGAGAAATT GTTCGGAAGC AAGAAACATG ACTAAAAACC 
    CTTTTTTTGC CCAGCATTTG TAATTTTCTT TTGTTTCCAA CGACCTCTAA 
    CTCTGGTTTG AGCTTTTTTC AAGTTTTATT ATTTGTGAAC

    Gene models with high sequence identity

    1. AT2G26260.2 [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
      E-value: 0; Score: 98.96

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 3 BETA-HYDROXYSTEROID DEHYDROGENASE 2.6e-35
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 2.6e-35
    HMMPanther 3 BETA-HYDROXYSTEROID DEHYDROGENASE 4e-86
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 4e-86
    superfamily NAD(P)-binding domain 1.9e-40
    superfamily NAD(P)-binding domain 3.9e-11
    ProfileScan Reticulon 14.371
    HMMPfam 3-beta hydroxysteroid dehydrogenase/isomerase 3.7e-55
    HMMPfam Reticulon 3.3e-31
    HMMPfam 3-beta hydroxysteroid dehydrogenase/isomerase 0.00012
    Gene3D NAD(P)-binding domain 5.2e-34

    See the detailed result >

    AT2G26260.2

    Model type
    Protein coding
    Short Description
    3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
    Curator Summary
    Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
    Computational Description
    3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (3BETAHSD/D2); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 12 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; CONTAINS InterPro DOMAIN/s: Reticulon (InterPro:IPR003388), 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (TAIR:AT1G47290.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR