F19208

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19208

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
fruit (PO:0009001)
  • increased length (PATO:0000573)
    (Silique::Shape::long)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19208

Determination of introduced cDNA(s)

  • RAFL05-10-E23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-10-E23 - Resource: pda00913

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-10-E23 - full reading 
    AGGTGCCTCA AAAAATCATA ATTTTCTCGG AAAATTAAAA ATCATGTTTA 
    AGATTTTCTC GAGAAAGTAA AAAAAGGAAG AAGAAGAAGA AGAAACCATG 
    GGAAGAGCTT TCGTGTATGT AATTCTCGGA GGAGGTGTTG CTGCTGGTTA 
    CGCAGCTCTC GAATTCACCC GTCGAGGTGT CTCCGATGGC GAACTCTGTA 
    TCATTTCCGA AGAACCCGTT GCACCGTATG AGAGGCCTGC CTTAAGCAAA 
    GGTTTTCTGC TGCCAGAAGC TCCTGCACGG CTTCCTTCTT TCCACACTTG 
    TGTTGGAGCT AATGATGAGA AGCTTACACC GAAATGGTAC AAGGATCATG 
    GAATTGAATT GGTACTTGGA ACTCGTGTTA AGTCCGTTGA TGTGAGGAGG 
    AAGACTCTTT TATCGAGCAC AGGGGAGACT ATAAGTTACA AATTCTTGAT 
    CATTGCAACA GGTGCCCGGG CGTTGAAGCT TGAAGAATTT GGGGTGGAAG 
    GCTCAGATGC TGAAAATGTT TGTTACTTAC GAGATCTAGC TGATGCAAAT 
    AGGCTTGCCA CTGTGATTCA ATCAAGCTCC AACGGGAATG CGGTTGTTAT 
    CGGCGGTGGC TATATCGGTA TGGAGTGTGC TGCATCTTTA GTGATCAACA 
    AAATCAATGT GACGATGGTT TTCCCAGAGG CTCACTGCAT GGCACGTCTC 
    TTTACGCCCA AGATTGCAAG TTTGTATGAA GACTACTACA GGGCTAAAGG 
    CGTAAAATTT ATAAAAGGAA CGGTTTTGAC ATCATTTGAG TTTGACTCGA 
    ACAAAAAGGT TACTGCAGTT AATCTCAAAG ACGGGAGCCA CTTACCAGCC 
    GACTTGGTTG TGGTTGGTAT CGGGATACGA CCAAACACAA GCTTGTTTGA 
    AGGACAGTTA ACAATAGAGA AAGGAGGGAT AAAAGTGAAC AGCAGAATGC 
    AGTCAAGTGA CAGCTCAGTG TATGCAATAG GCGATGTAGC TACATTTCCA 
    GTGAAACTAT TTGGCGAAAT GAGGAGGCTT GAGCACGTGG ACTCAGCTAG 
    GAAATCTGCG CGACACGCAG TTTCAGCCAT CATGGATCCA ATAAAGACCG 
    GAGACTTTGA TTACTTACCA TTTTTCTACT CAAGGGTCTT CGCATTCTCA 
    TGGCAATTCT ATGGAGACCC TACAGGTGAT GTTGTGCACT TTGGAGAGTA 
    TGAAGACGGG AAATCATTTG GAGCGTATTG GGTTAAAAAG GGTCACCTTG 
    TTGGATCTTT TCTTGAAGGA GGCACTAAAG AAGAATATGA AACTATCTCA 
    AAGGCGACAC AATTGAAACC AGCTGTGACT ATTGATTTGG AAGAATTGGA 
    AAGAGAAGGA CTCGGGTTTG CTCACACGGT TGTGAGTCAG CAGAAAGTTC 
    CTGAAGTTAA GGATATTCCG AGCGCTGAGA TGGTGAAGCA ATCTGCAAGT 
    GTGGTCATGA TAAAGAAGCC TTTGTATGTA TGGCACGCAG CTACTGGAGT 
    CGTTGTGGCT GCATCTGTGG CTGCGTTCGC GTTTTGGTAT GGGAGGAGAC 
    GCCGTAGATG GTGAGGAAAA AAAAAACTCT TCAAGTGTTG GATTCATTGG 
    AAATGGAACT TGCTGTTGTT TGTAGCATTG GTGGGAGAAA TATAGAAGGG 
    AGAGTCCAAA CTACTCAATG TGATTACATT GGTATACGAT TATTATATGC 
    TTCTTTCAAA AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT3G27820.1 [+] show detail - monodehydroascorbate reductase 4
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther MONODEHYDROASCORBATE REDUCTASE (NADH) 5.5e-167
    HMMPanther DISULFIDE OXIDOREDUCTASE 5.5e-167
    superfamily FAD/NAD(P)-binding domain 5.8e-42
    superfamily FAD/NAD(P)-binding domain 5.7e-20
    superfamily FAD/NAD-linked reductase, dimerisation 1.4e-13
    Gene3D no description 6.5e-21
    Gene3D no description 1.2e-38
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
    FPrintScan FAD-dependent pyridine nucleotide-disulphide oxidoreductase 6.7e-21
    FPrintScan PNDRDTASEI 8.8e-14
    FPrintScan PNDRDTASEI 8.8e-14
    FPrintScan PNDRDTASEI 8.8e-14
    FPrintScan PNDRDTASEI 8.8e-14
    HMMPfam FAD-dependent pyridine nucleotide-disulphide oxidoreductase 5.4e-38
    HMMPfam Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region 4.2e-15

    See the detailed result >

    AT3G27820.1

    Model type
    Protein coding
    Short Description
    monodehydroascorbate reductase 4
    Curator Summary
    Encodes a peroxisome membrane-bound monodehydroascorbate reductase, involved in the ascorbate-glutathione cycle which removes toxic H2O2
    Computational Description
    monodehydroascorbate reductase 4 (MDAR4); FUNCTIONS IN: monodehydroascorbate reductase (NADH) activity; INVOLVED IN: hydrogen peroxide catabolic process; LOCATED IN: peroxisomal membrane, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: FAD/NAD-linked reductase, dimerisation (InterPro:IPR016156), FAD-dependent pyridine nucleotide-disulphide oxidoreductase (InterPro:IPR013027), Pyridine nucleotide-disulphide oxidoreductase, NAD-binding region (InterPro:IPR001327); BEST Arabidopsis thaliana protein match is: monodehydroascorbate reductase 1 (TAIR:AT3G52880.1); Has 27219 Blast hits to 27159 proteins in 2894 species: Archae - 579; Bacteria - 21951; Metazoa - 737; Fungi - 533; Plants - 600; Viruses - 0; Other Eukaryotes - 2819 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR