F21319

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F21319

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F21319

Determination of introduced cDNA(s)

  • RAFL09-07-H17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-07-H17 - Resource: pda05177

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-07-H17 - full reading 
    GAGAGCTAAG ATTTTCCCAT CAACTCCATT TGAAGCGAAA TTCTCTGATC 
    TCTCTTCATT TTATTTTCCC TCTACATCTC TCCCAACGAT TTCCAAATCT 
    CACTTCACTT TGGCTCCTCC ACCTGAATCC ATGGATATTG CTTCGTCATC 
    TCTCTCACAA GCTCACAAAG TCGTTCTCAC GCGTCAACCT TCTTCCCGGG 
    TCAACACCTG CTCCCTTGGC TCGGTCTCCG CTATCGGATT CTCTCTTCCG 
    CAGATTAGCT CTCCAGCTTT AGGTAAATGT CGCCGGAAAC AAAGCTCTTC 
    TGGGTTCGTG AAAGCTTGTG TTGCTGTTGA ACAGAAAACC CGAACTGCTA 
    TCATCAGAAT TGGCACAAGG GGAAGTCCTC TAGCACTTGC TCAAGCATAC 
    GAGACGCGAG AAAAGCTCAA GAAGAAACAC CCTGAACTCG TTGAAGATGG 
    AGCTATTCAT ATCGAGATCA TTAAAACGAC TGGTGATAAG ATTCTTTCGC 
    AACCGCTTGC TGATATTGGT GGGAAAGGAC TTTTCACCAA AGAAATAGAC 
    GAGGCCTTGA TAAATGGTCA TATTGACATA GCTGTGCACT CAATGAAAGA 
    TGTCCCAACT TACTTACCAG AAAAAACGAT TTTACCTTGT AACCTTCCGC 
    GTGAGGATGT TCGAGATGCG TTTATTTGTC TAACTGCAGC CACGTTAGCT 
    GAGCTTCCAG CTGGAAGCGT TGTGGGAACA GCTTCTCTCA GGAGAAAATC 
    GCAGATTCTC CACAAATATC CTGCATTACA TGTTGAGGAA AACTTCAGGG 
    GTAATGTGCA GACAAGACTA TCAAAACTAC AAGGAGGAAA GGTCCAAGCA 
    ACTCTATTAG CACTAGCTGG TCTTAAGAGA TTGAGTATGA CAGAGAATGT 
    CGCATCTATC TTATCTCTCG ATGAAATGCT TCCAGCTGTT GCTCAAGGAG 
    CTATTGGAAT TGCCTGTAGA ACTGATGATG ATAAAATGGC AACTTACTTA 
    GCCTCACTGA ACCACGAGGA AACAAGACTA GCGATTTCAT GCGAGAGAGC 
    TTTTCTTGAA ACGCTAGATG GCTCATGCCG TACTCCTATT GCTGGATACG 
    CATCCAAGGA CGAAGAAGGC AACTGCATTT TCAGAGGATT GGTTGCTTCC 
    CCTGACGGTA CTAAAGTTCT TGAGACCTCA AGAAAAGGTC CATACGTGTA 
    TGAAGACATG GTGAAGATGG GAAAAGACGC GGGGCAAGAA TTGCTATCTC 
    GTGCTGGTCC TGGCTTCTTC GGCAACTGAG CCATTCGAAG AAGAGGAGGA 
    AGAGATGTGA CACGGACAAA GTCTAGTGTT TAGGAGTGAT TCAACATCTT 
    GAAGTCCCGT TTCTTCCTCC GAACTGTTGT AACTTTTTTG ATTTTGAGAG 
    AAACATGACC TTTTGTTTAT TGATTGTTTT GTTGTATTTA GATTAAATTC 
    ATCTTGCAAT TGTACCCC

    Gene models with high sequence identity

    1. AT5G08280.1 [+] show detail - hydroxymethylbilane synthase
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Periplasmic binding protein-like II 1.1e-76
    superfamily Porphobilinogen deaminase, C-terminal domain 1.2e-20
    PatternScan Porphobilinogen deaminase, dipyrromethane cofactor binding site 0
    HMMPfam Porphobilinogen deaminase, N-terminal 2.1e-81
    HMMPfam Porphobilinogen deaminase, C-terminal domain 4.7e-19
    FPrintScan Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 7.7e-44
    FPrintScan Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 7.7e-44
    FPrintScan Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 7.7e-44
    FPrintScan Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 7.7e-44
    FPrintScan Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 7.7e-44
    HMMTigr Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 2e-130
    HMMPanther Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 8.2e-73
    Gene3D no description 4.5e-37
    Gene3D Porphobilinogen deaminase, C-terminal domain 1.1e-16
    HAMAP Tetrapyrrole biosynthesis, hydroxymethylbilane synthase 33.756

    See the detailed result >

    AT5G08280.1

    Model type
    Protein coding
    Short Description
    hydroxymethylbilane synthase
    Curator Summary
    Encodes a protein with porphobilinogen deaminase activity. This protein is targeted to the chloroplast.
    Computational Description
    hydroxymethylbilane synthase (HEMC); FUNCTIONS IN: hydroxymethylbilane synthase activity; INVOLVED IN: chlorophyll biosynthetic process, defense response to bacterium, porphyrin biosynthetic process; LOCATED IN: apoplast, chloroplast, chloroplast stroma, chloroplast envelope; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Porphobilinogen deaminase, dipyrromethane cofactor binding site (InterPro:IPR022419), Tetrapyrrole biosynthesis, hydroxymethylbilane synthase (InterPro:IPR000860), Porphobilinogen deaminase, N-terminal (InterPro:IPR022417), Porphobilinogen deaminase, C-terminal domain (InterPro:IPR022418); Has 7343 Blast hits to 7329 proteins in 2247 species: Archae - 198; Bacteria - 4150; Metazoa - 157; Fungi - 178; Plants - 78; Viruses - 0; Other Eukaryotes - 2582 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR