F22110

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F22110

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22110

Determination of introduced cDNA(s)

  • RAFL04-13-F08 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-13-F08 - Resource: pda00355

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-13-F08 - full reading 
    GAAAGAAACC CACAGCAACA AACAGAGAAA ATGTGTGGCG GTGCTATTAT 
    TTCCGATTAT GCCCCTCTCG TCACCAAGGC CAAGGGCCGT AAACTCACGG 
    CTGAGGAACT CTGGTCAGAG CTCGATGCTT CCGCCGCCGA CGACTTCTGG 
    GGTTTCTATT CCACCTCCAA ACTCCATCCC ACCAACCAAG TTAACGTGAA 
    AGAGGAGGCA GTGAAGAAGG AGCAGGCAAC AGAGCCGGGG AAACGGAGGA 
    AGAGGAAGAA TGTTTATAGA GGGATACGTA AGCGTCCATG GGGAAAATGG 
    GCGGCTGAGA TTCGAGATCC ACGAAAAGGT GTTAGAGTTT GGCTTGGTAC 
    GTTCAACACG GCGGAGGAAG CTGCCATGGC TTATGATGTT GCGGCCAAGC 
    AGATCCGTGG TGATAAAGCC AAGCTCAACT TCCCAGATCT GCACCATCCT 
    CCTCCTCCTA ATTATACTCC TCCGCCGTCA TCGCCACGAT CAACCGATCA 
    GCCTCCGGCG AAGAAGGTCT GCGTTGTCTC TCAGAGTGAG AGCGAGTTAA 
    GTCAGCCGAG TTTCCCGGTG GAGTGTATAG GATTTGGAAA TGGGGACGAG 
    TTTCAGAACC TGAGTTACGG ATTTGAGCCG GATTATGATC TGAAACAGCA 
    GATATCGAGC TTGGAATCGT TCCTTGAGCT GGACGGTAAC ACGGCGGAGC 
    AACCGAGTCA GCTTGATGAG TCCGTTTCCG AGGTGGATAT GTGGATGCTT 
    GATGATGTCA TTGCGTCGTA TGAGTAAAAG AAAAAAAATA AGTTTAAAAA 
    AAGTTAAATA AAGTCTGTAA TATATATGTA ACCGCCGTTA CTTTTAAAAG 
    GTTTTTACCG TCGCATTGGA CTGCTGATGA TGTCTGTTGT GTAATGTGTA 
    GAATGTGACC AAATGGACGT TATATTACGG TTTGTGGTAA AGAAAAAAAA 
    AAAAAAA

    Gene models with high sequence identity

    1. AT3G16770.1 [+] show detail - ethylene-responsive element binding protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Pathogenesis-related transcriptional factor/ERF, DNA-binding 1.7e-11
    FPrintScan Pathogenesis-related transcriptional factor/ERF, DNA-binding 1.7e-11
    HMMPfam Pathogenesis-related transcriptional factor/ERF, DNA-binding 1.6e-16
    Gene3D Pathogenesis-related transcriptional factor/ERF, DNA-binding 1.5e-23
    superfamily DNA-binding, integrase-type 1.3e-24
    ProfileScan Pathogenesis-related transcriptional factor/ERF, DNA-binding 24.171
    HMMSmart Pathogenesis-related transcriptional factor/ERF, DNA-binding 1.1e-36

    See the detailed result >

    AT3G16770.1

    Model type
    Protein coding
    Short Description
    ethylene-responsive element binding protein
    Curator Summary
    Encodes a member of the ERF (ethylene response factor) subfamily B-2 of the plant specific ERF/AP2 transcription factor family (RAP2.3). The protein contains one AP2 domain. There are 5 members in this subfamily including RAP2.2 AND RAP2.12.It is localized to the nucleus and acts as a transcriptional activator through the GCC-box. It has been identified as a suppressor of Bax-induced cell death by functional screening in yeast and can also suppress Bax-induced cell death in tobacco plants. Overexpression of this gene in tobacco BY-2 cells confers resistance to H2O2 and heat stresses. Overexpression in Arabidopsis causes upregulation of PDF1.2 and GST6. It is part of the ethylene signaling pathway and is predicted to act downstream of EIN2 and CTR1, but not under EIN3.
    Computational Description
    ethylene-responsive element binding protein (EBP); CONTAINS InterPro DOMAIN/s: DNA-binding, integrase-type (InterPro:IPR016177), Pathogenesis-related transcriptional factor/ERF, DNA-binding (InterPro:IPR001471); BEST Arabidopsis thaliana protein match is: Integrase-type DNA-binding superfamily protein (TAIR:AT2G47520.1); Has 5957 Blast hits to 5752 proteins in 250 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 5929; Viruses - 4; Other Eukaryotes - 24 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR