F23647

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23647

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23647

Determination of introduced cDNA(s)

  • RAFL05-10-A17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-10-A17 - Resource: pda17041

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-10-A17 - full reading 
    CCCGAAGTTT TCGACAGATC AACTGTTTTC TTCATTACGT GTTCTTCAAT 
    GGCGTCACTT CACTGATTCT ATTCCTATCA AACCCACTTA CCCATTCCCT 
    TTTATATGCA CAAATTCTCA TAAAGTTTTC AATTTTATTC CATTTTTCTC 
    GGAAGCCATG GAAGCTGCTA ATGAGCCTGT TAATGGCGGA TCCGTACAGA 
    TCCGAACAGA GAACAACGAA AGACGAAAGC TTCCTAATTT CTTACAAAGC 
    GTCAACATGA AATACGTCAA GCTAGGTTAT CATTACCTCA TTACTCATCT 
    CTTCAAGCTC TGTTTGGTTC CATTAATGGC GGTTTTAGTC ACAGAGATCT 
    CTCGATTAAC AACAGACGAT CTTTACCAGA TTTGGCTTCA TCTCCAATAC 
    AATCTCGTTG CTTTCATCTT TCTCTCTGCT TTAGCTATCT TTGGCTCCAC 
    CGTTTACATC ATGAGTCGTC CCAGATCTGT TTATCTCGTT GATTACTCTT 
    GTTATCTTCC TCCGGAGAGT CTTCAGGTTA AGTATCAGAA GTTTATGGAT 
    CATTCTAAGT TGATTGAAGA TTTCAATGAG TCATCTTTAG AGTTTCAGAG 
    GAAGATTCTT GAACGTTCTG GTTTAGGAGA AGAGACTTAT CTCCCTGAAG 
    CTTTACATTG TATCCCTCCG AGGCCTACGA TGATGGCGGC TCGTGAGGAA 
    TCTGAGCAGG TAATGTTTGG TGCTCTTGAT AAGCTTTTCG AGAATACCAA 
    GATTAACCCT AGGGATATTG GTGTGTTGGT TGTGAATTGT AGCTTGTTTA 
    ATCCTACACC TTCGTTGTCA GCTATGATTG TTAACAAGTA TAAGCTTAGA 
    GGGAATGTTA AGAGTTTTAA CCTTGGTGGA ATGGGGTGTA GTGCTGGTGT 
    TATCTCTATC GATTTAGCTA AAGATATGTT GCAAGTTCAT AGGAATACTT 
    ATGCTGTTGT GGTTAGTACT GAGAACATTA CTCAGAATTG GTATTTTGGG 
    AATAAGAAGG CTATGTTGAT TCCGAATTGT TTGTTTCGTG TTGGTGGTTC 
    GGCGATTTTG TTGTCGAACA AGGGGAAAGA TCGTAGACGG TCTAAGTATA 
    AGCTTGTTCA TACCGTTAGG ACTCATAAAG GAGCTGTTGA GAAGGCTTTC 
    AACTGTGTTT ACCAAGAGCA AGATGATAAT GGGAAGACCG GGGTTTCGTT 
    GTCGAAAGAT CTTATGGCTA TAGCTGGGGA AGCTCTTAAG GCGAATATCA 
    CTACTTTAGG TCCTTTGGTT CTTCCTATAA GTGAGCAGAT TCTGTTTTTC 
    ATGACTTTGG TTACGAAGAA ACTGTTTAAC TCGAAGCTGA AGCCGTATAT 
    TCCGGATTTC AAGCTTGCGT TTGATCATTT CTGTATCCAT GCTGGTGGTA 
    GAGCTGTGAT TGATGAGCTT GAGAAGAATC TGCAGCTTTC GCAGACTCAT 
    GTCGAGGCAT CCAGAATGAC ACTGCACAGA TTTGGAAACA CTTCTTCGAG 
    CTCGATTTGG TATGAACTGG CTTACATAGA GGCTAAAGGT AGGATGAAGA 
    AAGGAAACCG GGTTTGGCAG ATTGCTTTTG GAAGTGGGTT TAAGTGTAAC 
    AGTGCAGTTT TGGGTGGCTC TAAACAATGT CAAGCCTTCG GTTAGTAGTC 
    CGTGGGAACA CTGCATCGAC CGATATCCGG TTAAGCTCGA CTTCTGATTT 
    AATGAAAAAC CATTTGTTTG GTTAGTCCTA GTCTTACTAG ACTCTGCCTT 
    GTTTATTTTT CTTCTTTTCT GGTTAAGCTC TTGTACTCTT AATGTGACGC 
    TTCTTGATTA TTACTCTCGC GTTTTATTCT ATGGCATCTT TATCCAAAAA 
    AAAAA

    Gene models with high sequence identity

    1. AT2G16280.1 [+] show detail - 3-ketoacyl-CoA synthase 9
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Thiolase-like 2.3e-95
    HMMPfam FAE1/Type III polyketide synthase-like protein 1.7e-146
    HMMPfam 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal 3.4e-12
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMPIR Very-long-chain 3-ketoacyl-CoA synthase 0
    Gene3D Thiolase-like, subgroup 6.9e-55
    Gene3D Thiolase-like, subgroup 2.1e-24

    See the detailed result >

    AT2G16280.1

    Model type
    Protein coding
    Short Description
    3-ketoacyl-CoA synthase 9
    Curator Summary
    Encodes KCS9, a member of the 3-ketoacyl-CoA synthase family involved in the biosynthesis of VLCFA (very long chain fatty acids).
    Computational Description
    3-ketoacyl-CoA synthase 9 (KCS9); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity; INVOLVED IN: response to cold, response to light stimulus, very long-chain fatty acid metabolic process, response to osmotic stress, cuticle development; LOCATED IN: membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 4 (TAIR:AT1G19440.1); Has 4705 Blast hits to 4688 proteins in 1155 species: Archae - 0; Bacteria - 1679; Metazoa - 0; Fungi - 8; Plants - 2880; Viruses - 0; Other Eukaryotes - 138 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR