F24236

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F24236

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F24236

Determination of introduced cDNA(s)

  • RAFL08-17-B19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL08-17-B19 - Resource: pdz87787

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL08-17-B19 - 5 prime EST 
    GAATATCTTC CTTTGTCAAT CTCTTGAGAG TTTTTGCTTC CTCGTCTTCT 
    TCGATTCATC AAACAGCGTG TGTTTTTTGT TTAGAGTTGG AAGAGACGAA 
    TCGAATTTTG AAGCTTTCTT TTATGTCTGA CAAGATATCT CCGTTTTGTT 
    GTCAATCTGG TTGCTTGGAA AACACAGTCC TACATCCAGA ATTAAGGACT 
    ACTACTAGTT CCTCCATCAC TTTAGGGTCT CGCATCTTCA AACCGGAGAT 
    CCCTCCTCTT TCTCTCCACT TTTTTAGCTA AGAAGAATGG CGCTGCAGAA 
    CATTGGTGCT TCCAACCGTA ACGATGCCTT CTACAGGTAC AAGATGCCTA 
    AGATGGTTAC CAAAACCGAA GGCAAAGGTA ATGGCATTAA GACCAACATT 
    ATCAACAATG TTGAGATTGC CAAAGCCTTG GCTAGACCGC CTTCTTATAC 
    GACCAAGTAC TTTGGTTGTG AGCTTGGAGC GCAGTCTAAG TTTGATGAGA 
    AGACTGGGAC GTCGCTTGTG AATGGAGCTC ACAACACGTC TAAGCTTGCT 
    GGGCTTTTGG AGAATTTTAT TAAGAAGTTT GTTCAGTGTT ATGGATGTGG 
    TAACCCCGGA GACTGAGATT ATTATTACA

    Gene models with high sequence identity

    1. AT1G77840.1 [+] show detail - Translation initiation factor IF2/IF5
      E-value: 0; Score: 99.84

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL08-17-B19 - 3 prime EST 
    AGCTTTTGTT CTCAATCGAT CAACTAAAAT TTCTCTGATA CACTTTTAAG 
    TTTCTAACAA AATTTCCTTT CAAAATAGAA TAAAAAAGTT TAAACACACT 
    TATCTCCTCT AACTTCTTCG AACGTGAGCT TCCTTTAGCA TTGTCTGCCA 
    AAGGTCTTTG ACACGATGAC GAAACTTTTT CTGAAACCAA ACTTCAATCT 
    CTGAATAAAG CACATTCGGA TTCTGTTCCC AATTCTTTCT CAAACTCTCC 
    AAAGTATCAA TCTTCTTCTC TGTCAAAACC ATCACCAAAC TCCCAACTCT 
    TTCTAAACCC CAAGCTTCTC TTTCCAACAA CGTTTCAGGT TTCCCAGCCA 
    ACAAAGCCCT GAAGCTTTTC AAACAAGTTA ATACCTCTCC TTGAAGCAAA 
    GCGCATCCGA AAGCCGCTGC CTCGGTCTCT ATCCT

    Gene models with high sequence identity

    1. AT1G33390.1 [+] show detail - RNA helicase family protein
      E-value: 0; Score: 99.77

    AT1G33390.1

    Model type
    Protein coding
    Short Description
    RNA helicase family protein
    Curator Summary
    Over-expression of this gene results in stem fasciation. The predicted amino acid sequence reveals the presence of two domains (DEXH-box or DEAD-box helicase and DUF1065 domain) and fragments of two more domains (HrpA domain and HA2 domain).
    Computational Description
    FASCIATED STEM 4 (FAS4); FUNCTIONS IN: helicase activity, ATP binding, ATP-dependent helicase activity, nucleic acid binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Helicase-associated domain (InterPro:IPR007502), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), Domain of unknown function DUF1605 (InterPro:IPR011709), DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site (InterPro:IPR002464), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: ATP-dependent RNA helicase, putative (TAIR:AT3G26560.1); Has 16056 Blast hits to 9082 proteins in 1556 species: Archae - 2; Bacteria - 6415; Metazoa - 3625; Fungi - 2171; Plants - 1181; Viruses - 334; Other Eukaryotes - 2328 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT1G77840.1

    Model type
    Protein coding
    Short Description
    Translation initiation factor IF2/IF5
    Computational Description
    Translation initiation factor IF2/IF5; FUNCTIONS IN: binding, translation initiation factor activity; INVOLVED IN: translational initiation, regulation of translational initiation; LOCATED IN: nucleus, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Translation initiation factor IF2/IF5, N-terminal (InterPro:IPR016189), Translation initiation factor IF2/IF5, zinc-binding (InterPro:IPR016190), eIF4-gamma/eIF5/eIF2-epsilon (InterPro:IPR003307), Translation initiation factor IF2/IF5 (InterPro:IPR002735), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: Translation initiation factor IF2/IF5 (TAIR:AT1G36730.1); Has 4597 Blast hits to 3869 proteins in 471 species: Archae - 198; Bacteria - 234; Metazoa - 1589; Fungi - 711; Plants - 391; Viruses - 12; Other Eukaryotes - 1462 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR