F25148

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F25148

Determination of introduced cDNA(s)

RAFL09-64-F03 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-64-F03 - Resource: pda08587

full reading [+] show sequence

AACAACCAGC TCAGGCCATA AGATTCATCC CGGAGAAACA AGAACGATGA 
CAACACATAA ACATAGAAGA ACAGAGAAAA ATCTCTGTTT TAAGCAATAC 
TACAAATGGA TCCTCTGTTT TATCCTCACT CTCTATTTTT TTGCTTCCTT 
CTTTGTCGAC CATGATCAAG ATCATAGATC TTCTACTTCC ATATCAAAGC 
ATCTTCTTAC TAACCATAAA CCTAAGCTTT TCGCTTCTCG TGCCATGTTT 
GAATCTAAAA TCCATGATCA CAAACTTGGG TTTACCTCTC AACAACCTAA 
CATCAAAACA GACGTATTCA ACAATTTGAA AATCTACGTC TATGACTTAC 
CTTCAAAGTT CAACAAAGAC TGGTTAGCCA ATGATAGATG CACCAACCAC 
CTCTTTGCCG CGGAAGTGGC TCTCCATAAG GCGTTTCTCA GCCTCGAAGG 
AGATGTAAGG ACGGAAGATC CGTATGAAGC CGATTTCTTC TTTGTCCCTG 
TCTATGTCTC TTGCAACTTC AGCACCATTA ATGGCTTTCC AGCGATTGGC 
CATGCACGAT CACTCATCAA TGACGCGATA AAGCTCGTCT CGACTCAATA 
CCCTTTTTGG AACCGAACCA GTGGCTCTGA TCATGTCTTC ACGGCCACAC 
ACGACTTTGG CTCTTGCTTC CACACCATGA GCTATTGCTG ATGGAGTACC 
AATTTTTTTG AGAAACTCTA TAATTTTGCA AACATTTGGT GTTACTTTTA 
ACCATCCATG CCAAGAGGTA GAGAACGTGG TGATACCACC GTACATCTCA 
CCGGAAAGTT TACACAAGAC TCAAAAGAAT ATTCCGGTAA CTAAAGAACG 
AGACATTTGG GTTTTTTTCC GTGGCAAGAT GGAACTTCAT CCCAAAAACA 
TCAGTGGACG CTTCTATAGC AAGCGTGTAA GGACGAATAT ATGGCGGAGT 
TACGGTGGTG ACCGGAGGTT TTATCTCCAA CGGCAAAGAT TTGCCGGTTA 
CCAGTCAGAA ATAGCTCGTT CTGTTTTCTG TTTATGTCCT CTCGGGTGGG 
CACCGTGGAG CCCAAGACTT GTTGAATCGG TGGCTCTTGG CTGTGTTCCG 
GTGATTATAG CCGACGGGAT TCGTTTGCCG TTCCCTTCCA CCGTACGTTG 
GCCGGATATT TCGCTCACGG TGGCGGAGAG AGACGTTGGA AAGCTAGGGG 
ATATTTTGGA ACACGTGGCG GCTACTAATT TGTCAGTCAT ACAGAGAAAC 
TTGGAGGATC CGTCTGTTAG GCGGGCCCTT ATGTTTAATG TTCCTTCAAG 
AGAGGGAGAT GCCACGTGGC AAGTTTTGGA GGCTTTGTCA AAGAAATTGA 
ATAGGTCTGT TAGAAGGTCA AACTCTTTCT TGTAAACCAA TATAATTTTG 
ACACCTGTGT ATTACTATTT TTTTTCCTGG CTAGTGAGAT ATAAGAGTAG 
TAAGCTCTTA AATGT

Gene models with high sequence identity

AT2G28110.1 [+] show detail - Exostosin family protein
E-value: 0; Score: 99.46

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Exostosin-like	6e-31
HMMPfam	Exostosin-like	2e-34
HMMPanther	EXOSTOSIN-RELATED	1.7e-39
HMMPanther	EXOSTOSIN (HEPARAN SULFATE GLYCOSYLTRANSFERAS	1.7e-39
HMMPanther	EXOSTOSIN-RELATED	4.8e-69
HMMPanther	EXOSTOSIN (HEPARAN SULFATE GLYCOSYLTRANSFERAS	4.8e-69

See the detailed result >

AT2G28110.1

Model type: Protein coding
Short Description: Exostosin family protein
Curator Summary: Homolog to AT5G22940, a member of glycosyltransferase family 47 that is involved in secondary cell wall biosynthesis. It exhibits high sequence similarity to tobacco (Nicotiana plumbaginifolia) pectin glucuronyltransferase. Protein has a domain that shares significant similarity with the pfam03016 domain. It is expressed specifically in developing vessels and fiber cells, and FRA8 is targeted to Golgi. Mutants have irregular xylem formation, reduced cellulose levels and plants are smaller than normal siblings.
Computational Description: FRAGILE FIBER 8 (FRA8); CONTAINS InterPro DOMAIN/s: Exostosin-like (InterPro:IPR004263); BEST Arabidopsis thaliana protein match is: FRA8 homolog (TAIR:AT5G22940.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link: InterPro Scan - TAIR