F27032

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27032

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27032

Determination of introduced cDNA(s)

  • RAFL04-17-I16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-I16 - Resource: pdz87414

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-17-I16 - 5 prime EST 
    GATTTCTCTG AAAACCCTTA AATCTCCGGA TTTCGATTCC CGAGGCTCGA 
    AAAAACCCTA AATCTCTTCT CCGTTCACTC ATCCAACCGA AGATGCGTGA 
    GATTCTTCAC ATCCAGGGNG GTCAATGCTG GTAACCAGAT CGGCGCCAAG 
    TTCTGGGAAG TGGTTTGCGC CGAGCACGGN ATCGATCCAA CCGGAAGGTA 
    CACCGGAGAC TCAGATCTGC AACTTGAGCG CATCAACGTT TACTACAATG 
    AAGCGAGTTG CGGTAGATTC GTTCCTCGTG CAGTGCTCAT GGATTTGGAG 
    CCTGGGACTA TGTATAGTCT CAGATCTGGA CCGTACGGTC AGACCTTTCG 
    ACCTGATAAC TTCGTCTTTG GCCAATCCGG TGCTGGTAAC AACTGGGCCA 
    AGGGACACCT ACACGGAAGG AGCTGAACTA ATCGATTCCG TTCTCGATGT 
    TGTTCGTAAG GAAGCTGANA ACTGTGGACT GTCTCCAAGG GTTCCAGGTT 
    GGTCA

    Gene models with high sequence identity

    1. AT5G62690.1 [+] show detail - tubulin beta chain 2
      E-value: 0; Score: 98.41

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-17-I16 - 3 prime EST 
    AACCAAACGC TTCTCTATTT TATTCATTAC AGAACCGCAC TTAACATATG 
    AAAATAGGGT TCACTGCACC AGATCATTAC CCACTAATCT CCTCTTAAAG 
    ACCTTGACAA AATTACATGT AATAATAATC AATCACTGCT TGTTGTTCAA 
    GAGAGCTGCT CGTAGCTCAG CCGCGTCAAT CTCGTTGGAA CCGGTTTCGG 
    GTCTGTAATA ACCGGTTTTG GGATCTGGAA CCCAAGAAAT CTTCTGGGTT 
    GATTCTTCCA CTCCCTTCTT CTTCATCACA GCCGAAGCAA CAGCTCCACT 
    TCTTCCACCG CTCGAAACGC TCCCTTGCGC CGCCGTGGCC GCATAACCAC 
    GTCGGAAGAT AGCATTGGAG AGTTCACGAG AGACGAAAGC AGATACGATC 
    TTAACGTTAG AGATAGAACG AGCCATTTTC GAAGTAAGTG GTTTCTTGAA 
    AAGAGAGAAA ACAGACTTTT TATTCTTG

    Gene models with high sequence identity

    1. AT4G02380.1 [+] show detail - senescence-associated gene 21
      E-value: 0; Score: 100.00

    AT4G02380.1

    Model type
    Protein coding
    Short Description
    senescence-associated gene 21
    Curator Summary
    Encodes AtLEA5 (late embryogenesis abundant like protein). Also known as SENESCENCE-ASSOCIATED GENE 21 (SAG21). Has a role on oxidative stress tolerance. mRNA levels are elevated in response to various stresses.
    Computational Description
    senescence-associated gene 21 (SAG21); CONTAINS InterPro DOMAIN/s: Late embryogenesis abundant protein, group 3 (InterPro:IPR004926); BEST Arabidopsis thaliana protein match is: Late embryogenesis abundant 3 (LEA3) family protein (TAIR:AT1G02820.1); Has 181 Blast hits to 176 proteins in 36 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 181; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT5G62690.1

    Model type
    Protein coding
    Short Description
    tubulin beta chain 2
    Curator Summary
    encodes tubulin beta-2/beta-3 chain
    Computational Description
    tubulin beta chain 2 (TUB2); FUNCTIONS IN: structural molecule activity, GTP binding, GTPase activity; INVOLVED IN: response to cadmium ion, response to salt stress, response to cold; LOCATED IN: tubulin complex, nucleolus, cell wall, plasma membrane; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Beta tubulin (InterPro:IPR002453), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Beta tubulin, autoregulation binding site (InterPro:IPR013838), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin beta chain 3 (TAIR:AT5G62700.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR