F28618

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F28618

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F28618

Determination of introduced cDNA(s)

  • RAFL04-17-P12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-P12 - Resource: pda00555

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-P12 - full reading
    TGGCACACAC TTCTCTCTCT CTCTCTCTCT GCCTCCTTTC GGATTCAAAT 
    CTCAGATCTA GCTCAACCAT GGCGTTGCTC GTCGAGAAGA CCTCAAGTGG
    CCGTGAATAC AAGGTCAAAG ACATGTCTCA AGCCGATTTC GGTCGTCTCG
    AACTCGAGCT CGCCGAAGTT GAGATGCCTG GACTCATGGC TTGTCGTACC
    GAATTCGGAC CTTCTCAGCC ATTCAAAGGC GCTAGAATCA CCGGATCTCT
    TCACATGACC ATCCAAACCG CCGTACTCAT CGAAACCCTA ACTGCTCTCG
    GTGCTGAAGT CAGATGGTGT TCCTGCAACA TCTTTTCCAC TCAAGACCAC
    GCCGCCGCAG CCATCGCTCG TGACTCCGCC GCTGTTTTCG CCTGGAAAGG
    TGAGACTCTT CAGGAGTACT GGTGGTGTAC CGAGCGTGCT CTAGATTGGG
    GTCCAGGTGG TGGTCCTGAT CTGATTGTTG ATGATGGTGG TGACGCTACT
    CTTTTGATTC ATGAGGGTGT TAAAGCTGAG GAGATCTTTG AGAAGACTGG
    TCAAGTTCCT GATCCTACTT CTACTGATAA CCCTGAGTTT CAGATCGTGT
    TGTCTATTAT CAAGGAAGGT CTTCAAGTTG ATCCTAAGAA GTACCACAAG
    ATGAAGGAGA GACTTGTTGG TGTCTCTGAG GAAACTACCA CTGGTGTTAA
    GAGGCTTTAC CAGATGCAGC AAAATGGAAC TCTTTTGTTC CCTGCCATTA
    ACGTTAACGA CTCTGTCACC AAGAGCAAGT TCGACAACTT GTATGGTTGC
    CGTCACTCAC TCCCTGATGG TCTCATGAGG GCCACTGATG TCATGATCGC
    TGGAAAGGTT GCTGTTATCT GTGGATATGG TGATGTTGGA AAGGGTTGTG
    CTGCTGCCAT GAAGACTGCT GGTGCTAGAG TCATTGTGAC TGAGATTGAT
    CCCATCTGTG CCCTTCAAGC TTTGATGGAA GGACTTCAGG TTCTTACCCT
    TGAGGATGTT GTCTCAGAAG CTGATATCTT TGTCACCACC ACCGGTAACA
    AAGACATCAT CATGGTCGAC CACATGAGGA AGATGAAGAA CAACGCTATT
    GTGTGCAACA TTGGTCACTT TGACAATGAG ATTGACATGC TTGGACTTGA
    GACTTACCCT GGTGTGAAGC GTATCACCAT CAAGCCACAG ACTGACAGGT
    GGGTGTTCCC AGAGACCAAG GCTGGAATCA TTGTCTTGGC TGAGGGTCGT
    CTGATGAACT TGGGTTGTGC CACTGGTCAC CCAAGTTTCG TGATGTCTTG
    CTCTTTCACC AACCAGGTGA TTGCCCAGCT CGAGCTCTGG AACGAGAAAG
    CAAGCGGAAA GTACGAGAAG AAGGTGTACG TTCTTCCCAA GCATTTGGAT
    GAGAAGGTTG CATTACTTCA CTTGGGCAAG CTTGGAGCCA GGCTTACAAA
    GCTGTCAAAG GACCAATCTG ACTACGTCAG CATTCCAATT GAGGGACCAT
    ACAAGCCTCC TCACTACAGG TACTGAGAAG AGAGAGAGAG TCGACAAAGC
    GGTTCAGGTT CGGATCTACT TGTGGTTTTG TGTTGGGTTG TGGTGGGAGA
    GTGGAACAGT TTGAGATATT GGTCTTCTGA TGAAGTTGAC CAAATATCAG
    TATTAATAAG GGTTATTGGC TTTTGAAGGT TGTGCTTGTT TTCTCCATTT
    TTCATGAAAC TTAAATTAGT TTTTGGTAT

    Gene models with high sequence identity

    1. AT4G13940.1 [+] show detail - S-adenosyl-L-homocysteine hydrolase
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPIR S-adenosyl-L-homocysteine hydrolase 2.1e-252
    HMMPanther S-adenosyl-L-homocysteine hydrolase 0
    PatternScan S-adenosyl-L-homocysteine hydrolase, conserved site 0
    PatternScan S-adenosyl-L-homocysteine hydrolase, conserved site 0
    HMMPfam S-adenosyl-L-homocysteine hydrolase 2.9e-143
    HMMPfam S-adenosyl-L-homocysteine hydrolase, NAD binding 7.8e-84
    Gene3D no description 7.3e-134
    superfamily Formate/glycerate dehydrogenase catalytic dom 7.1e-114
    superfamily NAD(P)-binding domain 7.8e-45
    HMMTigr S-adenosyl-L-homocysteine hydrolase 8.6e-238

    See the detailed result >

    AT4G13940.1

    Model type
    Protein coding
    Short Description
    S-adenosyl-L-homocysteine hydrolase
    Curator Summary
    Encodes a S-adenosyl-L-homocysteine hydrolase required for DNA methylation-dependent gene silencing.
    Computational Description
    MATERNAL EFFECT EMBRYO ARREST 58 (MEE58); FUNCTIONS IN: adenosylhomocysteinase activity, copper ion binding; INVOLVED IN: methylation-dependent chromatin silencing, one-carbon metabolic process, posttranscriptional gene silencing, embryo development ending in seed dormancy; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 36 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: S-adenosyl-L-homocysteine hydrolase, conserved site (InterPro:IPR020082), S-adenosyl-L-homocysteine hydrolase (InterPro:IPR000043), S-adenosyl-L-homocysteine hydrolase, NAD binding (InterPro:IPR015878); BEST Arabidopsis thaliana protein match is: S-adenosyl-l-homocysteine (SAH) hydrolase 2 (TAIR:AT3G23810.1); Has 7964 Blast hits to 7958 proteins in 1477 species: Archae - 229; Bacteria - 2334; Metazoa - 639; Fungi - 145; Plants - 247; Viruses - 0; Other Eukaryotes - 4370 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR