F30131

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F30131

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F30131

Determination of introduced cDNA(s)

RAFL05-02-J02 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-02-J02 - Resource: pda07230

full reading [+] show sequence

GTGACTTTGA AGACTCACTC TTGTTCATTC GTCTTCTCCA CCATGGAAGG 
TGTGGCTATG AAACTCCCGT CGTTCCCTAA CGCCATCTCC ATCGGCCACC 
GGAGCTTCTC CCGCGTTCGT TGCTCCTCTT CGCTATCCGT TTGTTCCGCC 
GCCGCCGCTT CCTCCGCCAC CATCTCCACT GATTCCGAAT CCGTCTCTCT 
CGGCCATCGC GTTCGAAAAG ATTTCCGAAT TCTCCACCAG GAAGTGAATG 
GGTCAAAGCT TGTGTACTTA GACAGTGCTG CAACTTCTCA GAAGCCAGCA 
GCTGTATTGG ACGCTTTGCA GAATTACTAT GAGTTCTATA ATTCCAACGT 
TCATCGAGGA ATACACTATT TAAGTGCTAA AGCTACAGAT GAATTTGAGT 
TAGCTAGGAA AAAAGTTGCT CGTTTCATCA ATGCTTCTGA TAGTAGAGAG 
ATTGTTTTTA CAAGGAATGC CACTGAAGCC ATCAATCTTG TAGCTTATTC 
ATGGGGTCTT TCTAATCTTA AACCAGGAGA CGAGGTTATA CTTACAGTTG 
CTGAACATCA TAGTTGCATT GTTCCTTGGC AAATTGTATC TCAAAAAACT 
GGAGCAGTTC TAAAGTTTGT GACTTTGAAT GAGGATGAAG TCCCTGACAT 
AAACAAGTTG AGAGAATTGA TTTCTCCAAA GACAAAGCTC GTCGCTGTTC 
ATCATGTTTC TAATGTTCTT GCATCTTCTC TTCCTATTGA AGAGATTGTG 
GTATGGGCAC ACGATGTTGG AGCGAAAGTT CTAGTGGATG CTTGTCAAAG 
TGTTCCTCAC ATGGTGGTTG ATGTCCAGAA GCTTAATGCT GATTTTCTAG 
TTGCGTCTTC TCACAAGATG TGTGGACCTA CAGGCATTGG GTTCTTATAT 
GGTAAGAGTG ATCTTCTACA TTCCATGCCT CCATTCTTAG GTGGCGGAGA 
AATGATCTCA GATGTATTTC TTGACCATTC AACTTATGCG GAACCTCCAT 
CCAGATTTGA AGCTGGAACA CCTGCAATAG GAGAAGCAAT TGCCCTTGGA 
GCAGCAGTTG ATTACTTGTC AGGAATTGGC ATGCCGAAAA TCCATGAATA 
TGAGGTAGAG ATTGGTAAGT ATTTGTACGA GAAACTGTCT TCCCTGCCTG 
ATGTGCGGAT ATACGGTCCC AGACCTTCTG AAAGTGTTCA CAGGGGTGCT 
CTTTGTTCCT TCAATGTGGA GGGCTTGCAC CCAACTGATT TGGCAACCTT 
TCTTGATCAG CAGCACGGAG TTGCAATAAG GTCAGGACAC CACTGCGCAC 
AGCCACTCCA CCGGTATCTT GGAGTGAATG CAAGTGCACG CGCCAGCCTC 
TACTTTTACA ACACAAAGGA TGACGTTGAC GCCTTCATCG TTGCGCTGGC 
AGACACTGTG AGCTTCTTCA ACTCTTTCAA ATAAGTGGAA CACCTTCCTG 
CTGGATCTCT CGGCTGGTTT GTATGGTATA TTGCTTCAGA GGCTACTTAT 
TTAAATCTGC TTTATATGAT CAATGGGAGT GGATTTTGTT TCTTCAAGTC 
CGAAGGGCAA TTTGTGTTGT AGTTAGCAAT TTGTATGAAA AAATAGACCG 
ACAGTAGTCA TGAACAATTT CAGGCCTCTA CTTGATCATA TTTGTACGAA 
TACACACGAA AGCAAATGCT TCGTTGTTAA TTTGTAAGTT TCATAAAAAA 
CAAACTCAAC ATGAAGAAAA GTTACTAATA TGAATCATCA AAGGAAAGGT 
TTCGAC

Gene models with high sequence identity

AT1G08490.1 [+] show detail - chloroplastic NIFS-like cysteine desulfurase
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Cysteine desulfurase, SufS	5.8e-263
HMMPfam	Aminotransferase, class V/Cysteine desulfurase	2.9e-144
superfamily	Pyridoxal phosphate-dependent transferase, major domain	9.8e-117
HMMPanther	CYSTEINE DESULFURYLASE	1.4e-114
Gene3D	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	7e-88

See the detailed result >

AT1G08490.1

Model type: Protein coding
Short Description: chloroplastic NIFS-like cysteine desulfurase
Curator Summary: Chloroplastic NifS-like protein that can catalyze the conversion of cysteine into alanine and elemental sulfur (S(0)) and of selenocysteine into alanine and elemental Se (Se(0)). Overexpression enhances selenium tolerance and accumulation.
Computational Description: chloroplastic NIFS-like cysteine desulfurase (CPNIFS); FUNCTIONS IN: transaminase activity, selenocysteine lyase activity, cysteine desulfurase activity; INVOLVED IN: iron incorporation into metallo-sulfur cluster, response to selenium ion, sulfur metabolic process, selenium metabolic process; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Cysteine desulfurase, SufS (InterPro:IPR010970), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: nitrogen fixation S (NIFS)-like 1 (TAIR:AT5G65720.1); Has 22660 Blast hits to 22653 proteins in 2897 species: Archae - 350; Bacteria - 15408; Metazoa - 379; Fungi - 404; Plants - 268; Viruses - 1; Other Eukaryotes - 5850 (source: NCBI BLink).
Link: InterPro Scan - TAIR