GT134

CSHL Arabidopsis genetrap mutant lines

Original: GT134

Phenotypes

Morphological phenotypes

Original description

  • stunted plants with wrinkled, shriveled rosette leaves
Anatomical entity
/structure development stage		 Phenotypes
whole plant (PO:0000003)
  • wrinkled (PATO:0001810)
sporophyte vegetative stage (PO:0007134)
  • abnormal (PATO:0000460)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)

Original: GT134

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 16542318 [Map]; Strand: w
      • AT4G34650.1 - [+] show detail - squalene synthase 2
        Distance: -59; Region: promoter
    Flanking Genome Sequence: GT134 - 3-prime edge 
    GANTANGAGT NANGACGTTT TCATCCCTAC CGACAATGGA GGATTTGTTG 
    GGGAACAAAA TGGTAGGCCA TGTGCTATAA TTCAACAAAT ATGGCCCTAT 
    TAATAAATCA ATAGACCCCA CGGCCTTTGC ATACCTTGCA CTTCCACAGA 
    ATCGCCATTA GCCTTGCTCT TAAGCTTGCC ACGGTGATAC ATGTTACTGG 
    TCCAACTATG TTCTATTTTT GCATTTAAAT TGCTTGCCGC GTGCTACACA 
    CTTAACGTTA AAAAGATAAA TCTAATCTTG ATTCATGTTT TTGTTTTATA 
    TACTTTGAAG AGACTTTTTT GCCGACCTAA AAAGAAAGAA GAGATTTCAG 
    TCTTCGATTG TCATTTTTCT TTCTTTCTTA TTGCATTTGG ATTTTATCTG 
    GTTGTTGTTG GTAAAATTAA TCTTAAAACA TGCCAAAAGA GAACGAAAGA 
    AGAAACGCAA CTAAACAAAG AAAGAACTTT AAACACAAGG AATAAAAATA 
    GATGAACAAA TGCACGTTTA GACAACGAAG GTTGTAACTA AAATAGAATC 
    CATGGTTTTA GACTAAACCA TGCTTAAACT CTGTTTGTTC TGGAAACAAT 
    CTTGCTTAGG ATTCAGTGGC GTTGAATAAA AATCAACCTA NATTACTTCC 
    TCCTCCAATT TTGNCCTNAA AATCTTGAAT GANGGTGATA CCATCAGACT 
    CNCTTACATG GATCCTCNNT TTGCTCTGAN ATATGCNAAA ACNATNGGCA 
    GTTAAAATCC CCACCATTAT TATCCTGCCT TTTCCGGGTT GAATATNTTN 
    AATATTNG

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 16542385 [Map]; Strand: w
      • AT4G34650.1 - [+] show detail - squalene synthase 2
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT134 - 5-prime edge 
    TNNNCCNNTN TCGGCTTGTC ATNGTTTCAA ACGAGTTCTG TTTTTTCTCC 
    TCTGTTTCTA AAAATGGGAA GCTTGAGTAC GATTTTGAGA CATCCGGATG 
    AGTTATATCC GCTCTTGAAG TTGAAACTTG CGATTACGAA AGCTCAGAAG 
    CAGATCCCAC TTGAGCCACA CTTAGCTTTC TGTTATTCAA TTCTTCACAA 
    AGTTTCTAAA AGCTTTTCTC TTGTTATTCA ACAACTTGGC ACCGAGCTTC 
    GTAACGCCGT ATGTTTTTTT TTCCTTNCTA NATATANCTA NNTTACATTT 
    TNANTTACAG CAATNTTTCA AACATTTCCT TGGTTTTAAT NAGGTGTNTN 
    TNTNCTACTN GATNCNCCNA NCNCTNGATA CNGTNGGTTA GNGCTNCATA 
    ACTATANTAN CCATATNTTA CCTTNGATTN GATNATTCTN CNNCNCNCNC 
    NAANAAA

AT4G34650.1

Model type
Protein coding
Short Description
squalene synthase 2
Curator Summary
Encodes a protein with similarity to squalene synthase which catalyzes the first committed step in sterol biosynthesis. To date no experimental evidence exists that SQS2 functions as a squalene synthase and some experiments indicate it does not have this function.
Computational Description
squalene synthase 2 (SQS2); FUNCTIONS IN: farnesyl-diphosphate farnesyltransferase activity; INVOLVED IN: N-terminal protein myristoylation, sterol biosynthetic process; LOCATED IN: integral to membrane; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Terpenoid synthase (InterPro:IPR008949), Farnesyl-diphosphate farnesyltransferase (InterPro:IPR006449), Squalene/phytoene synthase (InterPro:IPR002060); BEST Arabidopsis thaliana protein match is: squalene synthase 1 (TAIR:AT4G34640.1); Has 1176 Blast hits to 1175 proteins in 496 species: Archae - 34; Bacteria - 579; Metazoa - 114; Fungi - 168; Plants - 163; Viruses - 0; Other Eukaryotes - 118 (source: NCBI BLink).
Link
InterPro Scan - TAIR