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GT14160

CSHL Arabidopsis genetrap mutant lines

Original: GT14160

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT14160

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

  • 3 prime edge [+] show sequence

    Not mapped

    Flanking Genome Sequence: GT14160 - 3-prime edge 
    GGACATCCGG GAGGAGCTTT CTCCGTCTT

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 2939281 [Map]; Strand: w
      • AT1G09100.1 - [+] show detail - 26S proteasome AAA-ATPase subunit RPT5B
        Distance: 0; Region: exon1 (5UTR)
      • AT1G09110.1 - [+] show detail - pre-tRNA
        Distance: -136; Region: promoter
    Flanking Genome Sequence: GT14160 - 3-prime edge 
    GTAGAGGTAT TTTACCGACC GTTACCGACC GTTTTCATCC CTACTTACAG 
    TGATTAACTC AGGCTGCTAA GGGAGATGGC AACGGCGATG GCGGAGGATA 
    CGAGCTTCGA GGGAGACCAA CTAGCTTCCA TGACTACTGA TGACATCGGT 
    AGAGCTTCTC GTCTCTTAGC CAACGAGATT CGCATCCTCA AGGTCACCAC 
    CCTCTCTCTC TTCTCTCTAT ATTTTTAATC TGCTGGCTAC TTGGAACTAG 
    TATATTTCTT CATTCCAAGC TCACTATTAC CTTAATTTGA TTAGCGACAC 
    TTTGATTAGT TTTGAAGAAG ATGGATGATG TCTGAGAGCA ACGATAACTC 
    TTGTTT

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 18250486 [Map]; Strand: w
      • AT3G49220.1 - [+] show detail - Plant invertase/pectin methylesterase inhibitor superfamily
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT14160 - 5-prime edge 
    CCTACTTTCT CCATGACCGT GTACTGAAGG ATTCGACGGC GTCGACGACG 
    GTGGAGTGAA GGATCATATG ACGGCGGCGT TACAGAATCT TTCTGAGCTT 
    GTTAGTAACT GTCTTGCTAT ATTCTCGGCG AATCACGACG GTGACGATTT 
    CGCCGGAGTT CCAATACAGA ACAGGAGGCT GCTGGGAGTG GAGGAACGAG 
    AAGAGAAATT CCCGAGATGG ATGAGACCAA AAGAACGCGA GATACTGGAA 
    ATGCCGGTAT CTCAGATACA AGCTGATATT ATCGTATCGA AAGACGGTAA 
    CGGCACGTGC AAAACTATAT CGGAAGCTAT CAAGAAAGCT CCTCAGAACA 
    GTACTCGCCG GATAATTATC TACGTCAAGG CCGGAAGGTA AAATGACTGT 
    TTTACCCTTC GTATTAATCG ATGTTTTTAA CTGTTATGTC CGTGAATGAC 
    GAAAGTGAAA ACTAAAAACC TGTACGGAGG TTTAATAATT GCGTATCTCT 
    GACTGTGTCT GTGATCTCTG TTTTTTTTTT T

AT1G09100.1

Model type
Protein coding
Short Description
26S proteasome AAA-ATPase subunit RPT5B
Curator Summary
Encodes RPT5b (Regulatory Particle 5b), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.
Computational Description
26S proteasome AAA-ATPase subunit RPT5B (RPT5B); FUNCTIONS IN: ATPase activity, calmodulin binding; INVOLVED IN: glucose mediated signaling pathway, proteasomal protein catabolic process, embryo sac development, pollen development; LOCATED IN: proteasome complex, plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-type, conserved site (InterPro:IPR003960), 26S proteasome subunit P45 (InterPro:IPR005937); BEST Arabidopsis thaliana protein match is: regulatory particle triple-A ATPase 5A (TAIR:AT3G05530.1); Has 33470 Blast hits to 31156 proteins in 3185 species: Archae - 1434; Bacteria - 12401; Metazoa - 4942; Fungi - 3663; Plants - 3251; Viruses - 53; Other Eukaryotes - 7726 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G09110.1

Model type
Pre-tRNA
Short Description
pre-tRNA
Computational Description
tRNA-Glu (anticodon: TTC)
Link
InterPro Scan - TAIR

AT3G49220.1

Model type
Protein coding
Short Description
Plant invertase/pectin methylesterase inhibitor superfamily
Computational Description
Plant invertase/pectin methylesterase inhibitor superfamily; FUNCTIONS IN: enzyme inhibitor activity, pectinesterase activity; INVOLVED IN: cell wall modification; LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pectinesterase, active site (InterPro:IPR018040), Pectin lyase fold/virulence factor (InterPro:IPR011050), Pectinesterase inhibitor (InterPro:IPR006501), Pectinesterase, catalytic (InterPro:IPR000070), Pectin lyase fold (InterPro:IPR012334); BEST Arabidopsis thaliana protein match is: pectin methylesterase PCR fragment F (TAIR:AT5G53370.1); Has 3279 Blast hits to 3212 proteins in 470 species: Archae - 6; Bacteria - 866; Metazoa - 1; Fungi - 193; Plants - 2188; Viruses - 0; Other Eukaryotes - 25 (source: NCBI BLink).
Link
InterPro Scan - TAIR