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GT15169

CSHL Arabidopsis genetrap mutant lines

Original: GT15169

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT15169

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25098197 [Map]; Strand: w
      • AT5G62520.1 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
      • AT5G62520.2 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT15169 - 3-prime edge
    AACGGTAGAG GATTTTACCG ACCGTTACCG ACCGTTTTCA TCCCTAACTC 
    CATAAGCCCA AGCTCGTCAG CAAAAGATCG ATCAGAAGAA GAATCGCAGC
    TTCCAGATTC AGAGATGGTT GATCCATCTT GCTCAGAATC ATCAAAAACA
    GCTTCGACTT GGGTTCTCAC GTAATCCATT GAAAAATAGG AACTTTAGAA
    CCGAATGAGA AAAGAAACTT TATAAAGCTT TTGAATTAGT ATTTTGTTAC
    TTGAGAGAGG AAAAGAACTT GAGGTCCAAG TTCATATTTA TAGCTATGAC
    TTAAGGAAAT AATTACACAT ATGGAAAAAA GAACCGCCTT TTGTTTCTGT
    TACCGTCGGT TTTGCAATTT TTATAAATAT CAAAAACTCA GAGTTTCTTG
    TCTTTTTCTC CGTGGAGAAG ACGTAAACAA ACATTTAACA GCTAAATATC
    TTTCTTATTT TATTACATAA TAAATACTTT GGTTAAGTTT TTGTAAGTTG
    CGGGCGCAAG CTGTTTTTTT TTCTTTGAGG GGTCTATTCC GTGTTGGAGT
    TCGTTAAGCG
  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25098251 [Map]; Strand: w
      • AT5G62520.1 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
      • AT5G62520.2 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT15169 - 5-prime edge
    TCCTACTTTC TCCCTGTATG GAGTTGCTTG AAGGAGATAA AGCTCATGAT 
    TTGATCTTTC GTAATTGTAA ATCTGGTCTT GGTGATCAAT GTCAGATTCT
    CTCTGTTCTT AGAAACGGAT TCAGGAACGT TGGATCTCGT GCAAAGCTCA
    AGACTTTCCA GGTTTTTCAA GAGGCGGTGC AGATGAAACA CGGTGGACAC
    GGCGGAGCTA AGGTGAAATA CGGTTGGTGC TCTGTTTCGA AACATGAGCT
    TAAAACGATT TTTGAGTATG GGTTTAGCGA GCCGCTGAGA AATGATGGAT
    CTTTTGGCCG TGGATTGTAT CTCTCGCCGG ATAATTCTCC TCTTGATTGG
    TAAGTTTCTC TAAATCTCTA AAAGTTTCAA TTTTTCGAAA TTCATTGGTC
    TCAAAATCTC ATTTTTGGTG TTTGTTTGTT TGATTAGTTT GAAGGATTCA
    GCTTCAGAAT CAGAAGATGG GATGAGATTC TTGTTGCTTT GTAGAGTTCT
    ACTTGGAAAA TCAGAGATTG TTCCTCAAGG CTCGACTAGA TCATGTCCAA
    GTTCACCAGA GTTTGATTCT GGTGTGGATG ATTTAGTTTC TACGAAGAAG
    TATATTGTGT GGAGTACACA CATGAACACT CATGTTTTGC CTGAGTTTCT
    TGTTTGCATC AAAGCTCCAT TTAACCTAAC TCGTAAGC
  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25098256 [Map]; Strand: w
      • AT5G62520.1 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
      • AT5G62520.2 - [+] show detail - similar to RCD one 5
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT15169 - 5-prime edge
    CTTTCTCCCT GTATGGAGTT GCTTGAAGGA GAAAAGCTCA TGATTTGATC 
    TTTCGTAATT GTAAATCTGG TCTTGGTGAT CAATGTCAGA TTCTCTCTGT
    TCTTAGAAAC GGATTCAGGA ACGTTGGATC TCGTGCAAAG CTCAAGACTT
    TCCAGGTTTT TCAAGAGGCG GTGCAGATGA AACACGGTGG ACACGGCGGA
    GCTAAGGTGA AATACGGTTG GTGCTCTGTT TCGAAACATG AGCTTAAAAC
    GATTTTTGAG TATGGGTTTA GCGAGCCGCT GAGAAATGAT GGATCTTTTG
    GCCGTGGATT GTATCTCTCG CCGGATAATT CTCCTCTTGA TTGGTAAGTT
    TCTCTAAATC TCTAAAAGTT TCAATTTTTC GAAATTCATT GGTCTCAAAA
    TCTCATTTTT GGTGTTTGTT TGTTTGATTA GTTTGAAGGA TTCAGCTTCA
    GAATCAGAAG ATGGGATGAG ATTCTTGTTG CTTTGTAGAG TTCTACTTGG
    AAAATCAGAG ATTGTTCCTC AAGGCTCGAC TAGATCATGT CCAAGTTCAC
    CAGAGTTTGA TTCTGGTGTG GATGATTTAG TTTCTACGAA GAAGTATATT
    GTGTGGAGTA CACACATGAA CACTCATGTT TTGCCTGAGT TTCTTGTTTG
    CATCAAAGCT CCATTTAACC TAACTCGTAA GCAAAATCAA AATCTCTAAT
    CTTGAGTTTT TC

AT5G62520.1

Model type
Protein coding
Short Description
similar to RCD one 5
Curator Summary
Encodes a protein with similarity to RCD1 but without the WWE domain. The protein does have a PARP signature upstream of the C-terminal protein interaction domain. The PARP signature may bind NAD+ and attach the ADP-ribose-moiety from NAD+ to the target molecule. Its presence suggests a role for the protein in ADP ribosylation. Up-regulated by NaCl. SRO5 and P5CDH (an overlapping gene in the antisense orientation) generate 24-nt and 21-nt siRNAs, which together are components of a regulatory loop controlling reactive oxygen species (ROS) production and stress response.
Computational Description
similar to RCD one 5 (SRO5); FUNCTIONS IN: NAD+ ADP-ribosyltransferase activity; INVOLVED IN: oxygen and reactive oxygen species metabolic process, response to salt stress; LOCATED IN: mitochondrion; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Poly(ADP-ribose) polymerase, catalytic domain (InterPro:IPR012317), RST domain of plant C-terminal (InterPro:IPR022003); BEST Arabidopsis thaliana protein match is: similar to RCD one 4 (TAIR:AT3G47720.1); Has 162 Blast hits to 153 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 159; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G62520.2

Model type
Protein coding
Short Description
similar to RCD one 5
Curator Summary
Encodes a protein with similarity to RCD1 but without the WWE domain. The protein does have a PARP signature upstream of the C-terminal protein interaction domain. The PARP signature may bind NAD+ and attach the ADP-ribose-moiety from NAD+ to the target molecule. Its presence suggests a role for the protein in ADP ribosylation. Up-regulated by NaCl. SRO5 and P5CDH (an overlapping gene in the antisense orientation) generate 24-nt and 21-nt siRNAs, which together are components of a regulatory loop controlling reactive oxygen species (ROS) production and stress response.
Computational Description
similar to RCD one 5 (SRO5); FUNCTIONS IN: NAD+ ADP-ribosyltransferase activity; INVOLVED IN: oxygen and reactive oxygen species metabolic process, response to salt stress; LOCATED IN: mitochondrion; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Poly(ADP-ribose) polymerase, catalytic domain (InterPro:IPR012317); BEST Arabidopsis thaliana protein match is: similar to RCD one 4 (TAIR:AT3G47720.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR