GT9389

CSHL Arabidopsis genetrap mutant lines

Original: GT9389

Phenotypes

Morphological phenotypes

Original description

  • semi-sterile
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT9389

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 1585436 [Map]; Strand: w
      • AT4G03560.1 - [+] show detail - two-pore channel 1
        Distance: 0; Region: intron21 (CDS)
    Flanking Genome Sequence: GT9389 - 5-prime edge 
    TTTCTCTCTG TCCATAAAGT TACAGATGTT GGGGAGATTA GAAAAGTAAA 
    GAACCTTTTG GTTCGTGTTC AATCGGCAAT GTTTTCATCT CGCTCTAGTA 
    TCGCTGCAGG ATTCTCAAGA AAAGAGAAAC AGGCGTCGAT CTGCATGGTA 
    AGTAGATAGA TCAAATTGTT GGTTGGTAAT ATCATACTAG ACTCACTGAT 
    CTGAAGGTTT ATTTATGTTT TTGTGGTTAT TAAAGGTCGA ATTCTCAGAG 
    TCAAAGAGTT GATACACTTC TTCATCACAT GTTGGGGGAT GAACTGAGCA 
    AACCAGACTG TTCCACTTCT GACACATAAA ACAAAACAAG ATCGTTAAGA 
    GCTTATTTAT ACGTTTATAC TGTGAATCTT TGATTCTATA TGGAGAGATC 
    AAATAGAATC TAGAAGGTGA CTACATTTTT TCTTCTTTTG GGTGAAGATT 
    TAATATAAAA CGATTTGTGT TATAGTATGA TCTTTTTGGC TTTTCATTTG 
    CTTAGTTAGT AGTGTTTTGT AATGAA

AT4G03560.1

Model type
Protein coding
Short Description
two-pore channel 1
Curator Summary
Encodes a depolarization-activated Ca(2+) channel. Anti-sense experiments with this gene as well as Sucrose-H(+) symporters and complementation of yeast sucrose uptake mutant cch1 suggest that this protein mediates a voltage-activated Ca(2+ )influx. Mutants lack detectable SV channel activity suggesting TPC1 is essential component of the SV channel. Patch clamp analysis of loss of function mutation indicates TPC1 does not affect Ca2+ signaling in response to abiotic and biotic stress.
Computational Description
two-pore channel 1 (TPC1); FUNCTIONS IN: voltage-gated calcium channel activity, calcium channel activity; INVOLVED IN: regulation of jasmonic acid biosynthetic process, calcium ion transport, calcium-mediated signaling, seed germination, regulation of stomatal movement; LOCATED IN: vacuolar membrane, plasma membrane, vacuole, plant-type vacuole; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: EF-HAND 2 (InterPro:IPR018249), Ion transport (InterPro:IPR005821), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR