K00843

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K00843

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K00843

Determination of introduced cDNA(s)

  • J023114O06 - Rice Full-length cDNAs - Original: J023114O06

    full reading [+] show sequence

    Nucleotide sequence: J023114O06 - full reading 
    GACTGCTGCA CTCGCGGAGG CGACCAGGAT TGCTCGTCGT CCTCCACGGA 
    GATGGCGGCA TTGCCGGCCT ATCTCCTCCT CCTCCTCCTC ATCCTGGTGA 
    TCCTCCGACC AGCGGCTGCG CCCGGCGCCG CTTCGCAGCC GCCTACCTCG 
    GAGCCTCACC TCCCGCCGCC GCTGCTTGCT CAGAAGGTGC AGGTGTGGCC 
    CAAGCCGACG TCCATCTCGT GGCCGTCCGC GGTGTACGCG CCGCTCTCGC 
    CGTCGTTCAG CGTCCGCGCG GTGCTGTCCC ACCCGTCGCT CCGCCAGGCG 
    GTAGCCTTCT ACACCCGCCT CATCCGCGCC GAGCGCCACG CGCCGCTGGT 
    TCCCCCCGCC AACTACACCC TCTCCCGCGT CCCCGTCCGC ACCCTCACGC 
    TCTCCGTCTC CGACCCCGAC GTCCCGCTCG GCCCCGCCGT CGACGAGTCC 
    TACACGCTCT CCGTGCTCCC GGACTCCGGA TCGGCCGATA TCTCCGCCGC 
    CACGCCATGG GGCGCCATCC GCGGGCTCGA GACCTTCTCC CAGCTCGCCT 
    GGGCCGGCGG CGGCGCCGCC TCCGGTGGCC AGCCGATCGT CCCCAGCGGC 
    ATCGAGATCT CCGACCGCCC ACACTTCACC CACCGCGGCA TCCTCCTCGA 
    TACCGCCAGG AATTTCTACC CGGTCCGCGA CATCCTCCAC ACCCTCCGCG 
    CCATGGCCTT CAACAAGCTC AATGTCTTCC ACTGGCACAT CACCGACGCC 
    CAGTCCTTCC CCATCGTCCT CCCTACCGTC CCGAACCTCG CCAACTCCGG 
    CTCCTACTCC CCCACCATGC GCTACACCGA GAACGACGTC CGGCACATCG 
    TCAGCTTCGC CGCCTCCTTC GGCATCCGCG TCATCCCCGA GATCGACATG 
    CCCGGGCACA CGGGTTCGTG GGCGGGAGCG TACCCGGAGA TCGTCACCTG 
    CGCGAACAGG TTCTGGGCGC CGCACGCGGA GCCGGCGCTG GCGGCGGAGC 
    CCGGCACGGG GCAGCTGAAC CCGCTCAACC CCAAGACGTA CCGCGTGGCG 
    CAGGACGTGC TGCGCGACAT GGTGGCCCTG TTCCCGGACC CGTACCTCCA 
    CGGCGGCGCC GACGAGGTGA ACACGGCGTG CTGGGAGGAC GACCCGGTGG 
    TGCGCCGGTT CCTCGCCGAG GGCGGCACGC ACGACCACCT CCTGGAGCTG 
    TTCATCAACG CGACGCGCCC GTTCGTGGCG CAGGAGCTGA ACCGCACGGT 
    GGTCTACTGG GAGGACGTCC TCCTCGGGCC CAAGGTCACC GTCGGCCCCA 
    CGATTCTGCC CAGGGAGACC ACCATACTGC AGACGTGGAA CGACGGGCCG 
    GAGAACACGA AGCGCGTCGT CGCCGCGGGC TACCGCGCCA TCGTGTCGTC 
    GGCGTCGTAC TACTACCTGG ACTGCGGCCA CGGCGGGTGG GTGGGCAACG 
    ACAGCCGGTA CGACAAGCAG GAGAAGGAGC GCGAGGGCAC GCCGCTGTTC 
    AACGACCCCG GCGGCACGGG CGGGTCATGG TGCGCGCCGT TCAAGACGTG 
    GCAGCGCGTC TACGACTACG ACATCCTGCA CGGGCTGACC GACGACGAGG 
    CGCAGCTGGT GCTGGGCGGG GAGGTGGCGC TGTGGTCGGA GCAGTCGGAC 
    GAGACGGTGC TGGACGCCAG GCTGTGGCCG AGGGCCGCCG CCGCCGCGGA 
    GACGCTCTGG TCCGGCAACA AGGGCTCCAA CGGGAAGAAG CGCTACGCGA 
    ACGCCACCGA CCGCCTCAAC GACTGGAGGC ACCGGATGGT CGAGCGCGGC 
    ATCCGCGCCG AGCCCATCCA GCCGCTCTGG TGCTCGCTCC ACCCCGGCAT 
    GTGCAACCTG TCGCAGTAGC AGTGTCAGTT TGCTCCATCG CTTCGCCTCG 
    CTGTCGCCTG CACAGTGCGA AGGAATGTTC AGAAATCCAA GTGACAGAAG 
    AAGAATCCGC ATCGATCTTG GGGGCGTGAC GTCGTACACT GATTGCTGAA 
    GAAACCAAGA ACAGAATAAA CGAATGCCTA CTAAACAAAT GTTCTGTTCT 
    GATGCCCTCT TCCGGAGGGA ACCCGCCGAT TGCGAATTGT TCAAGAAAGT 
    CGCGGACTTA TGCCGTGCAG AAAAGAAAGT TTTCTTTGTG AGTAGTTTCT 
    GCACTTGATG CTGAACAGAT TTTTGCACTG TTTATTCCTT CATTTTCACA 
    ATAACACGGG TAGATAGAGT AGTAGTAGTA TTTGGCTAGT GCAATAGATG 
    AACAAGGTTG TAGTAGAAGT GCAGAACCAT CTGGTTGTAG TTTTGTACGA 
    AGGGTATCTT AATAGAAAAA AGTTGGG

    Gene models with high sequence identity

    1. AT1G05590.1 [+] show detail - beta-hexosaminidase 2
      E-value: 2e-177; Score: 619.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Glycoside hydrolase, catalytic core 1.2e-105
    superfamily beta-N-acetylhexosaminidase-like domain 2.7e-21
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    FPrintScan Glycoside hydrolase, family 20 5.6e-55
    Gene3D no description 1.8e-16
    Gene3D Glycoside hydrolase, subgroup, catalytic core 5e-108
    HMMPanther Glycoside hydrolase, family 20 6.2e-130
    HMMPfam Glycoside hydrolase, family 20, catalytic core 2.8e-95
    HMMPfam Beta-N-acetylhexosaminidase, subunit a/b 1.3e-11

    See the detailed result >

    AT1G05590.1

    Model type
    Protein coding
    Short Description
    beta-hexosaminidase 2
    Curator Summary
    Encodes a protein with β-hexosaminidase activity (the enzyme is active with p-nitrophenyl-β-N-acetylglucosaminide as substrate but displayed only a minor activity toward p-nitrophenyl-β-N-acetylgalactosaminide). Chitotriose-PA was digested almost completely overnight by a 50% ammonium sulfate fraction of a supernatant yeast expressing AtHEX3.
    Computational Description
    beta-hexosaminidase 2 (HEXO2); FUNCTIONS IN: UDP-glucosyltransferase activity, hexosaminidase activity, beta-N-acetylhexosaminidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 20, catalytic core (InterPro:IPR015883), Beta-N-acetylhexosaminidase, subunit a/b (InterPro:IPR015882), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, family 20 (InterPro:IPR001540), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: beta-hexosaminidase 1 (TAIR:AT3G55260.1); Has 3806 Blast hits to 3725 proteins in 709 species: Archae - 2; Bacteria - 2403; Metazoa - 477; Fungi - 234; Plants - 128; Viruses - 0; Other Eukaryotes - 562 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR