K02338

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K02338

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K02338

Determination of introduced cDNA(s)

  • J013038J10 - Rice Full-length cDNAs - Original: J013038J10

    full reading [+] show sequence

    Nucleotide sequence: J013038J10 - full reading 
    GACACACCTT GGATCGCGGC CTCACGCGCG TGAGGCGCCG CAACGCAACG 
    TGCGACCCCG ACCTCTCCTC CTCCACCTCG GCTTCCCCCC AAATTCCATC 
    TCCTCGCCTC GGCGAATCCG ATTCCGAGCA CTCACAACCG GATCGGAGCG 
    AGTTCCATGG CCTGAATCTC AGGGGGGGTG ACTGGCCGAG GAGCGGGCTC 
    TCTCTCTCAC TGCCTTAGGC TGAAATCTGC TATAACTTTG GAGGTACTAA 
    AACGAGTCTG AGATGGGTGT GATTAGTACG GTGCTTGGTT TCTCTGGGTT 
    TGGCTTTGGA TTCTCCGCTG GTATTGTCAT CGGATACTAC TTCTTCATCT 
    ACTTCCAGCC AACTGATGTC AAGGACGTCA AAGTTCGCCC ACTTGTCGAA 
    TATGACTCAA TATCGTTGGA CGGTATCCTT CCTGAAATTC CTTTGTGGGT 
    CAAGAATCCT GACTATGATA GAATTGATTG GCTGAATAGA TTTTTGGAGA 
    TGATGTGGCC TTACCTCAAC AAGGCGATCT GCAGAACTGC ACTGGATATT 
    GCAAAGCCAA TCATTGAAGA GAACAGGAAG ACGTATAAAC TAGAATCTAT 
    TGAGTTTGAA TCACTTACAT TGGGTAGCTT ACCACCCACC TTTCAAGGAA 
    TGAAAGTGTA TGTCACAGAA GAACAAGAAT TGATAATGGA ACCATCTCTT 
    AAGTGGGCTG CAAATCCCAA TGTCACTGTT GTTGTAAAGG CTTATGGGTT 
    GAAAGCAACT GTCCAGATTG TGGATCTGCA AGTCTTTGCT TCACCTCGTA 
    TTACTCTGAA GCCATTGGTC CCTACATTTC CTTGCTTCGC AAAAATCCTT 
    GTTTCTCTCA TGGAGAAGCC ACACGTTGAC TTCGGGCTGA AACTTTTCGG 
    AGCAGATTTA ATGGCTATCC CTGGTCTTTA CAGATTTGTT CAGGAGACCA 
    TAAAAAAGCA AGTTGCGAGC ATGTATTTGT GGCCAAAGAC ATTGGAAGTT 
    CCCATAATGG ATCCCTCAAA AGCTTCTAAG AAGCCTGTTG GAATTCTACT 
    TGTGAAAGTT TTAAGAGCTC AAAACCTGCG AAAGAAGGAT CTGTTGGGTA 
    AATCGGACCC ATATGTGAAA CTTAAGATGT CAGATGACAA GCTTCCGTCC 
    AAGAAAACAA CAGTGAAGCG TAGCAATCTC AATCCAGAGT GGAATGAGGA 
    TTTCAAATTT GTTGTGACAG ACCCTGAAAC TCAGGCACTG GAAATTAATG 
    TCTTTGATTG GGAACAGGTT GGCAAGCATG AGAAAATGGG CATGAACAAC 
    ATTCTGTTGA AAGAACTTCC CGCAGATGAG ACTAAAGTCA TGACTGTTAA 
    CTTGCTTAAG ACGATGGATC CTAATGATGT ACAAAATGAG AAGTCCCGTG 
    GTCAGCTTAC TCTGGAGGTG ACATATAAGC CTTTCAAGGA AGAGGATATG 
    GAGAAAGAAG GCATCGATAA TGCTGATGTG GTGGAGAAAG CTCCTGATGG 
    TACTCCAGCT GGTGGAGGAC TGCTTTATGT TGTTGTTCAT GAAGCTCAAG 
    ATCTTGAAGG AAAGCATCAC ACAAACCCAT ATGCAAAGAT AATTTTCAAA 
    GGAGAAGAGA AGAAAACAAA GGTAATCAAG AAAAACAGGG ATCCACGGTG 
    GGAGGACGAA TTCGAGTTTG TATGCGAGGA ACCCCCTGTA AATGATAAAC 
    TGCATATTGA AGTTCTAAGT AAAGCTTCAA AGAAAGGATT GATACACGGC 
    AAGGAAACCT TAGGCTACAT TGATATAAGC CTCGCAGATG TGATCAGCAA 
    CAAGCGGATC AATGAGAAGT ACCACCTCAT TGATTCCAAA AATGGTCAGA 
    TTCAGATAGA GATGCAGTGG AGAACTTCAT AGCAACCGTG ATGAAGTAGG 
    TGAAATGCTT GAACTTCGTG AGATACAGGA TGATACATAT GAATGTTGTT 
    GCTACAAACA GAGTTTGGCC AATACCTGTG ATTGAGTTCT GTAGATATTT 
    ACTTCTTTGT GTCCAGGCAT CTGTTTTACC TTGTTGAGAT ATTTTGTTAT 
    TTTTTCAAAA TTGTGTTGAG AACAACGTAG AAGCAACCAT GCTATTGGCA 
    TCGGAAGTGG AATTAGATGT GAGATGTAAT GACCCATTAT CCTTC

    Gene models with high sequence identity

    1. AT2G20990.1 [+] show detail - synaptotagmin A
      E-value: 0; Score: 738.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam C2 calcium-dependent membrane targeting 4.3e-25
    HMMPfam C2 calcium-dependent membrane targeting 8.7e-14
    ProfileScan C2 membrane targeting protein 17.457
    ProfileScan C2 membrane targeting protein 11.034
    FPrintScan C2 region 3.4e-07
    FPrintScan C2 region 3.4e-07
    FPrintScan C2 region 3.4e-07
    HMMSmart C2 calcium-dependent membrane targeting 1e-22
    HMMSmart C2 calcium-dependent membrane targeting 4.3e-16
    superfamily C2 calcium/lipid-binding domain, CaLB 4.9e-33
    superfamily C2 calcium/lipid-binding domain, CaLB 4.8e-24
    HMMPanther CALCIUM LIPID BINDING PROTEIN 1.9e-196
    HMMPanther CALCIUM-DEPENDENT LIPID-BINDING PROTEIN (CALB 1.9e-196
    Gene3D no description 1.1e-27
    Gene3D no description 1.3e-21

    See the detailed result >

    AT2G20990.1

    Model type
    Protein coding
    Short Description
    synaptotagmin A
    Curator Summary
    Encodes a plasma membrane localized protein with similarity to synaptotagmins, a class of membrane trafficking proteins. SYT1 is expressed in all tissues. Loss of function mutations show hypersensitivity to NaCl and electrolyte leakage from the plasma membrane. SYT1 also affects calcium dependent freezing tolerance. SYT1 probably plays a role in membrane repair such as membrane resealing after freezing induced damage. Regulates endocytosis endosome recycling at the plasma membrane, but not membrane traffic along the secretory pathway.
    Computational Description
    synaptotagmin A (SYTA); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: in 7 processes; LOCATED IN: endosome, plasma membrane, vacuole, membrane; EXPRESSED IN: whole plant, guard cell, cultured cell, callus, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: C2 membrane targeting protein (InterPro:IPR018029), C2 calcium/lipid-binding domain, CaLB (InterPro:IPR008973), C2 region (InterPro:IPR020477), C2 calcium-dependent membrane targeting (InterPro:IPR000008); BEST Arabidopsis thaliana protein match is: Calcium-dependent lipid-binding (CaLB domain) family protein (TAIR:AT1G20080.1); Has 7068 Blast hits to 5182 proteins in 270 species: Archae - 0; Bacteria - 0; Metazoa - 4491; Fungi - 774; Plants - 1307; Viruses - 0; Other Eukaryotes - 496 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR