K04041

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K04041

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K04041

Determination of introduced cDNA(s)

J013157D07 - Rice Full-length cDNAs - Original: J013157D07

full reading [+] show sequence

GGATACCTTT CTTCTCCTTT CCCCATCGCC TCCTCACCCA GAGAGAAGAG 
GAGGTTGGAG GAGGAGGCGA AGAGGCTAGC TAGCTAGCTA GGGTTTCGTG 
CTCCCCATCG CCTCGCGCGC GCAGCCGGAT CCGATCGGGC GTCCTCGTCG 
GCGTCGGCGG CGGTGCCCCT GATATCAGTG TTGTGATTCC GTTGCAATGG 
CATCTGGAAC TGATGAGAAA GCCAAGATGG AGGGTTTGAC ATCAGCTGCA 
GCCTTTGTTG AGGGTGGGAT TCAGGATGCA TGTGATGATG CATGTAGTAT 
CTGCCTTGAG GCGTTCTGTG AGAGTGACCC TTCCACATTG ACTGGTTGCA 
AACACGAGTT CCACCTCCAA TGCATTCTTG AATGGTGTCA GAGAAGTTCT 
CAGTGTCCTA TGTGTTGGCA GCCTATCAGT TTGAAGGATC CTACCAGTCA 
AGAGCTGCTC GAGGCAGTGG AGCGTGAAAG GAATGTAAGG ACCAATCAAA 
CTCGAAATAC AACTATATTT CATCATCCTG CTCTTGGAGA TTTTGAGGTT 
CAGCATTTAC CTGTTGTTGG TAATGATGCT GAACTTGAAG AGCGTATATT 
ACAGCACCTA GCAGCAGCCG CTGCAATGGG AAGGTCACAC CACCTTGGTA 
GAAGAGAAGG ACACAGGGGT CGTTCCGGCT CTCATGGTCG TCCACAGTTC 
TTAGTTTTTT CTTCGCATCC AAACATGCCT TCTGCTGGTT CAGTTTCTTC 
ATCGTCCGTT CAAGGGGAAG TGGATAATGA ATCAAGTCCT GTCCACACAA 
CTGGTGAATT ATCACTGCAT GCTAACACCC ATGAAGAAGC AGGCAATCAA 
AGTCCTGGGA TGCTTACCTA CGATGCTGAT CAAGATGCTG TTGTTTCATC 
TGGAAATAGT ACCCCTGTAT CTAGCCCTAG GTTTTTCAAC AGGAGGCATT 
CCACTGGGCA ATCAACTCCA GTAAACAACG ACAGAGCTGG GCCTTCAGAT 
CTTCAGTCTT TCTCAGACTC TCTGAAGTCT CGCTTAAATG CTGTCTCTAT 
GAAGTACAAG GAATCTATTA CAAAAAGTAC TCGAGGATGG AAGGAGAGAC 
TTTTTTCGCG TCATTCATCT GTGGCAGATC TTGGTTCTGA AGTAAGAAGA 
GAAGTTAATG CTGGAATTGC ATCCGTATCA AGGATGATGG GGCGTCTGGA 
AACTAGAGGT AGTAATGGTA GAACAAGTGA TGGCCCAGCA ATATCCACTT 
CTGAAGTTAT TCCCAGTACA GAATCAAGCA ATGAGAGAGT TACAGAAAAC 
AATCCAACTA CTGCAGCGAC AGGTAGTGGC AACACTTCTG CGTCTTCTGC 
CCCTTGTGTT ACAACAACCG GTTCAAATTA GCATGTTATA GGTTCCGAAA 
GAAGGCCTGC ACATTCCCAT TCTTCTCCAT CAAAGGATTG CCATTGCCAG 
GCATGGGCAG GTGACCAAAA GCAACGTCAT GTTGTCATCC TCAGCATTGC 
ATAAATCACC TCCAGAAAAT CGGAATGACA AATTTGATGT ATTGGTTTGA 
TAAAAGGGCT CAGCCCGAGG TATTACCTTA GGATGCCGAT TTGTTCTGCT 
TCATCTATGT AAAAAGGGAA AAAAATCAGG GACAAGTTTT TACCGGTATA 
AATAATGGAT TTGGTGACAT GATAGCAATC ATAATCGTG

Gene models with high sequence identity

AT5G22000.3 [+] show detail - RING-H2 group F2A
E-value: 4e-98; Score: 355.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	RING FINGER PROTEIN 11 (SID 1669) (NEDD4 WW D	2.6e-11
Coil	coiled-coil	0
superfamily	RING/U-box	4.6e-14
HMMPfam	Zinc finger, C3HC4 RING-type	1.4e-06
Gene3D	no description	5.9e-08
HMMSmart	Zinc finger, RING-type	9.3e-06
ProfileScan	Zinc finger, RING-type	12.032

See the detailed result >

AT5G22000.3

Model type: Protein coding
Short Description: RING-H2 group F2A
Curator Summary: encodes a RING-type E3 ubiquitin ligase implicated in gametogenesis. Double mutant analyses with RHF1a suggests that RHF2a may be involved in targetting ICK4KRP6 for degradation following meiosis in order to allow the mitoses associated with megagametogenesis and microgametogenesis to occur. RHF2a is expressed in all four floral whorls and is present at ~8-fold higher levels than RHF1a in inflorescences by RT-PCR analyses.
Computational Description: RING-H2 group F2A (RHF2A); FUNCTIONS IN: zinc ion binding; INVOLVED IN: regulation of cell cycle, proteolysis involved in cellular protein catabolic process, megagametogenesis, microgametogenesis; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957); BEST Arabidopsis thaliana protein match is: RING-H2 group F1A (TAIR:AT4G14220.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR