K08146

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K08146

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K08146

Determination of introduced cDNA(s)

  • J013024H13 - Rice Full-length cDNAs - Original: J013024H13

    full reading [+] show sequence

    Nucleotide sequence: J013024H13 - full reading 
    CCGGGGACTG CTACGGGGAC AGCACTGCCG TTTCCACCCT AGTCCAGGCA 
    GGAATTACAA GGGTTGTGGT TGGTCTTAGG CACCCCTTAA AGCACCTGAG 
    AGGAAAGGCA ATTCAATCAC TACGAAATGA AGGCATTCAA GTTGACGTTG 
    TGGGCGAGGA TCTGCACAGT AAATTATTTA AGGAAGCTCT TAAGTCATGC 
    CTCATTGTAA ATGCCCCATT ACTTTACCGA GCTGCCTTCC GTGTTCCGTT 
    CTCTGTCCTG AAGTATGCTA TGACTGCAGA TGGAAAAATT GCAGCAAGCA 
    GTGGGCATGC ATCTTGGGTA AGTGGCAAAT CATCTAGAGG CAGAGTATTT 
    GAATTGCGTG GAAGAAGCGA TGCTATAATT GTAGGTGGAA ATACAGTACG 
    CCTTGATGAT CCTCGCTTAA CTGCCAGGCA TGTCAAGGGG CATGTTCCAG 
    TGCGAATTGT AATGTCACAG TCTCTTAATC TTCCCGAGGA AGCAAATTTA 
    TGGAATGTAC ATGATGCATA CACAATAGTT GCAACACAAA GAGGTGCTCG 
    GAGAGATCTC CAAAAGAAAC TTGCTTTGAA GGGTGTTGAA GTAGTGGAGT 
    TTGACATGCT GAATCCTAGA GATGTTATGT CATATTGTTA TGATCGTGGT 
    TACCTCTCCG TATTATGGGA GTGTGGTGGG ACCCTAGCAG CTTCTGCTAT 
    ATCTGCAAGT GTTATTCATA AGGTGTATGC ATTCTTGGCT CCAAAAATAA 
    TTGGGGGAGT GAATGCTCCA ACACCAGTCG GTGAACTAGG GATGAATCAA 
    ATGACTCAAG CGATTGATTT AATTGATGTT TCTTACGAGC AGATTGATAG 
    GGACATGCTC ATGAGTGGAT TCATCCAACC TATTCCTGAT CTCTCACCTG 
    TAATACCATC TGTGGATGAA ATGCCTTCAG TTGATCCAGA AGTATCTCCA 
    TATGAAACTA ATATTATATC CTTCTACAAG ACTTGGGATA CCTTTGGTGC 
    CTTCTCTAAC TTTTCTCCCC ATCCAATTCA CATGCCCGAT GAAAATGGAG 
    ACTATTTCAC ATGGCCAACA GTGGAGCACT ATTACCAGGC ACACAAGTTT 
    ATTGGTGTTG ATGATCCTCA AGCAAGAGAG ATTGTCCAAG AAATAAAGCT 
    GGCTAAGAGT CCTGAAGAAG CTGCTAGAAT AGGACGCACT CGGCAAAGAG 
    AATTTCAAGA ATTGGTACGA CCAGATTGGG ATTCCATCAA AATTGAAGTG 
    ATGTACAGAG CTACAAAGCG CAAGTTCTCA ACTTATTCCC ATCTGACTGA 
    CATGCTGTTG TCAACGGCTG GCTCTGTTCT TGTTGAGGCG TCGCCCCACG 
    ATTTATTTTG GGGCGGTGGG CGCGAGGGCG AAGGTATGAA TTACTTGGGT 
    AGATTGCTGA TGCAATTGCG ATCAGAGATT CTAGGAACAA TTCAAACAGC 
    TGTTGAGGTA GGGGAGCCAG CTTGATTTGC TGGAGAGGTG ATCATAACAG 
    CAGACGTTTT GATCTGGGGT ATTTATATGT ATCATTATGG TGATTGCCTT 
    CAAAATTTTG TGAAACATTT GTTTCTTTAT CTCTCTAAAT TCCTGTAGAA 
    ATTTGAGATG CTTTCGTTGC TG

    Gene models with high sequence identity

    1. AT3G47390.2 [+] show detail - cytidine/deoxycytidylate deaminase family protein
      E-value: 0; Score: 708.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther Riboflavin biosynthesis protein RibD 7.90003079443043e-75
    HMMPanther PTHR11079 7.90003079443043e-75
    HMMPfam Bacterial bifunctional deaminase-reductase, C-terminal 5.79999999999997e-38
    HMMTigr Riboflavin biosynthesis protein RibD 5.39999384454126e-27
    HMMTigr Riboflavin-specific deaminase, C-terminal 8.1000073432326e-54
    HMMPfam Conserved hypothetical protein CHP02464 4.9e-41
    superfamily SSF53597 1.79999754022378e-24
    HMMTigr Conserved hypothetical protein CHP02464 1.39999277195148e-95

    See the detailed result >

    AT3G47390.2

    Model type
    Protein coding
    Short Description
    cytidine/deoxycytidylate deaminase family protein
    Curator Summary
    Encodes a protein that is believed to function as a pyrimidine reductase involved in riboflavin and FAD biosynthesis. phs1 was identified as a photosensitive mutant that shows reduced growth, chloroplast developmental abnormalities, reduced chlorophyll levels, increased oxidative stress, reduced NADPH/NADP+ ratios, reduced photosystem I electron transport, and reduced photosynthetic protein levels under high light conditions. Many of these abnormal phenotypes likely arise from the reduction in the levels of FAD in the phs1 mutant.
    Computational Description
    PHOTOSENSITIVE 1 (PHS1); CONTAINS InterPro DOMAIN/s: Conserved hypothetical protein CHP02464 (InterPro:IPR012816), CMP/dCMP deaminase, zinc-binding (InterPro:IPR002125), Cytidine deaminase-like (InterPro:IPR016193), Riboflavin-specific deaminase, C-terminal (InterPro:IPR011549), Bacterial bifunctional deaminase-reductase, C-terminal (InterPro:IPR002734), Riboflavin biosynthesis protein RibD (InterPro:IPR004794); BEST Arabidopsis thaliana protein match is: Cytidine/deoxycytidylate deaminase family protein (TAIR:AT4G20960.1).
    Link
    InterPro Scan - TAIR