K09118

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K09118

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K09118

Determination of introduced cDNA(s)

  • J023132A10 - Rice Full-length cDNAs - Original: J023132A10

    full reading [+] show sequence

    Nucleotide sequence: J023132A10 - full reading 
    GCGGGTCTCC TCTCCTCCCC CGCCGACGCG CACTCGATCC CCCCCGCCTC 
    CGCCTCCGCC TCCGCCTCCG CGTCGCCCAT CTCGAGATCC CCCGCATGGC 
    GACGCTCACC GTCCCCTCCG CCGTCCCGCC CGTCGCCGAC GACTGCGACC 
    AGCTCCGCAA GGCCTTCCAA GGGTGGGGCA CGAACGAGGC GCTCATCATC 
    TCCATCCTGG CCCACCGCGA CGCGGCGCAG CGGCGCGCCA TCCGCCGCGC 
    CTACGCCGAC ACCTACGGCG AGGAGCTCCT CCGCAGCATC ACCGACGAGA 
    TCTCCGGCGA CTTCGAGAGG GCCGTGATCC TGTGGACGCT GGACCCGGCG 
    GAGCGCGACG CGGTGCTCGC CAACGAGGTC GCGAGGAAGT GGTACCCAGG 
    GAGCGGGAGC CGCGTGCTGG TCGAGATCGC GTGCGCGCGC GGCCCCGCGC 
    AGCTGTTCGC GGTCAGGCAG GCCTACCACG AGCGCTTCAA GCGCTCGCTC 
    GAGGAGGACG TCGCGGCGCA CGCCACTGGT GACTTCCGCA AGCTCTTGGT 
    GCCACTTATA AGTGCTTACC GCTATGAGGG GCCGGAAGTC AACACAAAGT 
    TGGCACATTC AGAAGCCAAA ATTCTGCATG AGAAGATCCA GCATAAGGCA 
    TATGGTGATG ATGAGATCAT CAGAATTCTC ACTACTAGGA GCAAGGCTCA 
    GTTGATTGCG ACATTCAATC GTTACAATGA TGAATATGGT CACCCAATCA 
    ACAAGGATCT CAAGGCTGAT CCCAAGGACG AGTTCCTTTC CACGCTGCGT 
    GCAATCATCC GCTGCTTCTG TTGCCCTGAC AGGTACTTCG AGAAAGTCAT 
    CAGGTTGGCC ATCGCAGGCA TGGGAACAGA CGAGAACTCC CTCACTAGGA 
    TCATTACCAC TCGTGCCGAG GTGGATCTGA AGCTGATCAC GGAGGCGTAC 
    CAGAAGAGGA ACAGTGTCCC GCTGGAGCGT GCGGTCGCAG GGGACACCTC 
    CGGGGACTAC GAGAGGATGC TTCTTGCTCT TCTGGGTCAG GAGCAGTGAG 
    CCATGCCTAT CTTGCCCAGT CACACACTTC ATGTGATCAT GTCATATCAG 
    AGAATAAACC TGTTATGCAG GGGACACAGC CGTGGTGATT ATGATGTTGT 
    TTTTCCAGTG TACGGTACTG TTTGCTGCAG CTTGCATAAC AGTGACGATG 
    AAATAAATCA TAGTGGAATG CGTTGGCTCA TGGGACCTCA CTTATTTTGC 
    AACTTTTTGA CAGGTCTTAT TTCC

    Gene models with high sequence identity

    1. AT1G35720.1 [+] show detail - annexin 1
      E-value: 8e-101; Score: 364.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Annexin repeat, conserved site 0
    HMMPfam Annexin repeat 3.4e-21
    HMMPfam Annexin repeat 5.1e-14
    HMMPfam Annexin repeat 1.2e-16
    HMMPfam Annexin repeat 1.1e-20
    Gene3D Annexin repeat 1.4e-20
    Gene3D Annexin repeat 5.7e-14
    Gene3D Annexin repeat 1.7e-27
    Gene3D Annexin repeat 4.9e-18
    HMMPanther Annexin like protein 7.7e-193
    HMMPanther Annexin 7.7e-193
    FPrintScan Annexin 3.3e-21
    FPrintScan Annexin 3.3e-21
    FPrintScan Annexin 3.3e-21
    FPrintScan Annexin 3.3e-21
    FPrintScan Annexin, type plant 2.8e-19
    FPrintScan Annexin, type plant 2.8e-19
    FPrintScan Annexin, type plant 2.8e-19
    superfamily Annexin 7.2e-104
    HMMSmart Annexin repeat 7.8e-20
    HMMSmart Annexin repeat 2.5e-14
    HMMSmart Annexin repeat 0.021
    HMMSmart Annexin repeat 1.2e-22

    See the detailed result >

    AT1G35720.1

    Model type
    Protein coding
    Short Description
    annexin 1
    Curator Summary
    Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.
    Computational Description
    annexin 1 (ANNAT1); FUNCTIONS IN: in 7 functions; INVOLVED IN: in 8 processes; LOCATED IN: in 10 components; EXPRESSED IN: 31 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Annexin like protein (InterPro:IPR015472), Annexin repeat (InterPro:IPR018502), Annexin repeat, conserved site (InterPro:IPR018252), Annexin (InterPro:IPR001464), Annexin, type plant (InterPro:IPR009118); BEST Arabidopsis thaliana protein match is: annexin 2 (TAIR:AT5G65020.1); Has 3809 Blast hits to 2217 proteins in 205 species: Archae - 0; Bacteria - 0; Metazoa - 2893; Fungi - 160; Plants - 482; Viruses - 0; Other Eukaryotes - 274 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR