K10122

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K10122

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K10122

Determination of introduced cDNA(s)

  • J033091E04 - Rice Full-length cDNAs - Original: J033091E04

    full reading [+] show sequence

    Nucleotide sequence: J033091E04 - full reading 
    GCCGCTCCAC TTTGCTTGCC ACCACGGTCT CCCTCCCTCC CTCCCCTCTC 
    CCATCCAAAC CCCACAAAAC CGCGACGAGG AGACCCCGCC GCGACGCAGC 
    CGCAGCTCCG CCGGGGGGGG GGGGGGGGGG AGCAACGCCG CCGTGGAGTC 
    CCCTGCCACC CACGAGGGTT CCTGGTGCCT ACTTGGTCTG AATGGCTGAC 
    CTGACCGACA TGGGCTGCTG TAGTTGTTTT TGCTTTTTAA GGAAGCCTAG 
    GGTGTCTGTA AGTCGACCTC GCGACGCTGA TGGCATCCTA TCTGAAGACT 
    TGTTGAACCA TAAATCTGCG GAGGATCCTG ATGGAAGCTT CTACACCGGG 
    GATGATCCTG ATAGAAGCTT CTACGACAGG GATGATCTCG ATAGAAGCTT 
    CTACAATGGC GATGACCCTG ACAGAAGCTT CTATGATGGA GATGATCCTG 
    ATCATCTTTA TGGAAGTGAT GATGGGCAGC CAAGGAAGAG ATCTGAAGAT 
    ATTATACTGT CAAGGGCTCA GAATGGCTTT GCATGTAGAG AAAGCCTAGT 
    TAAGGAGACT AAAAAAGTAT TTCGCTCAGA GGATGAAAAT GGCAGCAAGA 
    TGGTCAACCA ATATGTCCAC CTGGGGAAGA TTGGTTCTGG AAGCTATGGC 
    AAAGTGGTCT TATACCGAAG CATGAAAGAT GGAAAGCTTT ACGCAGTGAA 
    GGTTTTGAAT AAATCTTACA TGATGAAAGT ACGTGTTGTG CGATCAGAAA 
    CTGCCATGAC AGATGTTCTT CGGGAAGTAT CCATCATGAA AATGTTGGAT 
    CATCCTAATA TAGTAAATCT CATTGAAGTG ATTGATGACC CAAATGCAGA 
    TAAATTCTAC ATGGTTCTTG AGTATGTTGA AGGAAAAATG GTCTGTGACA 
    ATGGTTTAGG AGAAGCTACC TCCAGAAGTT ACTTGCGCGA CATAATCTCT 
    GGTGTTATGT ATCTTCATTC TCATAATATT ATTCATGGTG ATATCAAACC 
    AGACAATTTG TTGGTCACAA GCACTGGCAG TGTGAAGATA GGGGATTTCA 
    GTGTTAGTCA GATTTTTGAG GATGATGATG ATCTGCTTTG GAGGTCTCCA 
    GGTACTCCTG TTTTCACTGC ACCAGAATGC TGTCAAGGTT CAGCCTACCA 
    TGGTCGGGCA GCTGATACAT GGGCAGTTGG CGTTACTCTG TATTGTATGA 
    TTACTGGTCA CTATCCATTT CTAGGAGATA CCTTACAGGA AACTTATGAT 
    AAGATTGTCA ATGATCCGGT GCAAATACCT GACAACATGA ACCCCCAACT 
    TGCTGATTTG CTGGAAAGGC TTCTCTGCAA AGATCCAGCA AATCGTATCA 
    CCCTGCAGGC TGTAGGTGAG CATCCTTGGG TTGCTGGGGA CCAGGGGCCA 
    GTTGTTGAAT ACTTCTGTAG ATGTGGGTTT GGCCGAAGAA AGAGAGATGA 
    TCTTAAGGGA GAAGTACAAT AGCAAAGGCT TGTTCACAGT TTATCATCCG 
    ATGGTTTACT GTAGATAGGC GATGAGTGTG GCTAAACAAA GAAAGTGAGA 
    TGATCGACAA ACCGTGTTAC TCTTTTTTGT GTTCTTCCTC CCTTTACCGA 
    TTGACCTGTT GGTTGCTCGC CTCACCACCT CTGCAAGTTT TGTTGCCTAA 
    CCGAGGGAGC CCCTGTTTCC ATTTTTCTGT AGATCAATTT TGGCTGCTGC 
    AACATGTAAG CTTTTTTCAT TTGGTTGTTG AATCCAGTAT AGCAACCATT 
    CAT

    Gene models with high sequence identity

    1. AT5G60550.1 [+] show detail - geminivirus rep interacting kinase 2
      E-value: 7e-127; Score: 451.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther Calcium/calmodulin-dependent protein kinase kinase 1.8e-115
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 1.8e-115
    HMMSmart Serine/threonine-protein kinase domain 3.4e-82
    HMMSmart Tyrosine-protein kinase, catalytic domain 4.3e-12
    HMMPfam Serine/threonine-protein kinase-like domain 9.8e-61
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    superfamily Protein kinase-like domain 1.8e-81
    Gene3D no description 9e-53
    ProfileScan Protein kinase, catalytic domain 44.895

    See the detailed result >

    AT5G60550.1

    Model type
    Protein coding
    Short Description
    geminivirus rep interacting kinase 2
    Curator Summary
    Encodes a geminivirus Rep interacting kinase (GRIK; GRIK1/AT3G45240, GRIK2/AT5G60550). GRIKs are SnRK1 (SNF1-related kinases) activating kinases. Both GRIKs specifically bind to the SnRK1 catalytic subunit and phosphorylate the equivalent threonine residue in its activation loop in vitro.
    Computational Description
    geminivirus rep interacting kinase 2 (GRIK2); CONTAINS InterPro DOMAIN/s: Calcium/calmodulin-dependent protein kinase kinase (InterPro:IPR020657), Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: geminivirus rep interacting kinase 1 (TAIR:AT3G45240.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR