K15902

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K15902

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K15902

Determination of introduced cDNA(s)

  • J023060B22 - Rice Full-length cDNAs - Original: J023060B22

    full reading [+] show sequence

    Nucleotide sequence: J023060B22 - full reading
    GACTGATCAG GTCTTCAGGA GAAGGAGCTT GCTTTGTACT CCTAACTGCT 
    CCTTCCAGAC ACAGCTTTGC TACTGGATTA CTGCCAATTG AGTCTTGTTA
    TTCGGTTCCT CAATTTATCA AGTTCTTCAG AGCAGGCAGT CGAAGTCCAT
    AATGTCCAAG ACTGAGAGCA GAAAGTTGTC CGATGACTAT GAAGTTGTGG
    ATGTTCTTGG CCGGGGCGGG TTCTCAATAG TAAGGAGGGG AGTGAGCAAG
    TCTGAAGAGA AGACCCAGGT TGCCATCAAG ACTCTGAGGA GGCTCGGCCC
    GGCGATGGCG GGTATGAAGC AGGGGACAAA GCCCGTGCCC GGCTCCGGCC
    TGCCGATGTG GAAACAGGTA TCGATCTCCG ACGCGCTGCT GACGAACGAG
    ATACTCGTCA TGAGGAGGAT AGTGGAGAGC GTTGCGCCAC ATCCTAATGT
    CATCAACCTG CATGATGTGT ATGAAGATGT GCATGGTGTG CACCTCGTCC
    TTGAGCTGTG CTCGGGTGGT GAGCTTTTTG ATCGGATTGT GGGGCGTGAC
    CGGTACTCGG AGTTCGATGC GGCCTGCGTC ATTCGTCAGA TTGCTAGCGG
    GCTGGAGGCT CTTCATAAGG CAAGCATTGT ACACAGGGAC CTGAAGCCGG
    AGAATTGTCT GTTCTCTGAC AAAGATGAGA AGTCCACACT GAAGATCATG
    GATTTTGGTT TGAGTTCTGT CGAAGATTTC AGTGACCCAA TCGTCGCGCT
    GTTTGGGTCA ATAGATTATG TTTCACCGGA GGCTCTATCC AGGCAGGAGG
    TTTCAGCTGC TAGTGATATG TGGTCTGTTG GGGTAATTCT GTACATTCTT
    TTATCTGGAT GCCCACCATT TCATGCTGCA ACCAATCGAG AAAAGCAGCA
    AAGGATCCTG CAAGGTGAAT TCAGTTTTCA GGACCACACA TGGAAAACAA
    TATCTTCATC CGCGAAAGAT TTGATTTCGC GCCTTCTTTC TGTTCAACCT
    TACAAAAGGC CAACAGCAAG TGATCTTCTG AGGCGTCCTT GGGTGATTGG
    AGACTGCGCC AAGCAAGATC TCATGGATGC AGAGGTCGTC TCAAAACTGC
    AAAAGTTCAA TGCTAGAAGG AAATTGCGGG CAGCTGCGAT AGCCAGCGTT
    TTGAGCTGCA AAGTGGCATT GAGGACTAAA AGGCTGAGAA ATCTTTTAGG
    AACCCATGAC CTTACCTCAG AGGAGCTGGA TAATCTGCGG CTTCATTTTG
    GACGAATATG TGCAGATGGA GAAAACGCCA CTCTGTCAGA GTTTGAGCAG
    GTGCTGAGAG CAATGAAAAT GGACTCACTG ATTCCCCTAG CTCCCCGCGT
    ATTCGATCTG TTCGACAACA ACCGTGATGG AACCGTCGAC ATGAGAGAGA
    TCCTGTGTGG GTTCTCCAGC CTCAGGAACT CACGAGGCGA TGATGCTCTT
    CGCTTGTGCT TCCAGATGTA CGACGCTGAT CGGTCAGGCT GCATCAGCAA
    GGAAGAGCTG GCATCAATGC TCCGAGCGTT GCCGGAGGAG TGCCTGCCAG
    GCGACATAAC GGAGCCGGGG AAGCTGGACG AGGTGTTCGA CCAGATGGAC
    GCCGACAGCG ACGGCAAGGT CACCTTCGAC GAGTTCAAGG CCGCCATGAA
    CAAGGACAGC GCCCTCCAGG ACGTCCTCCT CTCCTCCCTC CGCCCACAGT
    AGCAGCAGCA GCAGCAGCAG AAGCTTCTCA GATTGATCGA TTTCTTCAGG
    AAAAATACTA GTATCTGTAC TGCTAATACT TCTGCAGCGA ATGGCTGGCT
    GCCTTATCTG AAATTTTCAT CTGTGTCGGC TGCTGAAATG GTAGCAAAAC
    TGTAGCTGTT CTGTTGTCAC CTCGTCCGAA AAAATTATGA GCGGTGCGGA
    TTTCACATTT TCACTATATT CTGGGTGAAA TTGAATAGCA AGCCGGGTAA
    CATATTTACC TGATTTCC

    Gene models with high sequence identity

    1. AT5G23580.1 [+] show detail - calmodulin-like domain protein kinase 9
      E-value: 2e-67; Score: 254.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Recoverin 1e-08
    FPrintScan Recoverin 1e-08
    FPrintScan Recoverin 1e-08
    FPrintScan Recoverin 1e-08
    HMMPfam Serine/threonine-protein kinase-like domain 7.4e-60
    HMMPfam EF-hand 3.1e-07
    Gene3D no description 1.2e-57
    Gene3D EF-hand-like domain 3.5e-33
    superfamily Protein kinase-like domain 3.3e-77
    superfamily EF-hand 3.7e-40
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    ProfileScan Protein kinase, catalytic domain 46.138
    ProfileScan EF-HAND 2 8.767
    ProfileScan EF-HAND 2 13.202
    ProfileScan EF-HAND 2 13.955
    HMMPanther Calcium-dependent protein kinase 1.1e-110
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 1.1e-110
    HMMSmart Serine/threonine-protein kinase domain 7e-88
    HMMSmart Tyrosine-protein kinase, catalytic domain 2.3e-12
    HMMSmart Calcium-binding EF-hand 2.1
    HMMSmart Calcium-binding EF-hand 0.00017
    HMMSmart Calcium-binding EF-hand 1.6e-05

    See the detailed result >

    AT5G23580.1

    Model type
    Protein coding
    Short Description
    calmodulin-like domain protein kinase 9
    Curator Summary
    unique family of enzymes containing a single polypeptide chain with a kinase domain at the amino terminus and a putative calcium-binding EF hands structure at the carboxyl terminus; recombinant protein is fully active and induced by Ca2+
    Computational Description
    calmodulin-like domain protein kinase 9 (CDPK9); FUNCTIONS IN: in 6 functions; INVOLVED IN: protein amino acid phosphorylation; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 2 (TAIR:AT1G35670.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR